Long-term preservation of adipose aspirates after conventional lipoplasty

Abstract

Our results indicated that an optimal cryopreservation approach that utilizes a combination of DMSO and trehalose as cryoprotective agents appears to provide good long-term preservation of adipose aspirates obtained from conventional lipoplasty, albeit not as ideal as fresh specimens. An in vivo study will be conducted to confirm the results from our present in vitro study.

Adipose Aspirates as a Source for Human Processed Lipoaspirate Cells after Optimal Cryopreservation

“…8 For the cryopreservation group, 1 cc of adipose aspirates after preparation was put into a 3-cc vial and mixed with 1 cc of a combined dimethyl sulfoxide (in 0.5 M) and trehalose (in 0.2 M) solution. After adding cryoprotective agents, the vial was placed in room temperature for 10 minutes and then put into a methanol bath (Kinetics, Stone Ridge, N.Y.).…”

“…and 0.2 M (7.6%) trehalose (Sigma), was used in this study according to our previous study. 8 Double concentrations of the different cryoprotective agent solutions were made first, and each chosen cryoprotective agent was then freshly diluted to the final concentration approximately 30 minutes before it was added to the adipose tissue.…”

“…These cells, according to current protocol, must be preserved and then banked for potential future applications. 9 -11 Because adipose tissue may be preserved successfully by an optimal cryopreservation method for possible future repeated autologous fat transplantation, 8 it appears to be more attractive to many patients to have their adipose aspirates preserved initially so that they may have an option later to undergo future repeated autologous fat transplantations for cosmetic or reconstructive soft-tissue augmentation. However, whether previously cryopreserved adipose tissues can still be processed later to produce processed lipoaspirate cells, possible multipotent human stem cells, after an optimal cryopreservation remains unknown.…”

“…6,7 Most recently, the long-term preservation of autologous fatty tissues for possible future transplantations has been studied in our laboratory, and adipose tissues collected from conventional liposuction can be preserved and stored successfully at low temperature (below -85°C) by means of an optimal cryopreservation technique. 8 Such a novel approach will effectively preserve adipose tissues collected from liposuction and bank these tissues for possible future clinical applications (i.e., autologous fat transplantation).…”

Clinical grade adult stem cell banking

“…79 In fact, cryopreservation of fat tissue is not a new research area and has been extensively investigated in large scale adipose tissue engineering, especially in applications of maxillofacial and craniofacial surgery. Although many studies found that freezing fat grafts by using optimal cryopreservation techniques enables their longterm preservation, [80][81][82][83][84][85][86] only few considered whether the frozen fat tissue could still be a reliable source of adult stem cells. 87,88 Further studies are required to determine whether cryopreserved fat tissue still maintains the viable and potent adult stem cells in quantities required for clinical needs.…”