Long Noncoding RNA DANCR Is a Positive Regulator of Proliferation and Chondrogenic Differentiation in Human Synovium-Derived Stem Cells

Zhang, Lei; Yang, Chao; Chen, Shuo; Wang, Guihua; Shi, Benwei; Tao, Xin; Zhou, Lei; Zhao, Jianning

doi:10.1089/dna.2016.3544

Cited by 54 publications

(47 citation statements)

References 28 publications

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“…These results present evidence that DANCR acts as an endogenous sponge of miR‐496 and that DANCR and miR‐496 negatively regulate each other in ADC. DANCR has been reported to function via suppressing protein expression or interacting directly with mRNA, but our results provide evidence of a novel mechanism of action for DANCR .…”

Section: Discussionmentioning

confidence: 56%

LncRNA‐DANCR contributes to lung adenocarcinoma progression by sponging miR‐496 to modulate mTOR expression

Rui

Guo

et al. 2017

J Cellular Molecular Medi

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Long non‐coding RNAs (lncRNAs) have emerged as new and important regulators of pathological processes including tumour development. In this study, we demonstrated that differentiation antagonizing non‐protein coding RNA (DANCR) was up‐regulated in lung adenocarcinoma (ADC) and that the knockdown of DANCR inhibited tumour cell proliferation, migration and invasion and restored cell apoptosis rescued; cotransfection with a miR‐496 inhibitor reversed these effects. Luciferase reporter assays showed that miR‐496 directly modulated DANCR; additionally, we used RNA‐binding protein immunoprecipitation (RIP) and RNA pull‐down assays to further confirm that the suppression of DANCR by miR‐496 was RISC‐dependent. Our study also indicated that mTOR was a target of miR‐496 and that DANCR could modulate the expression levels of mTOR by working as a competing endogenous RNA (ceRNA). Furthermore, the knockdown of DANCR reduced tumour volumes in vivo compared with those of the control group. In conclusion, this study showed that DANCR might be an oncogenic lncRNA that regulates mTOR expression through directly binding to miR‐496. DANCR may be regarded as a biomarker or therapeutic target for ADC.

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Section: Discussionmentioning

confidence: 56%

LncRNA‐DANCR contributes to lung adenocarcinoma progression by sponging miR‐496 to modulate mTOR expression

Rui

Guo

et al. 2017

J Cellular Molecular Medi

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“…SMSCs have shown promising efficiency for cell-based regeneration of damaged articular cartilage [14]. Our previous data demonstrated that chondrogenic differentiation of SMSCs was promoted by lncRNA DANCR [12,13]. In the present study, we characterized the underlying molecular mechanism that overexpression of DANCR down-regulates miR-1305 .…”

Section: Discussionmentioning

confidence: 77%

“…Dysfunction of lncRNA has been observed in a variety of human diseases [19–21]. Our previous data demonstrated that lncRNA DANCR, which was first identified in hepatocellular carcinoma (HCC) [22], promoted the cell proliferation and chondrogenic differentiation through up-regulating the expression of Smad3 and STAT3 [12]. These results provided one of the mechanisms for the role of SMSC in cartilage repair.…”

Section: Introductionmentioning

confidence: 99%

“…It is documented that MSCs directly act in cartilage formation, as well as release trophic factors, and promote angiogenesis [9–11]. Amongst the various available sources, MSCs seem to have many advantages over their counterparts, while recent study demonstrated that synovium-derived MSCs (SMSCs), which have better chondrogenic potential compared with MSC, are gaining momentum [12–17]. Improved MRI features, histology, and better clinical outcome have been achieved in cartilage repair derived from SMSCs [14].…”

Section: Introductionmentioning

confidence: 99%

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Long noncoding RNA DANCR regulates miR-1305-Smad 4 axis to promote chondrogenic differentiation of human synovium-derived mesenchymal stem cells

et al. 2017

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miRNAs have been reported to regulate cellular differentiation by modulating multiple signaling pathways. Long noncoding RNA (lnc RNA) DANCR was previously identified to be critical for the chondrogenesis of human synovium-derived mesenchymal stem cells (SMSC), however, the underlying molecular mechanism requires better understanding. Here, miRNA expression profiling in DANCR overexpressed in SMSCs identified significant down-regulation of miR-1305, which serves as a downstream target of DANCR. Notably, miR-1305 overexpression reversed DANCR-induced cell proliferation and chondrogenic differentiation of SMSCs, which suggested that miR-1305 antagonized the function of DANCR. Mechanistically, highly expressed miR-1305 resulted in the decreased expression of the TGF-β pathway member Smad4, and inhibition of miR-1305 enhanced the expression level of Smad4. Depletion of Smad4 suppressed the promotion of DANCR in cell proliferation and chondrogenesis of SMSCs. Collectively, our results characterized miR-1305-Smad4 axis as a major downstream functional mechanism of lncRNA DANCR in promoting the chondrogenesis in SMSCs.

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“…DANCR, also termed ANCR, was initially identified as a non-coding RNA required to enforce the undifferentiated cell state within the epidermis (33)(34)(35). Currently, increasing evidence indicates that DANCR is involved in multiple biological processes, including stem cell differentiation, cell proliferation and cancer progression (13,16,29,36). Wang et al reported that DANCR promoted the proliferation, migration and invasion of NSCLC cell lines, SPC-A1 and H1299, via regulation of the tumor suppressor miR-758-3p (37).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA DANCR promotes HMGA2‑mediated invasion in lung adenocarcinoma cells

Jiang

2018

Oncol Rep

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Long non-coding RNAs (lncRNAs) have been reported to be key regulators in various types of cancer, including lung adenocarcinoma (LAD). The roles of the lncRNA differentiation antagonizing non-protein coding RNA (DANCR) and high mobility group AT-hook 2 (HMGA2) in LAD remain unclear. In the present study, it was revealed that the lncRNA DANCR was upregulated in LAD tissue and cell lines, compared with para-tumor tissue and a normal lung cell line. Additionally, elevated DANCR expression was associated with poor prognosis in the patients with LAD. Functionally, the study revealed that knockdown of DANCR inhibited invasion and HMGA2 expression in the LAD cell lines, SPCA1 and A549. Furthermore, HMGA2 was overexpressed in LAD tissue and in SPCA1 and A549 cells, compared with para-tumor tissue and a normal lung cell line. Inhibition of HMGA2 suppressed the invasive ability of SPCA1 and A549 cells, and DANCR promoted the invasive ability via regulation of HMGA2 in SPCA1 and A549 cells. The findings of the present study revealed that DANCR promoted the invasion of LAD cells by positively regulating HMGA2. Thus, a DANCR/HMGA2 axis may be a novel potential target in the molecular treatment of LAD.

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Long Noncoding RNA DANCR Is a Positive Regulator of Proliferation and Chondrogenic Differentiation in Human Synovium-Derived Stem Cells

Cited by 54 publications

References 28 publications

LncRNA‐DANCR contributes to lung adenocarcinoma progression by sponging miR‐496 to modulate mTOR expression

LncRNA‐DANCR contributes to lung adenocarcinoma progression by sponging miR‐496 to modulate mTOR expression

Long noncoding RNA DANCR regulates miR-1305-Smad 4 axis to promote chondrogenic differentiation of human synovium-derived mesenchymal stem cells

Long non‑coding RNA DANCR promotes HMGA2‑mediated invasion in lung adenocarcinoma cells

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