Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Tomokiyo, Atsushi; Hynes, Kim; Gronthos, Stan; Wada, Naohisa; Bartold, P. Mark

doi:10.1007/s40496-015-0073-8

Cited by 6 publications

(5 citation statements)

References 75 publications

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“…The neural crest is a group of cells located in the neural folds at the boundary between the neural and epidermal ectoderms. Neural crest cells (NCCs) are a transient population of multipotent progenitors that migrate throughout the body to generate a diverse array of tissues, such as the peripheral nervous system, endocrine cells in the adrenal and thyroid glands, skin melanocytes, and craniofacial cartilage and bone (7,(28)(29)(30)57). In addition, previous studies have shown that NCCs migrate to tooth-forming sites and give rise to dental mesenchymal cells, which produce dentin, dental pulp, and a part of the periodontium (7,18).…”

Section: Methodsmentioning

confidence: 99%

“…Arakaki et al reported that mouse iPS cells could differentiate into ameloblasts via interaction with the dental epithelium (3). To date, several groups have reported protocols for the differentiation of human ES or iPS cells into NCCs (4,30,31,57). Soon after, another group differentiated mouse iPS cells into NCCs and used them to generate odontoblasts (43,44).…”

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confidence: 99%

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Induction of neural crest cells from human dental pulp-derived induced pluripotent stem cells

et al. 2017

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We previously generated induced pluripotent stem (iPS) cells from human dental pulp cells of deciduous teeth. Neural crest cells (NCCs) play a vital role in the development of the oral and maxillofacial region. Therefore, NCCs represent a cell source for bone, cartilage, and tooth-related tissue engineering. In this study, we examined whether iPS cells are capable of differentiating into NCCs through modification of the human embryonic stem cell protocol. First, iPS cells were dissociated into single cells and then reaggregated in low-cell-adhesion plates with neural induction medium for 8 days in suspension culture to form neurospheres. The neurospheres were transferred to fibronectin-coated dishes and formed rosette structures. The migrated cells from the rosettes abundantly expressed NCC markers, as evidenced by real-time polymerase chain reaction, immunofluorescence, and flow cytometric analysis. Furthermore, the migrated cells exhibited the ability to differentiate into neural crest lineage cells in vitro. They also exhibited tissue-forming potential in vivo, differentiating into bone and cartilage. Collectively, the migrated cells had similar characteristics to those of NCCs. These results suggest that human dental pulp cell-derived iPS cells are capable of differentiating into NCCs. Therefore, iPS cell-derived NCCs represent cell sources for bone and cartilage tissue engineering.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Induction of neural crest cells from human dental pulp-derived induced pluripotent stem cells

et al. 2017

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“…5,6,17,18 Another source of stem cells, the oral mucosa with a very high regenerative ability following injury, and can be obtained even from patients without teeth. 23 Studies have shown that dental pulp, apical papilla, periodontal ligaments, and oral mucosa-derived stem/ progenitor cells have the characteristics of NCSCs, with each type of tissue-derived cell considered to possess identical stem/progenitor cell properties; [17][18][19][20][21][22][23][24]26 however, these differences have not been characterized. Elucidating these cell-specific characteristics may help not only in demonstrating the importance of each cell type with respect to tissue regeneration via differentiation into suitable target lineages, but also in identifying tissue-specific markers and their developmental role in the currently unknown human tooth developmental process.…”

Section: Introductionmentioning

confidence: 99%

Differences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest‐derived multilineage cells

et al. 2022

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BackgroundAlthough multilineage cells derived from oral tissue, especially the dental pulp, apical papilla, periodontal ligament, and oral mucosa, have neural crest-derived stem cell (NCSC)-like properties, the differences in the characteristics of these progenitor cell compartments remain unknown. The primary aim of the current study was to elucidate these differences. MethodsMultilineage sphere-forming apical papilla-derived cells (APDCs), periodontal ligament-derived cells (PDLDCs), and oral mucosa stroma-derived cells (OMSDCs) from the same individuals were isolated from impacted developing teeth. All sphere-forming cells were characterized by biological analyses of stem cells. Additionally, composites of these cells and multiporous hydroxyapatite scaffolds were transplanted into immunocompromised mice. ResultsAll sphere-forming cells expressed neural crest-related markers. Although APDCs and PDLDCs showed greater mineralized-cell differentiation, they exhibited poorer differentiation into adipocytes in vitro than OMSDCs. In immunocompromised mice, APDCs were better able to form hard tissues than PDLDCs and OMSDCs. Moreover, the expression of certain tissue-speci c markers, such as CD24 and CD56 (NCAM1), differed among the tissue-derived cells. Surprisingly, the expression of only CD24 and CD56 could be discriminated among human tissues. ConclusionsOur results suggest that although cells having NCSC-like properties present the same phenotype, they differ in the expression of certain markers and differentiation abilities. The present study is the rst to demonstrate the differences in differentiation ability and molecular markers among multilineage human APDCs, PDLDCs, and OMSDCs obtained from the same patients and, concomitantly, the same sites and identify these tissuespeci c markers in the human tooth developmental process.

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“…These cells maintain their multipotency as they can be coaxed to differentiate into neuronal and glial cells 10–17 , smooth muscle cells 10,12,14 , melanocytes 14,18 , bone cells 18–23 , adipocytes 10,18–20 , and chondrocytes 10,14,18 . As a result, several groups have proposed their use in applications including treatment for spinal cord injury 24 , deafness 25 , ocular repair 26–28 or periodontal regeneration 29 . However, clinical application is hampered by the need for genetic modification in reprogramming or the limited accessibility of adult tissues where they reside.…”

Section: Introductionmentioning

confidence: 99%

Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo

Boroujeni

Koontz

Tseropoulos

et al. 2019

Sci Rep

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Neural crest (NC) cells are multipotent stem cells that arise from the embryonic ectoderm, delaminate from the neural tube in early vertebrate development and migrate throughout the developing embryo, where they differentiate into various cell lineages. Here we show that multipotent and functional NC cells can be derived by induction with a growth factor cocktail containing FGF2 and IGF1 from cultures of human inter-follicular keratinocytes (KC) isolated from elderly donors. Adult NC cells exhibited longer doubling times as compared to neonatal NC cells, but showed limited signs of cellular senescence despite the advanced age of the donors and exhibited significantly younger epigenetic age as compared to KC. They also maintained their multipotency, as evidenced by their ability to differentiate into all NC-specific lineages including neurons, Schwann cells, melanocytes, and smooth muscle cells (SMC). Notably, upon implantation into chick embryos, adult NC cells behaved similar to their embryonic counterparts, migrated along stereotypical pathways and contributed to multiple NC derivatives in ovo . These results suggest that KC-derived NC cells may provide an easily accessible, autologous source of stem cells that can be used for treatment of neurodegenerative diseases or as a model system for studying disease pathophysiology and drug development.

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Is There a Role for Neural Crest Stem Cells in Periodontal Regeneration?

Cited by 6 publications

References 75 publications

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

<b>Induction of neural crest cells from human dental pulp-derived induced pluripotent stem </b><b>cells </b>

Differences in the stemness characteristics and molecular markers of distinct human oral tissue neural crest‐derived multilineage cells

Neural crest stem cells from human epidermis of aged donors maintain their multipotency in vitro and in vivo

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