Long noncoding RNA ENST00000413528 sponges microRNA‐593‐5p to modulate human glioma growth via polo‐like kinase 1

Abstract

Summary Aims In this study, we examined the expression of lncRNA ENST00000413528 in glioma and determined its role in glioma development. Methods LncRNA ENST00000413528 was detected in glioma tissues by lncRNA microarray. Then, we performed real‐time PCR, CCK‐8, colony formation assay, flow cytometry, caspase‐3/7 assay and animal experiment to detect the function of ENST00000413528 in glioma after ENST00000413528 knockdown. Subsequent bioinformatics analysis, luciferase reporter assays and RNA immunoprecipitation (RIP) assay western blotting indicated possible downstream regulatory molecules. The expression of PLK1 in glioma tissues was also examined by immunohistochemistry staining. Results Expression of ENST00000413528 was significantly increased in glioma tissues and LN229 and U251 cells. PLK1 protein could not be detected in peritumoral brain edema (PTBE) tissues; however, it showed an increasing number of positively cytoplasmic stained from WHO‐Grade II to Grade III gliomas. Knockdown of ENST00000413528 in glioma cells inhibited cell proliferation and colony formation abilities, induced the G0/G1 arrest of the cell cycle, and promoted apoptosis. The dual reporter assay and RNA immunoprecipitation assay verified the interaction between ENST00000413528 and miR‐593. We also demonstrated that polo‐like kinase 1 (PLK1) was regulated by miR‐593; PLK1 messenger RNA lacking 3’UTR partially reversed the effects caused by ENST00000413528 knockdown or miR‐593 upregulation. Conclusion lncRNA ENST00000413528 is closely related to the development of glioma via the miR‐593‐5p/PLK1 pathway.

Analysis of the Expression of Cell Division Cycle-Associated Genes and Its Prognostic Significance in Human Lung Carcinoma: A Review of the Literature Databases

“…Previous studies show that the Polo-like kinase 1(PLK1) is highly expressed in LC, which predicts the poor survival in metastatic LC patients [56,57]. In addition, the PLK1 pathway plays a certain role in the progression of HCC [58], glioma [59], and lung adenocarcinoma [60]. e current research systemically examines the expression of CDCA genes and its prognostic significance in LC, which sheds more light on the complexity and heterogeneity of LC biological properties at the molecular level.…”

Bioinformatics and system biology approach to identify the influences among COVID-19, influenza, and HIV on the regulation of gene expression

“…GSEA analysis of all genes in the three datasets revealed specific pathway enrichments. The COVID-19 dataset showed enrichment in the PLK1 pathway, associated with stem cell cancer ( 50 ), glioma ( 51 ), and lung adenocarcinoma ( 52 ). The influenza dataset exhibited enrichment in the oxidative stress response signaling pathway, a potential therapeutic target for gastric cancer ( 53 ).…”

Circular RNA ZNF609 promotes the malignant progression of glioma by regulating miR-1224-3p/PLK1 signaling

“…PLK1 has been verified to be involved in various cancers, such as hepatocellular carcinoma 40 , breast cancer 41 , prostate cancer 42 , acute myeloid leukemia 43 . Additionally, PLK1 has been reported to be upregulated in glioma and can markedly promote cell proliferation and migration 44 - 46 . Moreover, we demonstrated that circ-ZNF609 could promote the expression of PLK1 via sponge miR-1224-3p.…”