Adipose Aspirates as a Source for Human Processed Lipoaspirate Cells after Optimal Cryopreservation

Pu, Lee Li-Qun; Cui, Xianwei; Fink, Betsy F.; Gao, Dayong; Vásconez, Henry C.

doi:10.1097/01.prs.0000209931.24781.9c

Cited by 41 publications

(29 citation statements)

References 23 publications

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“…Diferentes grupos de investigación han realizado trabajos que permiten obtener células madre de distintos tejidos humanos, como médula ósea y cordón umbilical (3)(4)(5)(6)(7)(8)(9). En nuestro estudio, hemos obtenido MSCs directamente del tejido graso aspirado mediante liposucción, siendo esta técnica mucho más sencilla y menos agresiva para el paciente que otros métodos (7), y que por lo tanto, puede ser fácilmente incluida como una técnica más de práctica habitual dentro de nuestra especialidad.…”

Section: Discussionunclassified

“…El tejido adiposo es uno de los tejidos más abundantes del cuerpo humano; su acceso es sencillo, el proceso para su extracción es fácil de realizar y en la mayoría de las personas, es posible extraer suficiente grasa sin perjuicio estético alguno (5,11).…”

Section: Discussionunclassified

“…Las células madre se emplean actualmente en medicina regenerativa y se espera que en un futuro más o menos próximo se puedan utilizar en el tratamiento de enfermedades como la diabetes, enfermedades cardiovasculares, lesiones medulares y enfermedades neuroló-gicas (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). Todo parece indicar que la medicina del futuro estará ligada a la medicina regenerativa y a la utilización de células madre autólogas.…”

Section: Introductionunclassified

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Obtención y criopreservación de células madre del tejido graso mediante liposucción

Ribo¹

2011

Cir. plást. iberolatinoam.

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ResumenExisten diferentes técnicas para la obtención directa de células madre embrionarias a través de embriones criopreservados en nitrógeno líquido (-196 °C), blastómeros individuales, activación de ovocitos por transferencia nuclear y células madre del líquido amniótico. También es posible obtener cé-lulas madre adultas hematopoyéticas y de la médula ósea, más conocidas y empleadas en la clínica desde hace tiempo, así como también de la sangre del cordón umbilical en recién nacidos y de la grasa corporal en adultos.La grasa autóloga corporal extraída mediante liposucción se reutiliza en ocasiones para injertar o infiltrar otras zonas corporales y faciales como material de relleno, pero en la mayoría de los casos es desechada. Por lo general, para obtener un resultado satisfactorio en el trasplante de grasa autóloga, son necesarios varios tiempos quirúrgicos por lo que sería muy útil contar con un banco de tejido adiposo personalizado. Existen diferentes estudios que demuestran que el tejido adiposo aspirado por liposucción convencional puede preservarse y almacenarse con éxito a baja temperatura (-85 °C) en banco de tejido para futuras aplicaciones.Las células madre mesenquimales (MSCs) poseen gran capacidad de proliferación y diferenciación con un alto valor terapéutico. El tejido graso es un reservorio importante de MSCs capaces de diferenciarse en tejido óseo, cartilaginoso, muscular, etc. Si reservamos una pequeña cantidad del tejido graso lipoaspirado, podemos obtener una cantidad suficiente de MSCs y conservarlas mediante un método óptimo de criopreservación.En 2007 realizamos un estudio de la grasa lipoaspirada en 36 pacientes para demostrar y validar el método de obtención de tejido graso con su posterior traslado, manipulación y criopreservación en banco de células madre. Los resultados fueron satisfactorios y en junio del 2010 se validó el método de recogida, procesamiento y almacenamiento de una mezcla enriquecida de células madre adultas de tejido graso aspirado. Dicho método permite a todas aquellas personas que no hayan tenido la oportunidad de almacenar su cordón umbilical al nacer, almacenar MSCs obtenidas de la grasa de liposucción.La criopreservación conservará las células durante años que, en el momento de ser requeridas por el paciente, se descongelarán, pudiéndose recuperar alrededor del 80% de las mismas.Palabras clave Células madre, Liposucción, Criopreservación. Código numérico 19-266 AbstractThere are different techniques to obtain embryonic stem cells directly through cryopreserved embryos in liquid nitrogen (-196ºC), individual blastomers, ovocyte activation by nuclear transfer and stem cells from amniotic fluid, among others. It is also possible to obtain hematopoietic adult stem cells and those from the bone marrow, better well-known and used in clinics for a long time; besides, from the blood of umbilical cord in newborns and body fat in adults.The autologous fat extracted from the body using liposuction, is sometimes reused to inject or infiltrate other body and facial areas as ...

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Section: Discussionunclassified

Section: Introductionunclassified

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Obtención y criopreservación de células madre del tejido graso mediante liposucción

Ribo¹

2011

Cir. plást. iberolatinoam.

