Functional Tooth Restoration by Allogeneic Mesenchymal Stem Cell-Based Bio-Root Regeneration in Swine

Three-Dimensional Printed Multiphase Scaffolds for Regeneration of Periodontium Complex

“…[4][5][6][7] The field of tooth regeneration has grown robustly. [8][9][10][11][12][13][14][15][16][17][18][19][20] The ambitious goal to regenerate an entire tooth, including the enamel, has encountered several barriers, such as the unavailability of patient compatible, postnatal stem/progenitor cells, and lack of strategy to derive functional ameloblasts. 12 A less ambitious and pragmatic goal to regenerate mineralized tooth roots has gained substantial momentum.…”

“…12 A less ambitious and pragmatic goal to regenerate mineralized tooth roots has gained substantial momentum. 9,17,19 Dentin-like and cementum/PDL-like tissues were formed in vivo from dental pulp stem/progenitor cells (DPSCs) and PDL stem/ progenitor cells (PDLSCs), respectively. 11,21 A tooth rootlike structure with PDL-like tissue was formed in hydroxyapatite (HA)/tricalcium phosphate cylinder that was loaded with stem cells from apical papilla and PDLSCs.…”

“…9 Transplantation of allogeneic dental mesenchymal stem/progenitor cells in HA scaffold in extraction socket supported the function of a prosthetic crown. 19 Despite the progress in tooth root regeneration, an existing barrier is to regenerate not only multiple tissues of an anatomically correct tooth root but also the supporting PDL and alveolar bone in one integrated strategy and by using the patient's endogenous cells. 22 Biophysical properties of scaffolds, such as surface tomography, internal microstructure, scale and interconnectivity of pores/channels, and material elasticity, play important roles in cell adhesion, migration, proliferation, and differentiation.…”

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

“…In so far gradient perfusion culture appears to be an appropriate technique for exploring long term toxic effects under realistic in vitro conditions (Sengün et al 2011;Korsuwannawong et al 2012;Kim et al 2013a, b;da Silva et al 2014). A further innovative approach is tooth regeneration that was investigated by allogeneic stem cells (Wei et al 2013).…”

Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

“…The odontoblast-like cells elongated their processes into the dentin matrix, which interacted with pulp-like interstitial tissuecontaining blood vessels. Subsequent investigations transplanted DPSCs seeded in various types of scaffolds to determine the optimal scaffold for dentin/pulp tissue engineering [33][34][35]. The capacity of DPSC to induce periodontal regeneration has been investigated following DPSC transplantation into periodontal defects that were surgically developed on the mesial surfaces of dog first molars [36].…”