Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5α in Bioaerosols

Abstract

The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 10 3 to 10 8 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.

Bioaerosol research: Yesterday, today and tomorrow

“…最新的研究表明, 利用微流控与LAMP可以实现空气中的病原体的检 测 [132] , 并且针对空气中低浓度病原体的快速检测, 研 发 了 大 流 量 快 速 生 物 气 溶 胶 采 样 器 , 其 切 割 点 为 2 μm, 每分钟可采集1000 L空气, 对于快速富集等起 到重要作用 [131] . 另一方面, 针对非可培养的生物组分, [133,134] [135] 、压电晶体生物传感器 [136] 、电化学生物 传感器 [137] 和硅纳米线场效应晶体管(SiNW-FET)生 物传感器 [138] 等. 硅纳米线传感器通过场效应晶体管 (FET)将分子结合瞬间转变成微电子信号, 由于其灵 敏度高、选择性好以及快速检测(电信号可以直接读 数)的优点, 已经被广泛用于检测化学物质、生物分 子、病毒等 [138,139] .…”

Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms

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Electrochemical immunosensor for Enterobacter sakazakii detection based on electrochemically reduced graphene oxide–gold nanoparticle/ionic liquid modified electrode

“…Electrochemical immunosensor has attracted much research interest because of its high sensitivity, fast response, low cost and uncomplicated instrumentation [8][9][10][11].…”