Long-term exposure to nanoplastics alters molecular and functional traits related to the carcinogenic process

Barguilla, Irene; Domenech, Josefa; Ballesteros, Sandra; Rubio, Laura; Marcos, Ricard; Hernández, Alba

doi:10.1016/j.jhazmat.2022.129470

Cited by 16 publications

(12 citation statements)

References 44 publications

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“…Nevertheless, PSNPLs generated DNA damage by both oxidative and non-oxidative mechanisms after the 12-week chronic exposure. The same authors also demonstrated that a longer period of exposure to the same NPLs (24 weeks) was able to alter early tumoral phenotype biomarkers [33]. Changes in stress-response-related genes were observed with a significant upregulation of Gstp-1 and a downregulation of Keap, Nrf2, Pgp, Sod1, and Sod2.…”

Section: In Vitro Studies On Carcinogenicity-related Effectsmentioning

confidence: 83%

“…No studies performed with validated guidelines (i.e., the Bhas-42 cell transformation assay) were found in our search. Furthermore, only two studies [33,34], evaluated the transforming capacity of MNPLs using in vitro long-term approaches and a wide battery of different hallmarks of cancer. The selection of bioassays for the evaluation of carcinogenesis is of paramount importance.…”

Section: Discussionmentioning

confidence: 99%

“…Three studies focused on the potential carcinogenic risk of MNPLs in vitro, by using cell transformation approaches, have been recently published. Two of these studies used mouse embryonic fibroblasts deficient in repairing oxidized DNA base lesions (MEF Ogg1 -/-) in a chronic exposure scenario with exposures lasting for 12 and 24 weeks to relatively low doses of PSNPLs (0.05 µm; 25 µg/mL) [33,34]. The authors reported, after 12 weeks of chronic exposure to the co-treatment with arsenic (2 µM As III ), a well-known carcinogen in humans, a synergistic effect increasing a set of cancer hallmarks, such as the number of colonies formed in the anchorage-independent growth assay, and the acquisition of invasion and migration abilities, although PSNPLs alone did not induce these effects [34].…”

Section: In Vitro Studies On Carcinogenicity-related Effectsmentioning

confidence: 99%

See 2 more Smart Citations

Insights into the potential carcinogenicity of micro- and nano-plastics

Domenech

Annangi

Marcos

et al. 2023

Mutation Research/Reviews in Mutation Research

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Section: In Vitro Studies On Carcinogenicity-related Effectsmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: In Vitro Studies On Carcinogenicity-related Effectsmentioning

confidence: 99%

See 1 more Smart Citation

Insights into the potential carcinogenicity of micro- and nano-plastics

Domenech

Annangi

Marcos

et al. 2023

Mutation Research/Reviews in Mutation Research

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“…The number of tumorspheres formed can be used to estimate the proportion of stem-like cells present in a population of transformed cells. Tumorspheres derived in vitro from tumoral cells have been described to be highly invasive and to initiate tumorigenesis when engrafted in vivo [114]. Thus, the acquisition of this sphere-forming capability is considered a marker of aggressiveness and CSC enrichment in the in vitro cell-transformed population.…”

Section: Stem-like Featuresmentioning

confidence: 99%

In Vitro Approaches to Determine the Potential Carcinogenic Risk of Environmental Pollutants

Barguilla

Maguer-Satta

Guyot

et al. 2023

IJMS

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One important environmental/health challenge is to determine, in a feasible way, the potential carcinogenic risk associated with environmental agents/exposures. Since a significant proportion of tumors have an environmental origin, detecting the potential carcinogenic risk of environmental agents is mandatory, as regulated by national and international agencies. The challenge mainly implies finding a way of how to overcome the inefficiencies of long-term trials with rodents when thousands of agents/exposures need to be tested. To such an end, the use of in vitro cell transformation assays (CTAs) was proposed, but the existing prevalidated CTAs do not cover the complexity associated with carcinogenesis processes and present serious limitations. To overcome such limitations, we propose to use a battery of assays covering most of the hallmarks of the carcinogenesis process. For the first time, we grouped such assays as early, intermediate, or advanced biomarkers which allow for the identification of the cells in the initiation, promotion or aggressive stages of tumorigenesis. Our proposal, as a novelty, points out that using a battery containing assays from all three groups can identify if a certain agent/exposure can pose a carcinogenic risk; furthermore, it can gather mechanistic insights into the mode of the action of a specific carcinogen. This structured battery could be very useful for any type of in vitro study, containing human cell lines aiming to detect the potential carcinogenic risks of environmental agents/exposures. In fact, here, we include examples in which these approaches were successfully applied. Finally, we provide a series of advantages that, we believe, contribute to the suitability of our proposed approach for the evaluation of exposure-induced carcinogenic effects and for the development of an alternative strategy for conducting an exposure risk assessment.

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“…Previous studies reported that small-sized plastic debris, especially NPs, could interrupt and cross biological barriers to damage cell membrane. − Moreover, they tend to adsorb more chemical pollutants due to a high specific area, and these contaminations are indirectly transferred to the organisms. − Jin et al reported that microbiota dysbiosis and inflammation in the adult zebrafish gut exposed to 0.5 μm commercial polystyrene (PS) was more severe than that exposed to 50 μm after 14 days . In mammals, carboxylate-modified PS NPs (200 nm) were found in the interstitial compartment surrounding the seminiferous tubules of the male mice by oral administration, and genotoxicity tests indicated increased DNA damage in spleen tissue .…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Polypropylene Nanoplastics Via Thermal Oxidation Reaction for Human Cells Responsiveness Studies

Hiranphinyophat,

Hiraoka,

Kobayashi

et al. 2023

Langmuir

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With the current worldwide increasing use of plastics year by year, nanoplastics (NPs) have become a global threat to environmental and public health concerns. Among plastics, polypropylene (PP) is widely used in industrial and medical applications. Owing to the lack of validated detection methods and standard materials for PP NPs, understanding the impact of PP NPs on the environmental and biological systems is still limited. Here, isotactic polypropylene (iPP) was fabricated into oxidized polypropylene micro/nanoplastics (OPPs) via a thermal oxidation using hydrogen peroxide (H 2 O 2 ) under various heating temperatures. The resulting OPPs were investigated in terms of the size distribution, surface chemistry, morphology, and thermal property as well as their concentration-dependent cytotoxicity to a human intestinal epithelial cell line (Caco-2), which could be a route to uptake NPs into the body through the food chain. The average diameters of the OPPs decrease with increasing reaction temperature. The OPPs obtained at 175 °C (OPP175) were spherical in shape and had a rough surface, with size distributions of approximately 0.14 ± 0.02 μm. A significant increase in the carbonyl content of the oxidized product was confirmed by Fourier transform infrared and X-ray photoelectron spectroscopy analyses. Caco-2 cells were exposed to OPP175 in a dose-dependent manner, and a significant loss of cell viability occurred at the concentration of 100 μg/mL. Thus, this study provides a fundamental approach for the fabrication of a model of NPs for the urgently demanded in vitro and in vivo studies to assess the potential impact of NPs on biological systems.

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Long-term exposure to nanoplastics alters molecular and functional traits related to the carcinogenic process

Cited by 16 publications

References 44 publications

Insights into the potential carcinogenicity of micro- and nano-plastics

Insights into the potential carcinogenicity of micro- and nano-plastics

In Vitro Approaches to Determine the Potential Carcinogenic Risk of Environmental Pollutants

Fabrication of Polypropylene Nanoplastics Via Thermal Oxidation Reaction for Human Cells Responsiveness Studies

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