Long-term functional reconstruction of segmental tracheal defect by pedicled tissue-engineered trachea in rabbits

Luo, Xiang; Liu, Yi; Zhang, Zhiyong; Tao, Ran; Liu, Yu; He, Aijuan; Yin, Zhen Yu; Li, Dan; Zhang, Wenjie; Liu, Wei; Cao, Yilin; Zhou, Guangdong

doi:10.1016/j.biomaterials.2013.01.060

Cited by 82 publications

(76 citation statements)

References 41 publications

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“…Epithelization provides critical functions such as the prevention of bacteria, as well as ciliary beating which helps enable discharge of secretion. In order to induce vascularization and epithelization which could aid in the long-term survival of TET transplants, several approaches exist, including pre-vascularization in vivo 21, 25 , pretreating the scaffold or cell-scaffold interface with growth factors (such as VEGF) 22, 23 , and/or using human turbinate mesenchymal stromal cell sheets as an inner surface layer in TET for epithelial regeneration 13 .…”

Section: Discussionmentioning

confidence: 99%

Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Gao

Zhang

Dong

et al. 2017

Sci Rep

103

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Long segmental repair of trachea stenosis is an intractable condition in the clinic. The reconstruction of an artificial substitute by tissue engineering is a promising approach to solve this unmet clinical need. 3D printing technology provides an infinite possibility for engineering a trachea. Here, we 3D printed a biodegradable reticular polycaprolactone (PCL) scaffold with similar morphology to the whole segment of rabbits’ native trachea. The 3D-printed scaffold was suspended in culture with chondrocytes for 2 (Group I) or 4 (Group II) weeks, respectively. This in vitro suspension produced a more successful reconstruction of a tissue-engineered trachea (TET), which enhanced the overall support function of the replaced tracheal segment. After implantation of the chondrocyte-treated scaffold into the subcutaneous tissue of nude mice, the TET presented properties of mature cartilage tissue. To further evaluate the feasibility of repairing whole segment tracheal defects, replacement surgery of rabbits’ native trachea by TET was performed. Following postoperative care, mean survival time in Group I was 14.38 ± 5.42 days, and in Group II was 22.58 ± 16.10 days, with the longest survival time being 10 weeks in Group II. In conclusion, we demonstrate the feasibility of repairing whole segment tracheal defects with 3D printed TET.

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Section: Discussionmentioning

confidence: 99%

Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Gao

Zhang

Dong

et al. 2017

Sci Rep

103

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“…In order to achieve these results, many cell types have been used alone or in combination including chondrocytes, epithelial cells, fibroblasts, adipose tissue, chondrocytes, mesenchymal amniocytes, and mesenchymal stem cells (bone-marrow mononuclear cells). 17,20,26–28,34,38,48–58 …”

Section: Discussionmentioning

confidence: 99%

Clinical Translation of Tissue Engineered Trachea Grafts

Chiang

Pepper

Best

et al. 2016

Ann Otol Rhinol Laryngol

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Objective To provide a state-of-the-art review discussing recent achievements in tissue engineered tracheal reconstruction. Data Sources and Review Methods A structured PubMed search of the current literature up to and including October 2015. Representative articles that discuss the translation of tissue engineered tracheal grafts (TETG) were reviewed. Conclusions The integration of a biologically compatible support with autologous cells has resulted in successful regeneration of respiratory epithelium, cartilage, and vascularization with graft patency, although the optimal construct composition has yet to be defined. Segmental TETG constructs are more commonly complicated by stenosis and delayed epithelialization when compared to patch tracheoplasty. Implications for Practice The recent history of human TETG recipients represents revolutionary proof of principle studies in regenerative medicine. Application of TETG remains limited to a compassionate use basis; however, defining the mechanisms of cartilage formation, epithelialization, and refinement of in vivo regeneration will advance the translation of TETG from the bench to the bedside.

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“…The rabbit trachea is known as very reactive, therefore, we chose this species to assess tracheal wall responses to metallic stents 16 . Furthermore, percutaneous stenting in this animal model is an easy, quick and effective procedure.…”

Section: Discussionmentioning

confidence: 99%

Tracheal Self-Expandable Metallic Stents: A Comparative Study of Three Different Stents in a Rabbit Model

Serrano

Lostalé

Rodríguez-Panadero

et al. 2016

Archivos de Bronconeumología (English Edition)

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Long-term functional reconstruction of segmental tracheal defect by pedicled tissue-engineered trachea in rabbits

Cited by 82 publications

References 41 publications

Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Tissue-engineered trachea from a 3D-printed scaffold enhances whole-segment tracheal repair

Clinical Translation of Tissue Engineered Trachea Grafts

Tracheal Self-Expandable Metallic Stents: A Comparative Study of Three Different Stents in a Rabbit Model

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