Long non-coding RNAs in the gastric juice of gastric cancer patients

Virgilio, Edoardo; Giarnieri, Enrico; Giovagnoli, Maria Rosaria; Montagnini, Monica; Proietti, A.; D’Urso, Rosaria; Mercantini, Paolo; Balducci, Genoveffa; Cavallini, Marco

doi:10.1016/j.prp.2018.07.023

Cited by 28 publications

(23 citation statements)

References 31 publications

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“…LncRNA refers to a non-coding RNA of base compositions ranging from 200 nt to 100000 nt NA, and it plays an important role in the biological processes such as cell cycle regulation, epigenetic inheritance regulation, transcription regulation and post-transcription regulation etc., and it is involved in a series of diseases in nervous system, cardiovascular system and malignant tumor etc.. [13][14][15][16] With the deepening of research, it is found that, more and more LncRNA are closely related to the generation and development of brain glioma. [17,18] LINC00152 refers to a member of LncRNA family, and it has abnormal expression at malignant tumors such as liver cancer, gastric cancer and colon cancer etc.. [19,20] The latest clinical studies have shown that, LINC00152 is associated with poor clinical prognosis of brain glioma. It inhibits the growth of glioma cells in the subcutaneously transplanted tumors in the nude mice by down-regulating LINC00152 expression.…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00152 promotes glioma cell proliferation via Src-YAP signaling pathway

Liang¹,

Lan²,

Jin³

et al. 2020

Preprint

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Objective The study is designed to observe the influence of LncRNA LINC00152 on the proliferation of glioma cells and explore the role of Src-YAP signal pathway in the process.Methods U251 and U87 cell were used for in vitro experiments and xenograft studies; transfection method was adopted to build a LINC00152 overexpressing cell strain, and cell viability was determined by MTT assay; cell colony formation ability was measured through colony formation assay, and protein expression was determined by Western blot; YAP protein expression distribution is detected through Immunofluorescence.Key findings LINC00152 overexpressing can enhance the U251 and U87 cell proliferation, the colony formation and the growing ability of subcutaneously transplanted tumor, and it induces YAP nuclear import and down-regulates p-LATS1 and p-YAP protein expression, and up-regulates p-Src protein expression. Src inhibitor 1 can inhibit the changes in protein expression and the cell colony formation of p-LATS1, p-YAP and p-Src, which are induced by overexpression of LINC00152 in U251 and U87 cells.Conclusions LINC00152 promotes cell proliferation of glioma U251 and U87, and the action might be associated with process of activation of Src, inhibition of phosphorylation cascade reaction of LATS1-YAP pathway and final inducing of YAP nuclear import.

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNA LINC00152 promotes glioma cell proliferation via Src-YAP signaling pathway

Liang¹,

Lan²,

Jin³

et al. 2020

Preprint

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“…Table 1 illustrates basic characteristics of the patient and control groups. All the specimens were collected and pretreated according to the previously described protocol and preserved at -80°C until RNA extraction [27].…”

Section: Sample Collectionmentioning

confidence: 99%

Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

Song

Zhong

et al. 2020

Preprint

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Background : The present study attempted to detect a specific circular RNA (circRNA) for the early diagnosis of gastric cancer (GC). Methods: We selected four patients with GC for this study. The total RNA of the malignant and adjacent tissues was extracted, and the circRNA was screened. The eight most upregulated and downregulated circRNAs with a statistically significant relation to GC and paired adjacent nontumorous tissues were identified using real-time fluorescent quantitative polymerase chain reaction (PCR). CircRNA expression was identified by quantitative reverse transcriptase PCR in 78 cases of GC and adjacent tissues, and in the gastric fluids of 30 patients with chronic nonatrophic gastritis, 30 patients with chronic atrophic gastritis, 21 with early GC, and 57 with advanced GC. Results: A total of 445 circRNAs, including 69 upregulated and 376 downregulated circRNAs, showed significant aberrant expression in GC tissues. A majority of the differentially expressed circRNAs originated from chr1, chr3, chr4, chr6, and chr11. Hsa_circ_000780 was significantly downregulated in 80.77% of GC tissues, and its level in GC tissues correlated with the tumor size, tumor stage, T stage, venous invasion, carcinoembryonic antigen, and carbohydrate antigen 19-9 expression. A crucial observation was the presence of this circRNA in the gastric fluid of patients with early and advanced GC. Conclusions: The present study uncovered a new hsa_circRNA expression profile in human GC, of which hsa_circ_000780 was significantly downregulated in GC tissues and in gastric fluid. It can be potentially used as a novel biomarker for early GC screening.

