Long Noncoding RNA GM16343 Promotes IL-36β to Regulate Tumor Microenvironment by CD8⁺T cells

Abstract

Objective: To investigate the effect of long noncoding RNA GM16343 on interleukin 36β promotion of CD8+T cells in tumor microenvironment regulation. Methods: The differentially expressed long noncoding RNA in interleukin 36β-stimulated mouse CD8+T cells was screened by gene chip technology, and the significant differentially expressed long noncoding RNAs were verified by real-time polymerase chain reaction. The lentiviral vector that overexpresses or knockdown GM16343 was constructed, transfected into CD8+T cells, and stimulated with interleukin 36β, and the amount of interferon γ secreted was detected by enzyme-linked immunosorbent assay. A mouse subcutaneous xenograft model that stably express interleukin 36β was established, and the tumor size and mouse survival time were observed by stimulation with CD8+T cells overexpression or knockdown of GM16343. Results: A total of 12 long noncoding RNAs with significant differences were screened by gene chip analysis. Real-time polymerase chain reaction showed that the difference in GM16343 was larger, and the difference between the groups was observed to be the most significant. Compared to control group, CD8+T cells overexpressing GM16343 increased the secretion of interferon γ, and the tumor diameter of the mice after stimulation showed significant reduction, and the survival time showed significant prolongation. Compared to control group, the CD8+T cells after GM16343 were knocked down. The interferon γ secretion was decreased, and no significant change in tumor diameter and survival time was observed. Conclusion: Interleukin 36β may enhance antitumor immune response of CD8+T cells by regulating GM16343.

Immune-Related Four-lncRNA Signature for Patients with Cervical Cancer

“…For instance, the downregulated lncRNA AGER has been demonstrated to be closely related to T cell status in lung cancer [ 10 ]. lncRNA GM16343 is regulated by interleukin 36 β to strengthen the antitumor immune response of CD8 + T cells [ 11 ]. lncRNA LINK-A has been demonstrated to downregulate antigen presentation and intrinsic suppression in triple-negative breast cancer [ 12 ].…”

LINC01355 Contributes to Malignant Phenotype of Oral Squamous Cell Carcinoma and Cytotoxic T Cell Infiltration via Activating Notch Signaling Pathway

“…For instance, Lnc-Tim3 can exacerbate CD8+ T cell exhaustion through binding to Tim-3 and triggering nuclear translocation of Bat3 in liver cancer [ 26 ]. LncRNA GM16343 can induce IL-36 β to modulate tumor microenvironment via CD8+ T cells [ 27 ]. LINC00301 can facilitate lung cancer progression and triggers an immune-suppressing microenvironment through regulating the HIF1 α [ 28 ].…”

Long noncoding RNA: a dazzling dancer in tumor immune microenvironment

“…The research on mice found that difference of lncRNA-GM16343 was the most obvious between the groups by taking advantage of gene chip technology in mouse CD8 + T cells stimulated by IL-36β [ 102 ]. GM16343 stimulated the secretion of IFN-γ in CD8 + T cells, markedly reduced tumor volume of mice, and potently prolonged the survival time.…”

“…GM16343 stimulated the secretion of IFN-γ in CD8 + T cells, markedly reduced tumor volume of mice, and potently prolonged the survival time. It was indicated GM16343 profoundly dampened tumor growth through affecting antitumor immune function of CD8 + T cells (Table 1 ) [ 102 ]. Intriguingly, a recent study conducted by Wang et al demonstrated that exosomes originated from exhausted CD8 + T cells could be internalized by non-exhausted CD8 + T cells, and impeding proliferation capacity and the secretion of cytokines, i.e.…”