Long noncoding RNA DANCR regulates <i>miR-1305</i>-Smad 4 axis to promote chondrogenic differentiation of human synovium-derived mesenchymal stem cells

Zhang, Lei; Sun, Xiangyi; Chen, Shuo; Yang, Chao; Shi, Benwei; Zhou, Lei; Zhao, Jianning

doi:10.1042/bsr20170347

Cited by 47 publications

(30 citation statements)

References 40 publications

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“…Tong et al [ 4 ] pointed out that DANCR might a biomarker for osteoporosis. Moreover, DANCR has been reported to regulate chondrogenic differentiation [ 8 , 10 , 24 ]. The present study found an increased expression of lncRNA DANCR in periprosthetic wear particles and surrounding tissues, which indicated that DANCR might be related to OD.…”

Section: Discussionmentioning

confidence: 99%

LncRNA DANCR involved osteolysis after total hip arthroplasty by regulating FOXO1 expression to inhibit osteoblast differentiation

2018

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BackgroundAseptic loosening of artificial hip joint is a major complication affecting the long-term use of the artificial hip joint, and is the main cause of joint replacement failure. However, the mechanism of aseptic loosening of THR has not yet cleared. The aim of this study was to investigate the underlying mechanism of DANCR in osteoblast differentiation (OD).MethodsWe detected the expressions of DANCR and FOXO1 in clinical samples and mesenchymal stem cells (MSCs) by qRT-PCR and western blotting. The effects of polymethylmethacrylate (PMMA) on OD of MSCs were examined by alkaline phosphatase (ALP) activity and Alizarin Red S (ARS) staining. The expressions of OD markers were measured by qRT-PCR and western blotting. The mechanism of DANCR in OD was detected by RNA pull-down, RNA immunoprecipitation (RIP) assay and ubiquitination assays.ResultsCompared with the surrounding normal tissues, DANCR expression was up-regulated and FOXO1 expression was down-regulated in periprosthetic tissues. PMMA suppressed ALP activity, increased DANCR expression, and decreased the expressions of FOXO1, Runx2, Osterix (Ostx) and osteocalcin (OCN). ARS staining showed that PMMA inhibited the OD of MSCs. Knockdown of DANCR attenuated the inhibitory effect of PMMA on OD. Knockdown of FOXO1 could reverse the effect of si-DANC. RNA pull-down and RIP assay implicated that DANCR bound to FOXO1. Ubiquitination assay indicated that si-DANCR could repress Skp2-mediated ubiquitination of FOXO1.ConclusionLncRNA DANCR could inhibit OD by regulating FOXO1 expression.

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Section: Discussionmentioning

confidence: 99%

LncRNA DANCR involved osteolysis after total hip arthroplasty by regulating FOXO1 expression to inhibit osteoblast differentiation

2018

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“…Recently, growing investigations on transcriptome sequencing have evealed thousands of differentially expressed lncRNAs in various diseases, which may play roles in the development and progression of diseases [ 11 – 13 ]. However, studies focusing on the lncRNAs related to CS-induced airway inflammation is limited.…”

Section: Discussionmentioning

confidence: 99%

Microarray analysis of lung long non-coding RNAs in cigarette smoke-exposed mouse model

Wang

Chen

et al. 2017

Oncotarget

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Several studies have demonstrated the function of long nonâ€‘coding RNAs (lncRNAs) in various biological processes, yet their role underlying the susceptibility to cigarette smoke (CS)-induced airway inflammation remains limited. In the present study, we aimed to profile the expression of lncRNAs and mRNAs in CS-exposed mice. C57BL/6 mice were assigned into a single cigarette-smoking machine with or without CS exposure for 4 weeks, followed by lung tissue harvest and RNA isolation. Microarray analysis identified 108 lncRNAs and 119 mRNAs with differential expression levels in CS-exposed mouse lung tissue compared with those in control mice. The expression patterns of several lncRNAs were further confirmed by qRT-PCR. GO and pathway analyses showed that the altered mRNAs were mainly related to the processes of immune response, defense response and cell chemotaxis, cytokine-cytokine receptor interaction and chemokine signaling pathway. Moreover, a single lncRNA may co-expressed with several mRNAs, and so was the mRNA. Our findings uncovered the expression profile of lncRNAs and mRNAs in the lungs of CS-exposed mice, which may offer new insights into pathogenesis of CS-associated airway inflammatory disorders.

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“…DANCR activates SMSC differentiation into chondrocytes by increasing Smad3 [ 69 ]. Zhang et al found DANCR promoted chondrogenesis by downregulating the expression of miR-1305 which resulted in the decreased expression of Smad4 and activation of the TGF- β pathway in human synovium-derived stem cells (SMSCs) [ 70 , 71 ] ( Table 2 ).…”

Section: Lncrnas Involved In Chondrogenic Differentiationmentioning

confidence: 99%

An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells

Peng

Cao

et al. 2018

Stem Cells International

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Bone regeneration is very important for the recovery of some diseases including osteoporosis and bone fracture trauma. It is a multiple-step- and multiple-gene-involved complex process, including the matrix secretion and calcium mineralization by osteoblasts differentiated from mesenchymal stem cells (MSCs) and the absorption of calcium and phosphorus by osteoclasts differentiated from hematopoietic stem cells. Long noncoding RNAs (lncRNAs) are a family of transcripts longer than 200 nt without or with very low protein-coding potential. Recent studies have demonstrated that lncRNAs are widely involved in the regulation of lineage commitment and differentiation of stem cells through multiple mechanisms. In this review, we will summarize the roles and molecular mechanism of lncRNAs including H19, MALAT1, MODR, HOTAIR, DANCR, MEG3, HoxA-AS3, and MIAT in osteogenesis ossification; lncRNA ZBED3-AS1 and CTA-941F9.9, DANCR, and HIT in chondrogenic differentiation; and lncRNA DANCR in osteoclast differentiation. These findings will facilitate the development and application of novel molecular drugs which regulate the balance of bone formation and absorption.

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Long noncoding RNA DANCR regulates miR-1305-Smad 4 axis to promote chondrogenic differentiation of human synovium-derived mesenchymal stem cells

Cited by 47 publications

References 40 publications

LncRNA DANCR involved osteolysis after total hip arthroplasty by regulating FOXO1 expression to inhibit osteoblast differentiation

LncRNA DANCR involved osteolysis after total hip arthroplasty by regulating FOXO1 expression to inhibit osteoblast differentiation

Microarray analysis of lung long non-coding RNAs in cigarette smoke-exposed mouse model

An Overview of Long Noncoding RNAs Involved in Bone Regeneration from Mesenchymal Stem Cells

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