Long-term exposure to cypermethrin and piperonyl butoxide cause liver and kidney inflammation and induce genotoxicity in New Zealand white male rabbits

Vardavas, Alexander; Stivaktakis, Polychronis; Tzatzarakis, Manolis; Fragkiadaki, Persefoni; Vasilaki, Fotini; Tzardi, Maria; Datseri, Galateia; Tsiaoussis, John; Alegakis, Athanasios; Tsitsimpikou, Christina; Rakitskii, Valerii N.; Carvalho, Félix; Tsatsakis, Aristidis

doi:10.1016/j.fct.2016.06.016

Cited by 50 publications

(28 citation statements)

References 34 publications

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“…Molecular markers of cell death, bax–bcl2 ratio, cyt-c, and caspases-3, showed an increase in apoptosis compared to control upon the treatment with cypermethrin, and the exposed cells showed significant DNA damage (% tail and OTM), a characteristic of apoptosis. The induction of genotoxicity and oxidative stress as observed in our study is in accordance with the observations of Vardavas et al 17 , 18 in liver cells of male rabbit for cypermethrin and piperonyl butoxide.…”

Section: Discussionsupporting

confidence: 93%

Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study

et al. 2018

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Most of the agricultural workers are potentially exposed to pesticides through different routes. Inhalation exposures may result in numerous diseases that can adversely affect an individual’s health and capacity to perform at work. The aim of this study was to determine the cytotoxic potential of cypermethrin pesticide on cultured human hepatocarcinoma (HepG2) cells. The HepG2 cells were exposed to cypermethrin (0, 5, 15, 40 ng/mL) for 24 and 48 hours. We observed that cypermethrin caused cell death of HepG2 cells using 3-(4, 5-dimethylthiozolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase tests. Furthermore, cypermethrin reduced HepG2 cells viability in a time and dose dependent basis, that was probably mediated through the induction of reactive oxygen species (ROS) and apoptosis. An increase in ROS generation with a concomitant increase in expression of the proapoptotic protein Bcl-2 and cytochrome c and decrease in the antiapoptosis protein Bax suggested that a mitochondria-mediated pathway was involved in cypermethrin-induced apoptosis. These findings provide insights into the underlying mechanisms involved in cytotoxicity of cypermethrin in HepG2 cells.

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Section: Discussionsupporting

confidence: 93%

Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study

et al. 2018

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“…However, to the best of our knowledge, there is no study available to date investigating the effects of stanozolol on telomerase activity in the presence/absence of exercise, apart from our previous study which focused on circlulating telomerase activity in peripheral blood mononuclear cells (PBMCs) ( 35 ). Our results indicated the elevation of telomerase activity and TERT expression in the liver tissue, which could be associated either with an increased proliferation risk due to stanozolol treatment ( 10 ), rather unlikely for such a short exposure period, or may represent a counteracting mechanism ( 54 ). Exercise reverses the stanozolol-induced increase in telomerase activity.…”

Section: Discussionmentioning

confidence: 78%

Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging

et al. 2018

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Anabolic agents are doping substances which are commonly used in sports. Stanozolol, a 17α-alkylated derivative of testosterone, has a widespread use among athletes and bodybuilders. Several medical and behavioral adverse effects are associated with anabolic androgenic steroids (AAS) abuse, while the liver remains the most well recognized target organ. In the present study, the hepatic effects of stanozolol administration in rats at high doses resembling those used for doping purposes were investigated, in the presence or absence of exercise. Stanozolol and its metabolites, 16-β-hydroxystanozolol and 3′-hydroxystanozolol, were detected in rat livers using liquid chromatography-mass spectrometry (LC-MS). Telomerase activity, which is involved in cellular aging and tumorigenesis, was detected by examining telomerase reverse transcriptase (TERT) and phosphatase and tensin homolog (PTEN) expression levels in the livers of stanozolol-treated rats. Stanozolol induced telomerase activity at the molecular level in the liver tissue of rats and exercise reversed this induction, reflecting possible premature liver tissue aging. PTEN gene expression in the rat livers was practically unaffected either by exercise or by stanozolol administration.

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“…Exposure of male rabbits to two doses of cypermethrin for two and four months led to increased genotoxicity in the lymphocytes and cytotoxicity in the liver and kidney, as demonstrated by the cytokinesis-block micronucleus assay and histological examination (178).…”

Section: Pyrethroid Effects On Non-target Vertebratesmentioning

confidence: 98%

Non-target toxicity of novel insecticides

Mužinić

Želježić

2018

Archives of Industrial Hygiene and Toxicology

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Humans have used insecticides since ancient times. The spectrum and potency of available insecticidal substances has greatly expanded since the industrial revolution, resulting in widespread use and unforeseen levels of synthetic chemicals in the environment. Concerns about the toxic effects of these new chemicals on non-target species became public soon after their appearance, which eventually led to the restrictions of use. At the same time, new, more environmentally-friendly insecticides have been developed, based on naturally occurring chemicals, such as pyrethroids (derivatives of pyrethrin), neonicotinoids (derivatives of nicotine), and insecticides based on the neem tree vegetable oil (Azadirachta indica), predominantly azadirachtin. Although these new substances are more selective toward pest insects, they can still target other organisms. Neonicotinoids, for example, have been implicated in the decline of the bee population worldwide. This review summarises recent literature published on non-target toxicity of neonicotinoids, pyrethroids, and neem-based insecticidal substances, with a special emphasis on neonicotinoid toxicity in honeybees. We also touch upon the effects of pesticide combinations and documented human exposure to these substances.

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Long-term exposure to cypermethrin and piperonyl butoxide cause liver and kidney inflammation and induce genotoxicity in New Zealand white male rabbits

Cited by 50 publications

References 34 publications

Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study

Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study

Stanozolol administration combined with exercise leads to decreased telomerase activity possibly associated with liver aging

Non-target toxicity of novel insecticides

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