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“…34 The viability of ADSC is, however, apparently not altered after cryopreservation of the aspirated material. 35 Despite the existence of very few clinical studies, the number of ADSC appears to remain the same in relation to the anatomical region. 32 Nonetheless, the human adipose tissue shows metabolic differences, according to the anatomical localization.…”

Section: Ved Stem Cellsmentioning

confidence: 99%

“…However, this number of ADSC may vary depending on the method for harvesting stem cells. In fact, data from the literature about the isolation of ADSC indicate that according to the surgical procedure [IV] and the laboratorial method employed, a different number of ADSC may be obtained from the same amount of adipose tissue, [30][31][32][33][34][35] as well as ADSC with varied characteristics and viability. 34 The viability of ADSC is, however, apparently not altered after cryopreservation of the aspirated material.…”

Section: Ved Stem Cellsmentioning

confidence: 99%

Células-tronco derivadas de tecido adiposo humano: desafios atuais e perspectivas clínicas

Yarak

Okamoto

2010

An. Bras. Dermatol.

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Adult or somatic stem cells hold great promise for tissue regeneration. Currently, one major scientific interest is focused on the basic biology and clinical application of mesenchymal stem cells. Adipose tissue-derived stem cells share similar characteristics with bone marrow mesenchymal stem cells, but have some advantages including harvesting through a less invasive surgical procedure. Moreover, adipose tissue-derived stem cells have the potential to differentiate into cells of mesodermal origin, such as adipocytes, cartilage, bone, and skeletal muscle, as well as cells of non-mesodermal lineage, such as hepatocytes, pancreatic endocrine cells, neurons, cardiomyocytes, and vascular endothelial cells. There are, however, inconsistencies in the scientific literature regarding methods for harvesting adipose tissue and for isolating, characterizing and handling adipose tissue-derived stem cells. Future clinical applications of adipose tissue-derived stem cells rely on more defined and widespread methods for obtaining cells of clinical grade quality. In this review, current methods in adipose tissue-derived stem cell research are discussed with emphasis on strategies designed for future applications in regenerative medicine and possible challenges along the way. Key words: Adipocytes; Adipose tissue; Adult stem cells; Tissue therapy Resumo: As células-tronco adultas ou somáticas detêm grande promessa para a reparação e regeneração de tecidos. Atualmente, o interesse dos cientistas é contínuo na investigação da biologia de células-tronco mesenquimais, tanto em aspectos básicos, quanto no potencial de aplicações terapêuticas. As células-tronco adultas derivadas do estroma do tecido adiposo, em comparação com as células-tronco derivadas do estroma da medula óssea, apresentam como vantagem o método fácil de obtenção da fonte tecidual. As células-tronco adultas derivadas do estroma do tecido adiposo apresentam potencial para se diferenciarem em células de tecidos mesodérmicos, como os adipócitos, as cartilagens, os ossos e o músculo esquelético e não mesodérmicos, como os hepatócitos, as células pancreáticas endócrinas, os neurônios, os hepatóci-tos e as células endoteliais vasculares. Entretanto, os dados disponíveis na literatura científica sobre as características das células-tronco adultas derivadas do estroma do tecido adiposo e os procedimentos para sua obtenção e manipulação no laboratório são inconsistentes. É necessário o desenvolvimento de metodologias e procedimentos eficazes de isolamento dessas células para obtenção de células em quantidade e qualidade suficientes para aplicação terapêutica. Nesta revisão, são discutidos os métodos correntes de coleta de tecido adiposo, isolamento e caracterização de células-tronco adultas derivadas do estroma do tecido adiposo, com ênfase na futura aplicação em medicina regenerativa e nos possíveis desafios nesse recente campo da ciência.

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Isolation, cultivation, and characterization of human mesenchymal stem cells

et al. 2017

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Mesenchymal stem cells (MSC) exhibit a high self-renewal capacity, multilineage differentiation potential and immunomodulatory properties. This set of exceptional features makes them an attractive tool for research and clinical application. However, MSC are far from being a uniform cell type, which makes standardization difficult. The exact properties of human MSC (hMSC) can vary greatly depending on multiple parameters including tissue source, isolation method and medium composition. In this review we address the most important influence factors. We highlight variations in the differentiation potential of MSC from different tissue sources. Furthermore, we compare enzymatic isolation strategies with explants cultures focusing on adipose tissue and umbilical cords as two relevant examples. Additionally, we address effects of medium composition and serum supplementation on MSC expansion and differentiation. The lack of standardized methods for hMSC isolation and cultivation mandates careful evaluation of different protocols regarding efficiency and cell quality. MSC characterization based on a set of minimal criteria defined by the International Society for Cellular Therapy is a widely accepted practice, and additional testing for MSC functionality can provide valuable supplementary information. The MSC secretome has been identified as an important signaling mechanism to affect other cells. In this context, extracellular vesicles (EVs) are attracting increasing interest. The thorough characterization of MSC-derived EVs and their interaction with target cells is a crucial step toward a more complete understanding of MSC-derived EV functionality. Here, we focus on flow cytometric approaches to characterize free as well as cell bound EVs and address potential differences in the bioactivity of EVs derived from stem cells from different sources. © 2017 International Society for Advancement of Cytometry.

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Adipose Aspirates as a Source for Human Processed Lipoaspirate Cells after Optimal Cryopreservation

Cited by 41 publications

References 23 publications

Obtención y criopreservación de células madre del tejido graso mediante liposucción

Obtención y criopreservación de células madre del tejido graso mediante liposucción

Células-tronco derivadas de tecido adiposo humano: desafios atuais e perspectivas clínicas

Isolation, cultivation, and characterization of human mesenchymal stem cells

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