Nowadays, water scarcity is a great danger to agriculture development, in semiarid and arid regions beside climate change risks. Egypt is suffering from scarce water resources for agriculture; it is of high priority to rationalize irrigation water use. During two seasons, 2019/2020 and 2020/2021 the experimental were carried out to investigate the effect of three different irrigation water treatments (I1 at 45, I2 at 60 and I3 at 75% depletion of available soil moisture) on some photosynthetic, agronomical, grain quality parameters, water productivity for eight barley genotypes. Also, classify them on the SSR molecular level. The results showed that there were high genetic variations found among the eight genotypes with significant responses to irrigation water treatments. Rationalize irrigation water from I1 to I3 had a negative effect on all studied phenotypic traits, whereas had an appositive effect on leaf diffusive resistance and cured protein content and inducement all genotypes to flower early by average increasing (27.7, 12.7 and 7.08%) respectively. Giza 138, Giza 131, and Line 4 gave high mean performance values of the measured characters besides, attaining high WP values were 1.14, 1.08, and 0.89 kg grain/m3 applied water I1, I2, and I3 irrigation treatments, respectively. While, WUE values increased with increasing water availability. Water use efficiency values were 1.92, 1.71, and 1.34 kg grain/m3 consumed water for I1, I2, and I3 irrigation treatments, respectively. Twenty-six alleles were generated using ten SSR primers with a mean value of 2.6 alleles per locus. The Polymorphism Information Content (PIC) value of each SSRs marker ranged from 0.33 (Bmag 0387) to 0.47 (Bmac 0167) with an average value of 0.34. Cluster analysis clustered the eight barley genotypes into two major clusters divided according to their response to water stress tolerance. The genetic information about eight barley genotypes for water stress tolerance was established, for use them in breeding programs in Egypt.
Greenhouse experiments were conducted in 2008, 2009 and 2010 growing seasons to determine the incidence of symptomatic and asymptomatic infection by Cephalosporium maydis the causal agent of late wilt disease of maize. The percentage of healthy appearing plants that incubating the fungus (asymptomatic) ranged from 5 to 68% whereas visually infected plants (symptomatic) was in between 0 to 100%. There was no positive correlation between the degree of resistance and asymptomatic infection. Based on the occurrence of asymptomatic infection by C. maydis, under inoculum of single isolate, mixture isolates and different levels of its density, on resistant or susceptible genotypes it is concluded that asymptomatic of late wilt disease is evident and maize plants used tolerance as a defense against the causal agent of late wilt disease.
Drought tolerance is a main trait for growing and stabilizing barley productivity in dry areas globally. The current study was conducted to evaluate the morphological and yield-related traits of the barley cultivar "Giza134" in response to drought stress. To determine the impact of drought and stress, the experiment was conducted in the growth chamber and in rainfed conditions. In the growth chamber, seedlings were irrigated, watered (normally), and subjected to 30% PEG-600 (polyethylene glycol 600) as a drought stress condition. Furthermore, barley plants were evaluated during two consecutive seasons, 2021 and 2022, at Nubaria (normal condition), in addition to two different rainfed locations on the northwest coast of Egypt, West Barrani and East Matrouh. Most morphological and yield component traits declined significantly, including plant height (cm), spike length (cm), number of grains per spike, biological yield (BY; ton/fed-1), and grain yield (GY; Ardab Fed-1). Grain yield losses were over 85% in West Barrani and East Matrouh, respectively, compared with Nubaria. To understand the mechanisms of drought tolerance at the molecular level, the gene expression of drought-responsive genes, including HvAPX1 encodes peroxidase, HvFNR encodes ferredoxin-NADP+ reductase, HvDHN1 encodes dehydrin, HvSAM encodes Sadenosyl-L-methionine methyltransferases, HvEDE encodes ER degradation enhancer, and HVABH encodes alpha/beta-hydrolases, were measured in leaf tissues of "Giza 134." The relative expression levels of HvAPX1, HvFNR, and HvDHN1 were significantly (p 0.01) upregulated, with over 8-fold for HvDHN1. while HvSAM, HvEDE and HVABH genes are downregulated in response to drought stress. These findings might provide new insights into the mechanisms of drought tolerance in barley and facilitate future breeding programs for resilient barley crops in a changing global climate.
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