Long Term Preservation of Commercial Important Fungi in Glycerol at 4°C

Shankar, P.; Tiwari, K. L.; Jadhav, S. K.

doi:10.3923/ijbc.2015.79.85

Cited by 30 publications

(14 citation statements)

References 12 publications

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“…Therefore, the isolation procedures, conservation and use of microorganisms, although established in laboratory routine, are important for the research development and obtaining products of economic interest [11]. In such a way, the method of maintenance of microorganisms requires that these should be conserved under low biological activity, focusing on the preservation of sporulation and pathogenicity characteristics [12].…”

Section: Introductionmentioning

confidence: 99%

Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

Silva

Marques

Muniz

et al. 2021

JSRR

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After long periods of storage, plant pathogen isolates lose their sporulation capacity. The objective of this study was to evaluate re-isolation methodologies for recovering sporulation of Pestalotiopsis grandis-urophylla isolates after subjection to a long period of storage. Isolates of P. grandis-urophylla were kept for 14 months on Petri dishes with PDA medium at 10°C. After this period, the isolate colonies showed reduced mycelial growth and no sporulation. The isolates were inoculated on healthy Eucalyptus grandis-urophylla leaves, and after ten days they were subjected to three re‑isolation methods: scraping of the lesions (S) removing of injured plant tissue fragments, followed by disinfestation (D) and without disinfestation (WD). Then, the purified isolates were evaluated for the recovery of its sporulation ability. The different methods for re-isolation resulted in the occurrence of differences among the isolates, showing that sporulation is an isolate-dependent feature. The three methods (S, WD and D) allowed the sporulation recovery of P. grandis-urophylla, even after these isolates have been subjected to 14 months.

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Section: Introductionmentioning

confidence: 99%

Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

Silva

Marques

Muniz

et al. 2021

JSRR

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“…Water may be played a role for reducing fungal metabolism to very low levels by preventing desiccation and diminishes gas exchange. Furthermore, the current study revealed that preservation in glycerol 30% at RT (G30%.RT) could be considered as alternative method for H. maydis preservation since it exhibited a good degree of stability of the tested characteristics (Paul et al, 2015). However, Shearer et al (1974) reported that 24 strains of Septoria stored in soil up to 1 year were recovered by 100% with stability of their pathogenicity.…”

Section: Discussionmentioning

confidence: 77%

“…After that, 1.8 mL of the suspension with parts of colonized agar of each concentration were added individually in each unit (15 vials for each treatment). Then vials of each concentration of each isolate were stored at RT, 4°C and -20°C for 3, 12 and 24 months (Paul et al, 2015).…”

Section: Preservation In 10 and 30% Glycerolmentioning

confidence: 99%

Viability, Growth and Virulence of Harpophora maydis Isolates Preserved Under Different Storage Methods

El-Naggar

Yassin

2021

Egyptian Journal of Phytopathology

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Two isolates of Harpophora maydis, the causal agent of maize late wilt, were evaluated for viability, morphological characters, radial growth and virulence using twenty storage methods for 3, 12 and 24 months. Colonized discs preserved in sterile distilled water at room temperature (RT) from 25 to 32°C (SDW.RT) was the best treatment since the two isolates were recovered from all samples during the storage periods, with 90, 90 and 82.5%, respectively with no negative effect on their radial growth, morphological characters and virulence compared with the original isolates. Also, storage in 30% glycerol at room temperature (G30%. RT) exhibited a good degree of survivability (from 60 to 90%) and the examined characters were not altered. Unfortunately, fungal isolates were not able to recover after three months of storage when preserved by the methods of potato dextrose yeast agar with paraffin oil at RT, G30%. 4°C, G30%. -20°C and silica gel at -20°C. Based on the statistical analysis of the obtained results, it could be concluded that SDW.RT is simple, reliable and economic preservation method for H. maydis, moreover preservation in G30%.RT could be used along with SDW.RT or as alternative method for the tested fungus.

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“…The isolate was maintained on Sabouraud's dextrose agar (Oxoid, Basingstoke, Hamshire, UK) slopes and stored at 4 °C. Glycerol (50%) stocks were prepared for long‐term preservation at −80 °C 16 . The reference strain for antimicrobial activity assessment, S. aureus ATCC 25923, was obtained from the American Type Culture Collection (ATCC).…”

Section: Methodsmentioning

confidence: 99%

“…Clinical isolate of C. albicans CI-24 was obtained from El-Demerdash Hospital, Cairo, Egypt. The isolate was identified by sequencing of the 28S rRNA genes and the sequence assembly was conducted using Bioedit 16 The reference strain for antimicrobial activity assessment, S. aureus ATCC 25923, was obtained from the American Type Culture Collection (ATCC).…”

Section: Microorganismsmentioning

confidence: 99%

Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by Candida albicans CI‐24 via gamma irradiation and model‐based optimization

Elleboudy

Elkhatib

Yassein

et al. 2021

Biotech and App Biochem

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Para‐hydroxybenzoic acid (PHBA) has great potential in biological applications due to its putative antiviral activity against SARS‐CoV‐2 and its antimicrobial activity in the face of the radically increasing number of multidrug‐resistant pathogens. This is in addition to its antimutagenic, anti‐inflammatory, antioxidant, hypoglycemic, antiestrogenic, and antiplatelet aggregating activities. In this study, an approximate sixfold increase in the production of PHBA was achieved via biotransformation of caffeic acid by Candida albicans. The improvement was performed in two steps: first, through mutation by gamma irradiation (5 KGy dose), resulting in the recovery of a mutant (CI‐24), which produced approximately triple the amount of PHBA produced by the wild‐type isolate. Then, biotransformation by this mutant was further optimized via response surface methodology model‐based optimization. The maximum PHBA production (7.47 mg/mL) was obtained in a fermentation medium composed of 1% w/v yeast extract as a nitrogen source, with an initial pH of 6.6, incubated at 28 °C at an agitation rate of 250 rpm. To further enhance the performance and economics of the process, cells of the CI‐24 mutant were immobilized in calcium alginate beads and could retain an equivalent biotransformation capacity after three successive biotransformation cycles.

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Long Term Preservation of Commercial Important Fungi in Glycerol at 4°C

Cited by 30 publications

References 12 publications

Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

Re-Isolation Methodologies for Recovering Sporulation of Eucalyptus Pestalotiopsis grandis-urophylla Isolates after 14 Months Storage

Viability, Growth and Virulence of Harpophora maydis Isolates Preserved Under Different Storage Methods

Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by Candida albicans CI‐24 via gamma irradiation and model‐based optimization

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