Long non‐coding RNA‐H19 promotes ovarian cancer cell proliferation and migration via the microRNA‐140/Wnt1 axis

Abstract

To explore the effect and underlying molecular mechanism of long non-coding RNA (lncRNA)-H19 on ovarian cancer (OC) cells, a total of 41 cases of OC and adjacent normal tissues were collected. H19 and microRNA (miR)-140 expressions in OC tissues and cells were detected using quantitative real-time polymerase chain reaction (qRT-RCR). The correlation between H19 expression and prognosis of OC patient was analyzed. siRNA (si)-H19 and si-negative control (NC) were transfected into OC cells. Cell proliferation was checked by cell counting kit-8 assay and colony formation assay, and cell migration and invasion were analyzed via Transwell assay. The targeted binding relationship between H19 and miR-140 was predicted and verified, miR-140 downstream gene was predicted and Wnt1 was screened out. The impact of in-miR-140 on the si-H19-induced decreased OC cell proliferation and migration was evaluated. H19 expression was upregulated in OC tissues and cells, and its overexpression was associated with a poor prognosis of OC. si-H19 remarkably reduced OC cell proliferation and migration. H19 upregulated Wnt1 expression through targeting miR-140 in OC cells. Altogether, miR-140 was notably downregulated in OC, and in-miR-140 partially inhibited the si-H19-induced decrease of OC cell proliferation and migration. H19 competitively bound to miR-140 to upregulate Wnt1, thereby promoting OC cell proliferation and migration.

Long noncoding RNA H19 in ovarian biology and placenta development

“…57 To determine the role of H19 in ovarian cancer metastasis, different researchers manipulated H19 expression in epithelial ovarian cancer cells and then assessed the effects. In some of these studies, H19 overexpression was observed to promote the viability, proliferation, and invasion of ovarian cancer cells [58][59][60] by upregulating WNT1 through the downregulation of miR-140. 58 Consistently, H19 overexpression promoted ovarian cancer cell invasion by increasing the expression of TWIST1, SLUG, and SNAIL while reducing Ecadherin expression.…”

“…In some of these studies, H19 overexpression was observed to promote the viability, proliferation, and invasion of ovarian cancer cells [58][59][60] by upregulating WNT1 through the downregulation of miR-140. 58 Consistently, H19 overexpression promoted ovarian cancer cell invasion by increasing the expression of TWIST1, SLUG, and SNAIL while reducing Ecadherin expression. 59 Additionally, H19 overexpression resulted in the activation of the PI3K/AKT signaling pathway via the downregulation of miR-140-5p to promote the proliferation and invasion of ovarian cancer cells while reducing their apoptosis.…”

The role of long noncoding RNA H19 in gynecological pathologies: Insights into gene regulation and immune modulation (Review)

“…This pattern implies the pivotal role of lncRNA H19 in embryogenesis and fetal growth (27,28). Postnatal expression of lncRNA H19 diminishes significantly but maintains a residual level in select tissues, including breast, uterus, myocardium, skeletal muscle and the adrenal gland (29)(30)(31)(32)(33)(34). During tissue regeneration or tumorigenesis, lncRNA H19 function reactivates, participating in damage regulation and repair, or acting as either a tumor suppressor gene or an oncogene (35,36).…”

Epithelial-mesenchymal transition-related long noncoding RNAs in gastric carcinoma

“…Depletion of LINC00649 inhibits YAP1 and releases miR-16-5p, leading to the recovery of the Hippo pathway, and some downstream oncogenes are suppressed accordingly, such as EGFR, SOX2, and OCT4, suppressing the malignant phenotypes in GC cells ( Wang H. et al, 2021 ). YAP1 has been identified as an oncogene, which can promote the pathogenesis of multiple cancers and immunosuppression ( He S. et al, 2021 ; Wang and Gao, 2021 ). LncRNA metastasis associated lung adenocarcinoma transcript 1 (MALAT1) promotes cell proliferation and invasion in GC, and its up-regulation is associated with local invasion, lymph node metastasis, and TNM stage.…”

“…Z. et al, 2020 ). H19/miRNA-140 axis promotes ovarian cancer cell migration by upregulating Wnt1 expression ( Wang and Gao, 2021 ). Additionally, H19 upregulates PFTK1 expression through targeting miR-194 in pancreatic cancer cells ( Sun Y. et al, 2019 ).…”