Long noncoding RNA ZEB1-AS1 acts as a Sponge of miR-141-3p to Inhibit Cell Proliferation in Colorectal Cancer

Abstract

Evidence shows that long noncoding RNAs (lncRNAs) play key roles in various cancers, including colorectal cancer. In this current study, we found that the expression of ZEB1-AS1 in colorectal cancer tissues and cell lines was significantly upregulated, and positively correlated with advanced stage of colorectal cancer. Kaplan-Meier assays also indicated that the expression of ZEB1-AS1 was correlated with poor prognosis in patients with colorectal cancer. Knocking down of ZEB1-AS1 inhibited the proliferation of colorectal cancer cells. Subcellular fractionation analyses suggested that ZEB1-AS1 was majorly distributed in cytoplasm of SW480 and LOVO cells. Thus, ZEB1-AS1 might act as a competing endogenous RNA. MicroRNA array analysis suggested that miR-141-3p was significantly downregulated in CRC tissues, which was further verified by RT-qPCR. The results of luciferase reporter assay proved that miR-141-3p was a target of ZEB1-AS1. Functionally, miR-141-3p inhibitor reversed the anti-proliferation effect of sh-ZEB1-AS1 on colorectal cancer cells. Collectively, ZEB1-AS1 may contribute to colorectal cancer cell proliferation by sponging miR-141-3p.

The m7G-Related Long Noncoding RNA Signature Predicts Prognosis and Indicates Tumour Immune Infiltration in Colon Cancer

“…Siena confirmed that lncRNA ZEB1-AS1 can influence the invasiveness and phenotypic transformation of melanoma through epithelial to mesenchymal transition (EMT) ( Siena et al, 2019 ). Wu believes that ZEB1-AS1 may fuel the profitability of colorectal cancer cells by saponifying miR-141-3p ( Wu et al, 2020 ). LncRNA LINC00997 has been discovered to perform a regulatory role in tumours in recent years.…”

Long Non-Coding RNAs as Molecular Biomarkers in Cholangiocarcinoma

“…ZEB1-AS1 is an antisense transcript originating from the promoter of Zinc finger E-box binding homeobox 1(ZEB1), a prominent factor in the EMT process of tumor epithelial cells ( Li et al, 2016 ; Caramel et al, 2018 ). ZEB1-AS1 plays a carcinogenic factor in various digestive system tumors, for instance, hepatocellular carcinoma, esophageal cancer, colorectal cancer, and gastric cancer ( Li et al, 2016 ; Zhang et al, 2018b ; Zhao et al, 2019 ; Wu et al, 2020 ). Jiang et al (2020a) proved that ZEB1-AS1 was overexpressed in CCA and promoted CCA growth along with metastasis in vivo , as well as in vitro experiments.…”

MicroRNAs and ‘Sponging’ Competitive Endogenous RNAs Dysregulated in Colorectal Cancer: Potential as Noninvasive Biomarkers and Therapeutic Targets

“…Lowered miR-200a and elevated miR-200b in serum show similar patterns of being correlated with poor outcomes in CRC patients [89,211]. Moreover, the effectiveness of the carcinogenic influence of each member of the miR-200 family can potentially be mitigated by other ncRNAs that competitively inhibit their actions through sponging [217][218][219][220]. As in the case of other miRNAs and CRC, the basic presence or absence of miR-200 family transcripts in the biofluids of CRC patients alone is not enough to be diagnostic and must be considered within a cellular and histological framework while also considering expression levels of other contextually relevant and impactful ncRNAs.…”