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“…14 Gastric juice is one of the commonly used materials and has significant advantage in the diagnosis of GC. 11,12,15,16,33 Guo et al explored that some circRNAs in GJ had the potential to be employed as novel indicators for the detection of high-risk gastric cancer patients. 12 TA B L E 3 Univariate and multivariate analysis for overall survival circ_0065149 expression showed an authentic role in GC oncogenesis, and its existence in exosomes was an biomarker for early screening and prognostic prediction for GC patients.…”

Section: Accumulating Literatures Showed That Alternation Of Non-codingmentioning

confidence: 99%

“…15 Virgilio et al indicated that lncRNAs, like H19, UCA1, ABHD11-AS1, and so forth, were supposed to be the prominent role in GC research, and emerged to be novel potential markers for GC diagnosis. 16,33 Thus, new biomarker in GJ showed a more distinguished advancement than traditional methods.…”

Section: Accumulating Literatures Showed That Alternation Of Non-codingmentioning

confidence: 99%

“…[10][11][12] Prominently, piRNAs have been disclosed to be present in a stable form in human body fluids, including the plasma and serum. [13][14][15][16] Moreover, Qu et al suggested that serum piRNAs expression could be used to be biomarkers for CRC detection and to predict prognosis. 14 Based on these studies, we deduced that detection of piRNAs in GJ might provide a non-invasive tool for diagnosis and prognosis for GC.…”

Section: Introductionmentioning

confidence: 99%

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Gastric juice piR‐1245: A promising prognostic biomarker for gastric cancer

Zhou

Liu

Meng

et al. 2019

Clinical Laboratory Analysis

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Background: Emerging reports demonstrated that PIWI-interacting RNAs (piR-NAs) played an indispensable role in tumorigenesis. However, it still remains elusive whether piR-1245 in gastric juice specific in stomach could be employed as a biomarker for gastric cancer (GC). The present work is aiming at exploring the possibility of piR-1245 in gastric juice as a potential marker to judge for diagnosis and prognosis of gastric cancer. Methods:Gastric juice was collected from 66 GC patients and 66 healthy individuals.Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was employed to measure the levels of piR-1245 expression. Then, the pattern of piR-1245 expression in gastric juice was determined between GC patients and healthy individuals. A receiver operating characteristic (ROC) curve was constructed for distinguishing GC from healthy individuals.Results: Gastric juice piR-1245 levels in GC were higher than those of controls (P < .0001). The value of area under ROC (AUC) was 0.885 (sensitivity, 90.9%; specificity, 74.2%; 95% confidence interval, 0.8286 to 0.9414). High gastric juice piR-1245 expression was signally correlated with tumor size (P = .013) and TNM stage (P = .001). GC patients with high piR-1245 expression in gastric juice exerted a poorer overall survival (OS) (P = .0152) and progression-free survival (PFS) (P = .013). COX regression analysis verified that gastric juice piR-1245 expression was an independent prognostic risk variable for OS (P < .05). Conclusions:The current study suggested that piR-1245 in gastric juice had the potential to be a useful biomarker for GC detection and prognosis prediction. K E Y W O R D Sbiomarker, gastric cancer, gastric juice, piR-1245, PIWI-interacting RNA

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Long non-coding RNAs in the gastric juice of gastric cancer patients

Cited by 28 publications

References 31 publications

Long noncoding RNA LINC00152 promotes glioma cell proliferation via Src-YAP signaling pathway

Long noncoding RNA LINC00152 promotes glioma cell proliferation via Src-YAP signaling pathway

Circular RNA hsa_circ_000780 has been identified by genomic expression profile analysis as a potential diagnostic marker for gastric cancer

Gastric juice piR‐1245: A promising prognostic biomarker for gastric cancer

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