Long-term delivery of protein and peptide therapeutics for cancer therapies

Sikder, Sadia; Gote, Vrinda; Alshamrani, Meshal; Sicotte, Jeff; Pal, Dhananjay

doi:10.1080/17425247.2019.1662785

Cited by 17 publications

(12 citation statements)

References 138 publications

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“…Although small, lipophilic molecules have a higher propensity toward skin penetration, many therapeutics identified in recent times are biomolecules including DNA, RNA, proteins, and polysaccharides. − In this report, we have explored the delivery of one such biomacromolecule, chondroitin sulfate (CS). CS being a negatively charged polymer with a molecular weight ranging from 15 to 50 kDa cannot diffuse passively through the skin layers because of its negative charge, hydrophilicity, and size and therefore needs active administration.…”

Section: Discussionmentioning

confidence: 99%

Topical Application of Peptide–Chondroitin Sulfate Nanoparticles Allows Efficient Photoprotection in Skin

Mishra

Pal

et al. 2021

ACS Appl. Mater. Interfaces

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In this article, we describe a method of delivery of chondroitin sulfate to skin as nanoparticles and demonstrate its anti-inflammatory and antioxidant role using UV irradiation as a model condition. These nanoparticles, formed through electrostatic interactions of chondroitin sulfate with a skin-penetrating peptide, were found to be homogenous with positive surface charges and stable at physiological and acidic pH under certain conditions. They were able to enter into the human keratinocyte cell line (HaCaT), artificial skin membrane (mimicking the human skin), and mouse skin tissue unlike free chondroitin sulfate. The preapplication of nanoparticles also exhibited reduced levels of oxidative stress, cyclobutane pyrimidine dimer formation, TNF-α, and so on in UV-B-irradiated HaCaT cells. In an acute UV-B irradiation mouse model, their topical application resulted in reduced epidermal thickness and sunburn cells, unlike in the case of free chondroitin sulfate. Thus, a completely noninvasive method was used to deliver a bio-macromolecule into the skin without using injections or abrasive procedures.

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Section: Discussionmentioning

confidence: 99%

Topical Application of Peptide–Chondroitin Sulfate Nanoparticles Allows Efficient Photoprotection in Skin

Mishra

Pal

et al. 2021

ACS Appl. Mater. Interfaces

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“…Nowadays, protein-based biologically active compounds and drugs, such as hormones, antioxidants, enzymes, enzyme inhibitors, vaccines, antitumor agents, etc., have gained wide application in medicine, food industry, and biotechnology. On the other hand, their use is associated with some limitations, the main of which are related to high sensitivity of proteins and medicines to denaturation, aggregation, or hydrolysis in the gastrointestinal tract (GIT), undesirable interactions of components of a medicine with each other or with other medicines, poor absorption of proteins in the GIT, hydrophobicity, instability and degradation on storage, toxicity, and immunogenicity of foreign protein components [1][2][3][4][5]. One of the approaches to overcome these limitations is based on encapsulation of proteins using appropriate carriers [1,[6][7][8][9][10][11].…”

Section: Encapsulation Of Proteinsmentioning

confidence: 99%

“…Variable [5,181] High [5] Relatively low Inorganic matrices 22-41% [18] 1.7% [19] Up to 20% [182] 23-100% [21] 30-280 to 2000 nm [21,22,183,184] 2-3 nm to 2-3 μm [21,180,183] Yes [180] High Insufficient data (up to 15 days) [21] Variable [19,21,22,180,[183][184][185][186]] Under extensive study [21,183,186] Low Polymersomes 10-60% [187] 5 nm-5 μm [176] -Yes [188] Up to 6 months or more [167] Depends on the structure of amphiphilic block copolymer [167] Depends on the structure of amphiphilic block copolymer [188] Variable…”

Section: Lowmentioning

confidence: 99%

“…Other disadvantages of using vesicular systems, including liposomes, as well as solid lipid particles, are their high cost, laborious production, susceptibility to degradation during storage, and the need to control aggregation of colloidal particles. Until now, problems associated with limited drug load volume of micellar systems and stability of formulations encapsulated therein have not been resolved [ 2 , 5 , 10 , 26 , 33 ].…”

Section: Encapsulation Of Proteinsmentioning

confidence: 99%

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Methods of Encapsulation of Biomacromolecules and Living Cells. Prospects of Using Metal–Organic Frameworks

et al. 2021

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The review discusses different methods of encapsulation and biomineralization of macromolecules and living cells. Main advantages and disadvantages of most commonly used carriers, matrices, and materials for immobilization of proteins, enzymes, nucleic acids, and living cells are briefly surveyed. Examples of delivery vehicles for multifunctional encapsulation of protein-like substances are presented. Particular attention is paid to prospects of using metal-organic frameworks in medicine and biotechnology.

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“…The use of nanosized formulations has been proven to be a promising strategy to alleviate some of these problems. Peptide and protein nanogels are particularly attractive as they allow to generate nanosized formulations of these biologics with essentially quantitative encapsulation efficiency. − …”

Section: Introductionmentioning

confidence: 99%

Reduction-Sensitive Protein Nanogels Enhance Uptake of Model and Tumor Lysate Antigens In Vitro by Mouse- and Human-Derived Dendritic Cells

Berti

Boarino

Graciotti

et al. 2021

ACS Appl. Bio Mater.

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Peptides and proteins represent an emerging class of powerful therapeutics. Peptide and protein nanogels are attractive carriers for the transport and delivery of biologically active peptides and proteins because they allow essentially quantitative encapsulation of these biologics. One interesting field of use of peptide and protein nanogels is the transport of antigens and adjuvants in cancer immunotherapy. This study demonstrates the use of reduction-sensitive protein nanogels for the delivery of ovalbumin and oxidized tumor lysate-based antigens to mouse and human-donor-derived dendritic cells. Challenging mouse-derived and human dendritic cells with reduction-sensitive ovalbumin nanogels was found to significantly enhance antigen uptake as compared to the use of the corresponding free protein antigen. The experiments with mouse-derived dendritic cells further showed that the administration of ovalbumin in the form of reduction-sensitive nanogels enhanced dendritic cell maturation as well as the presentation of the SIINFEKL epitope as compared to experiments that use free ovalbumin. In addition to ovalbumin as a model antigen, the feasibility of reduction-sensitive nanogels was also demonstrated for the delivery of oxidized, whole tumor lysate-based cancer antigens. In experiments with dendritic cells harvested from human donors, dendritic cell uptake of the oxidized tumor lysate antigen was significantly enhanced in experiments that used oxidized tumor lysate nanogels as compared to the free antigen.

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Long-term delivery of protein and peptide therapeutics for cancer therapies

Cited by 17 publications

References 138 publications

Topical Application of Peptide–Chondroitin Sulfate Nanoparticles Allows Efficient Photoprotection in Skin

Topical Application of Peptide–Chondroitin Sulfate Nanoparticles Allows Efficient Photoprotection in Skin

Methods of Encapsulation of Biomacromolecules and Living Cells. Prospects of Using Metal–Organic Frameworks

Reduction-Sensitive Protein Nanogels Enhance Uptake of Model and Tumor Lysate Antigens In Vitro by Mouse- and Human-Derived Dendritic Cells

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