Long Non-coding RNAs Genes Polymorphisms and Their Expression Levels in Patients With Rheumatoid Arthritis

Abstract

Long non-coding RNAs (lncRNAs) are increasingly recognized to play important roles in multiple autoimmune diseases. This study aimed to evaluate the association of four lncRNAs (ANRIL, lnc-DC, MALAT1, ZFAS1) genes single nucleotide polymorphisms (SNPs) with susceptibility to rheumatoid arthritis (RA) patients, as well as their expression levels. Seventeen SNPs of the four lncRNAs were genotyped in a cohort of 660 RA patients and 710 controls using improved multiple ligase detection reaction (iMLDR). The lncRNAs expressions in peripheral blood mononuclear cells (PBMCs) from 120 RA patients and 120 controls were detected by qRT-PCR. No significant differences were found for the allele and genotype frequencies distribution of ANRIL SNPs (rs1412830, rs944796, rs61271866, rs2518723, rs3217992), lnc-DC SNPs (rs7217280, rs10515177), MALAT1 SNPs (rs619586, rs4102217, rs591291, rs11227209, rs35138901), ZFAS1 SNPs (rs237742, rs73116127, rs6125607, rs6125608) between RA patients and normal controls (all P > 0.05). The genotype effects of dominant and recessive models were also evaluated, but no significant association was found. In addition, our results demonstrated that the rs944796 G allele, rs2518723 T allele, rs3217992 T allele frequencies were significantly associated with anti-CCP in RA patients (all P < 0.05). The haplotype CGTA frequency for ZFAS1 was significantly higher in RA patients (P = 0.036). Compared with normal controls, the expression levels of ANRIL, lnc-DC, MALAT1, ZFAS1 in PBMCs were significantly reduced in RA patients (all P < 0.001). Moreover, ZFAS1 expression was negatively associated with CRP in RA patients (P = 0.002). In summary, ANRIL, lnc-DC, MALAT1, and ZFAS1 genes SNPs were not associated with RA susceptibility, while altered ANRIL, lnc-DC, MALAT1, ZFAS1 levels in RA patients suggested that these lncRNAs might play a role in RA.

A genetic variant in the promoter of lncRNA MALAT1 is related to susceptibility of ischemic stroke

“…Zhang et al indicated rs4102217 and rs591291 SNPs were not associated with susceptibility of rheumatoid arthritis [37]. The Study of association with HCC have shown rs4102217 had a 1.32-fold risk in the dominant model, and rs591291 highlighted better prognosis in female and HBV negative subgroups, but association between MALAT1 haplotype (rs4102217-rs591291-rs11227209-rs619586) and HCC risk were not observed [23].…”

A genetic variant in the promoter of lncRNA MALAT1 is related to susceptibility of ischemic stroke

“…To our knowledge, the study of rs600231 A>G variant with risk of diseases have been rarely reported, but rs4102217 and rs591291 SNPs were evaluated in diseases. Zhang et al indicated rs4102217 and rs591291 SNPs were not associated with susceptibility of rheumatoid arthritis [37]. The Study of association with HCC have shown rs4102217 had a 1.32-fold risk in the dominant model, and rs591291 highlighted better prognosis in female and HBV negative subgroups, but association between MALAT1 haplotype (rs4102217-rs591291-rs11227209-rs619586) and HCC risk were not observed [23].…”

A genetic variant in the promoter of lncRNA MALAT1 is related to susceptibility of ischemic stroke

“…At present, studies on rs600231 A > G variant with disease have not been reported, but rs4102217 and rs591291 SNPs were evaluated in rheumatoid arthritis (RA) and HCC. Zhang et al indicated rs4102217 and rs591291 SNPs were not associated with RA susceptibility [35]. Studies of association with HCC have shown rs4102217 had a 1.32-fold risk in the dominant model, and rs591291 highlighted better prognosis in female and HBV negative subgroups, but association between MALAT1 haplotype (rs4102217-rs591291-rs11227209-rs619586) and HCC risk were not found [22].…”