Long noncoding RNAs as a piece of polycystic ovary syndrome puzzle

Abolghasemi, Maryam; Mahjoub, S.

doi:10.1007/s11033-021-06196-1

Cited by 3 publications

(2 citation statements)

References 39 publications

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“…PGF 2α may also participate in the induction of CL cell membrane damage and CL cell apoptosis, leading to the degradation of the CL structure, which involves a variety of cytokines and immune functions [28]. Previous studies have shown that lncRNA SRA stimulates mouse granulosa cell growth, changes the cell cycle distribution with an increase in cyclins D1, E, and B, and inhibits cell apoptosis by upregulating Bcl2 and downregulating Bax [29,30]. However, our results showed that neither overexpression nor silencing of Hand2os1 affected the proliferation and apoptosis of LCs.…”

Section: Discussionmentioning

confidence: 99%

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

Jia

Liu

Gong

et al. 2022

Veterinary Sciences

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The corpus luteum plays a key role in pregnancy maintenance and estrous cycle regulation by secreting progesterone. Hand2os1 is an lncRNA located upstream of Hand2, with which a bidirectional promoter is shared and is involved in the regulation of cardiac development and embryo implantation in mice. The aim of this study was to investigate the expression and regulation of Hand2os1 in the ovaries. Here, we used RNAscope to detect differential expression of Hand2os1 in the ovaries of cycling and pregnant mice. Hand2os1 was specifically detected in luteal cells during the proestrus and estrus phases, showing its highest expression in the corpus luteum at estrus. Additionally, Hand2os1 was strongly expressed in the corpus luteum on day 4 of pregnancy, but the positive signal progressively disappeared after day 8, was detected again on day 18, and gradually decreased after delivery. Hand2os1 significantly promoted the synthesis of progesterone and the expression of StAR and Cyp11a1. The decreased progesterone levels caused by Hand2os1 interference were rescued by the overexpression of StAR. Our findings suggest that Hand2os1 may regulate the secretion of progesterone in the mouse corpus luteum by affecting the key rate-limiting enzyme StAR, which may have an impact on the maintenance of pregnancy.

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Section: Discussionmentioning

confidence: 99%

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

Jia

Liu

Gong

et al. 2022

Veterinary Sciences

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“…Changes in its activity are closely associated with cell growth, proliferation, differentiation, and apoptosis. An increasing number of studies have shown that lncRNAs are related to PCOS, playing a role in sex hormone secretion, IR, follicle development, GCs proliferation, and apoptosis, and participate in almost every link between the occurrence and development of PCOS [ 9 , 10 ]. Micro-RNAs (miRNAs) are another type of non-coding RNA, with 19–25 nucleotides in length.…”

Section: Introductionmentioning

confidence: 99%

LncRNA SNHG12 promotes cell proliferation and inhibits apoptosis of granulosa cells in polycystic ovarian syndrome by sponging miR-129 and miR-125b

Xuan,

Jin,

Zhou

et al. 2024

J Ovarian Res

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Background Polycystic ovarian syndrome (PCOS) is the most common endocrine disease in women of childbearing age which is often associated with abnormal proliferation or apoptosis of granulosa cells (GCs). Studies proved that long non-coding RNA SNHG12 (lncRNA SNHG12) is significantly increased in ovarian cancer and cervical cancer patients and cells. The inhibition of lncRNA SNHG12 restrains the proliferation, migration, and invasion in tumor cells. Objective This study explores the role of lncRNA SNHG12 in the apoptosis of GCs in PCOS and the underlying regulated mechanism. Methods In this study, the injection of dehydroepiandrosterone (DHEA) successfully induced the PCOS model in SD rats. The human granulosa-like tumor cell line KGN was incubated with insulin to assess the effects of lncRNA SNHG12 on GC proliferation and apoptosis. Results Overexpression of lncRNA SNHG12 influenced the body weight, ovary weight, gonadal hormone, and pathological changes, restrained the expressions of microRNA (miR)-129 and miR-125b, while downregulation of lncRNA SNHG12 exerted the opposite effects in PCOS rats. After silencing lncRNA SNHG12 in cells, the cell viability and proliferation were lessened whereas apoptosis of cells was increased. A loss-of-functions test was implemented by co-transfecting miR-129 and miR-125b inhibitors into lncRNA SNHG12-knocking down cells to analyze the effects on cell viability and apoptosis. Next, the existence of binding sites of SNHG12 and miR-129/miR-125b was proved based on the pull-down assay. Conclusion lncRNA SNHG12 might be a potential regulatory factor for the development of PCOS by sponging miR-129 and miR-125b in GCs.

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Would Non-coding RNA Resolve the Polycystic Ovary Syndrome (PCOS) Puzzle?

Alhamdan

2024

Obstetrics and Gynecology

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Polycystic ovary syndrome (PCOS) is the most common endocrine heterogeneous reproductive disorder. This metabolic disease affects around 5–10% of women and accounts for 75% of anovulatory infertility all over the world. The complexity of the disease as manifested by the involvement of multiple underlying mechanisms and the lack of specific and sensitive biomarkers, make it difficult to timely manage and treat the disease. Remarkably, genetic, epigenetics, and environmental variations may contribute considerably to the pathogenicity of PCOS. Recent investigations indicated that non-coding RNAs (ncRNA) were involved in the occurrence and development of PCOS. Thus, this chapter aimed to summarize the current knowledge around the expression and dysregulation of ncRNA in human PCOS.

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Long noncoding RNAs as a piece of polycystic ovary syndrome puzzle

Cited by 3 publications

References 39 publications

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

Hand2os1 Regulates the Secretion of Progesterone in Mice Corpus Luteum

LncRNA SNHG12 promotes cell proliferation and inhibits apoptosis of granulosa cells in polycystic ovarian syndrome by sponging miR-129 and miR-125b

Would Non-coding RNA Resolve the Polycystic Ovary Syndrome (PCOS) Puzzle?

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