Long-term expansion of human neural progenitor cells by epigenetic stimulation in vitro

Zhang, Haiyan; Zhao, Yongmei; Zhao, Chunli; Yu, Shuang; Duan, Deyi; Xu, Qian

doi:10.1016/j.neures.2004.10.010

Cited by 22 publications

(24 citation statements)

References 31 publications

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“…Thus, the neurospheres in the floating culture fail to survive after the initial passage [7][8][9][10] . Trying to solve this problem, Yang et al [12] and Zhang et al [13] switched to adherent culture, which led to successful expansion of neural progenitor cells isolated from the embryonic human brain. Most of this kind of cells attach onto the bottom of the plates precoated with ornithin and laminin or poly-L -lysine and keep proliferating for at least 8 passages in 3 months.…”

Section: Discussionmentioning

confidence: 99%

“…Nestin, a marker for neurectodermal stem cells is generally accepted as a useful marker for identification of neural progenitor cells, which is found to be localized in proliferating CNS cells, and loss of nestin expression coincides with differentiation. Thus, the percentage of nestin-positive cells can be regarded as an indicator of uncommitted cell rate [13,14] . Therefore, it can be said that adherent neurosphere culture purified stem cells to some degree by screening those which had already differentiated.…”

Section: Discussionmentioning

confidence: 99%

“…Third, LIF was added to our culture system which has been reported to markedly increase the rate of cell proliferation and maintain the multipotential of neural progenitor cells [13,14] . Most importantly, it can yield more neurons than culture without LIF, which is consistent with the previously reported results [15] .…”

Section: Discussionmentioning

confidence: 99%

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Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

Lai

Tang

et al. 2008

Ophthalmic Res

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This study developed a modified monolayer culture technique for expansion of retinal progenitor cells in vitro. Using this method, spheres collected by low centrifugation from free floating cultures were re-seeded onto poly-D-lysine pre-coated flasks, and cultured in the retinal progenitor cells medium (including fibrous growth factor, epidermal growth factor and leukemia inhibitor factor) plus 5% FBS for the first 24 h, then FBS was removed. These cultured cells were proliferative, and in addition to expressing the neuroectodermal marker nestin they were multipotential. By immunocytochemistry and FACS analysis, we demonstrated that the percentage of nestin-expressing cells in passage 2 and passage 8 was 92.5 and 85.5%, respectively, which was greater than the percentage of their counterparts, 81.9 and 76.2%, in simple adhesive cultures (p < 0.05). Moreover, these cells preferentially differentiated into retinal neurons rather than glial cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

Lai

Tang

et al. 2008

Ophthalmic Res

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“…Conditional retroviral supernatants were produced by the stable retrovirus-producing cell lines PG13/eGFP as described previously [14,15]. For transfection, *1 · 10 6 PDLSCs or DPSCs grown in 75-cm 2 flasks were incubated for *20 h with a mixture of a viral supernatant and the growth medium at equal volumes and in the presence of 5 mg/mL protamine from salmon (Sigma-Aldrich).…”

Section: Transfection Of Egfp Gene (Green Fluorescent Protein Labeling)mentioning

confidence: 99%

Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine

Wei

Tao

Ding

et al. 2013

Stem Cells and Development

130

102

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“…Cells were harvested with trypsin/EDTA 24 h post-transduction and subcultured at a ratio of 1:3 into selective medium, which contained 0.5 μg/ml puromycin. hLMDF clones expressing high levels of EGFP (EGFP-hLMDFs) were isolated by cloning using trypsin/EDTA and amplified using conventional culture methods (Yamamoto et al, 2003;Zhang et al, 2005).…”

Section: Generation Of Egfp-labeled Single Cell Clone-derived Hlmdfsmentioning

confidence: 99%

Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells

et al. 2012

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Recent evidence has suggested that human skin fibroblasts may represent a novel source of therapeutic stem cells. In this study, we report a 3-stage method to induce the differentiation of skin fibroblasts into insulin-producing cells (IPCs). In stage 1, we establish the isolation, expansion and characterization of mesenchymal stem cells from human labia minora dermis-derived fibroblasts (hLMDFs) (stage 1: MSC expansion). hLMDFs express the typical mesenchymal stem cell marker proteins and can differentiate into adipocytes, osteoblasts, chondrocytes or muscle cells. In stage 2, DMEM/F12 serum-free medium with ITS mix (insulin, transferrin, and selenite) is used to induce differentiation of hLMDFs into endoderm-like cells, as determined by the expression of the endoderm markers Sox17, Foxa2, and PDX1 (stage 2: mesenchymal-endoderm transition). In stage 3, cells in the mesenchymal-endoderm transition stage are treated with nicotinamide in order to further differentiate into self-assembled, 3-dimensional islet cell-like clusters that express multiple genes related to pancreatic β-cell development and function (stage 3: IPC). We also found that the transplantation of IPCs can normalize blood glucose levels and rescue glucose homeostasis in streptozotocin-induced diabetic mice. These results indicate that hLMDFs have the capacity to differentiate into functionally competent IPCs and represent a potential cell-based treatment for diabetes mellitus.

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Long-term expansion of human neural progenitor cells by epigenetic stimulation in vitro

Cited by 22 publications

References 31 publications

Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

Long-Term in vitro Expansion of Retinal Progenitor Cells by Culturing Intact Neurospheres in Monolayer

Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine

Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells

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