Long-range surface plasmons for sensitive detection of bacterial analytes

Vala, Milan; Etheridge, Stacey M.; Roach, John A. G.; Homola, Jiřı́

doi:10.1016/j.snb.2008.08.029

Cited by 121 publications

(65 citation statements)

References 15 publications

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“…The specific response was only observed for Ab3, the differential signal shown in Figure 3 clearly indicates microbes captured by the antibody and only slowly dissociating from the formed immunocomplex. Regarding direct immunosensing of microbial cells, these presented data from Biacore generally correspond to our previous results obtained using the piezoelectric immunosensor [12]; directly operating biosensors could achieve sufficiently low detection limits for microbes only through signal enhancing steps and complex sensing strategies [16]. Thus, Ab3 was chosen for further experiments and construction of electrochemical immunosensors.…”

Section: Characterization Of Antibodiessupporting

confidence: 80%

Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols

et al. 2011

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The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 10 3 to 10 8 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.

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Section: Characterization Of Antibodiessupporting

confidence: 80%

Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols

et al. 2011

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“…By exploiting the very narrow dip in the LRSPR configuration and advanced photonic components Slavik and Homola [76] reported an SPR sensor with very low detection limit (2:5 ¢10 8 RIU). Within another experimental study, Vala and coworkers [77] reported an SPR sensor based on spectroscopy of LSPR for the detection of large analyte such as latex beads and bacteria. In the LSPR sensor a spectral sensitivity of 59 000 nm/RIU was measured, which is about 8-fold higher than the sensitivity of a conventional SPR sensor when the resonance was obtained close to 850 nm.…”

Section: Long-range Sprmentioning

confidence: 99%

Sensitivity‐enhancement methods for surface plasmon sensors

Shalabney

Abdulhalim

2011

Laser & Photonics Reviews

426

254

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Surface plasmon resonance (SPR) sensors have been a mature technology for more than two decades now, however, recent investigations show continuous enhancement of their sensitivity and their lower detection limit. Together with the recent investigations in localized SPR phenomena, extraordinary optical transmission through nanoapertures in metals, and surface-enhanced spectroscopies, drastic developments are expected to revolutionize the field of optical biosensing. Sensitivity-enhancement (SE) techniques are reviewed focusing both on the physical transduction mechanisms and the system performance. In the majority of cases the SE is associated with the enhancement of the electromagnetic field overlap integral describing the interaction energy within the analyte. Other important mechanisms are the interaction between plasmons and excitons and between the analyte molecules and the metal surface. The lower detection limit can be reduced significantly if systems with high signal-to-noise ratio are used such as common-path interferometry, ellipsometry or polarimetry systems.

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“…Both background index changes and surface binding will result in different shifts for the angle of excitation of the LR and SR SPRs. The LRSP has a longer penetration length in the surrounding medium, making it suitable for cell and bacterium detection [24]. By utilizing the special LRSP mode, the surface plasmon probe depth, and potentially the sensitivity to large analytes, can be increased significantly compared to conventional surface plasmon sensors.…”

Section: Spr Sensor Detection Sensitivity and Limitationsmentioning

confidence: 99%