Long-term intervention with Lactobacillus helveticus fermented milk reduces augmentation index in hypertensive subjects

Abstract

Background: The milk casein-derived biologically active tripeptides, isoleucyl-prolyl-proline (Ile-Pro-Pro) and valyl-prolyl-proline (Val-Pro-Pro), have documented antihypertensive effect probably related to reduced angiotensin formation. It has been suggested that these tripeptides may reduce arterial stiffness and improve endothelial function. Our aim was to evaluate whether the milk-based drink containing Ile-Pro-Pro and Val-Pro-Pro influence arterial stiffness, measured as augmentation index (AIx), and endothelial function in man. Methods: In a double-blind parallel group intervention study, 89 hypertensive subjects received daily peptide milk containing a low dose of tripeptides (5 mg/day) for 12 weeks and a high dose (50 mg/day) for the following 12 weeks, or a placebo milk drink to titrate the dose-response effect. Arterial stiffness was assessed by pulse wave analysis at the beginning and end of each intervention period. Endothelial function was tested by examining pulse wave reflection response to sublingual nitroglycerin and salbutamol inhalation. Blood pressure was measured by using office and 24-h ambulatory blood pressure measurement. Results: At the end of the second intervention period, AIx decreased significantly in the peptide group compared with the placebo group (peptide group À1.53% (95% confidence interval (CI) À2.95 to À0.12), placebo group 1.20% (95% CI 0.09-2.32), P ¼ 0 Á 013). No change in endothelial function index was observed (peptide group 0.02 (95% CI À0.06 to 0.08), placebo group 0.04 (95% CI À0.04 to 0.12), P ¼ 0.85). There were no statistically significant differences between the effects of the peptide and placebo treatment on office and 24-h ambulatory blood pressure. Conclusions: Long-term treatment with Lactobacillus helveticus-fermented milk containing bioactive peptides reduces arterial stiffness expressed as AIx in hypertensive subjects.

Antihypertensive Peptides from Food Proteins

“…In a double-blind parallel group intervention study, 89 hypertensive subjects received daily milk containing a low dose of 5 mg/day of Ile-Pro-Pro and Val-Pro-Pro for 12 weeks and a dose of 50 mg/day for the following 12 weeks. Arterial stiffness, measured by the augmentation index (AI), decreased in the peptide group by -1.53% compared to 1.20% in the placebo group at the end of the second intervention period [107]. A similar result was seen in a study by Nakamura et al [108].…”

Effect of probiotic fermented milk on blood pressure: a meta-analysis of randomised controlled trials

“…The Dietary Approaches to Stop Hypertension trial demonstrated that a dietary pattern abundant in fruits, vegetables and low-fat dairy products can considerably reduce BP (25) . Data from Study Net change 95% CI Weight (%) Hata (1996) (6) Agerholm-Larsen (2000) (7) Kawase (2000) (8) Inoue (2003) (9) Seppo (2003) (10) Mizushima (2004) (11) Tuomilehto (2004) (12) Aihara (2005) (normotensive) (13) Aihara (2005) (hypertensive) (13) Jauhiainen (2005) (14) Engberink (2008) (15) van der Zander (2008) (16) Jauhiainen (2010) (17) Usinger ( Study Net change 95% CI Weight (%) Hata (1996) (6) Agerholm-Larsen (2000) (7) Inoue (2003) (9) Seppo (2003) (10) Mizushima (2004) (11) Tuomilehto (2004) (12) Aihara (2005) (normotensive) (13) Aihara (2005) (hypertensive) (13) Jauhiainen (2005) (14) Jauhiainen (2010) (17) Usinger (2010 Fig . 3.…”

Impact of transglutaminase reaction on the immunoreactive and sensory quality of yoghurt starter

“…Some of them cause the decrease of blood pressure (Seppo et al 2003;Jauhiainen et al 2010), possess an antioxidant (Suetsuna et al 2000;Pena-Ramos et al 2004;Hernández-Ledesma et al 2007), immunomodulative properties (Meizel 2004;Phelan et al 2009a, b), and inhibit the development of cancer cells (Aimutis 2004;de LeBlanc et al 2005;Davis and Milner 2009). They cause a significant increase in the number of cells capable of producing the secretion of IgA in intestinal mucus (Matar et al 2001), and also decrease the secretion of IL-4 by lymphocytes.…”