Long Non-Coding RNA TRG-AS1 Promoted Proliferation and Invasion of Lung Cancer Cells Through the miR-224-5p/SMAD4 Axis

Zhang, Mengyan; Zhu, Weiguo; Haeryfar, Mansour; Jiang, Sumei; Jiang, Xiang; Chen, Wei; Li, Jiancheng

doi:10.2147/ott.s297336

Cited by 11 publications

(6 citation statements)

References 23 publications

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“…Previous study found that lncRNA SNHG12 promotes the cell proliferation, invasion and epithelial-mesenchymal transition of pancreatic cancer cells by absorbing miRNA-320b [ 35 ]. Li et al found that lncRNA TRG-AS1 facilitate NSCLC cell proliferation and invasion by the miR-224-5p/SMAD4 axis [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Next-generation sequencing identifies HOXA6 as a novel oncogenic gene in low grade glioma

Jiang

Wang

Guo

et al. 2022

Aging

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Background: Low grade glioma is one of the most common lethal cancers in the human nervous system. Emerging evidence has demonstrated that homeobox A cluster (HOXA) gene family plays a critical role in the transcriptional regulation as well as cancer initiation and progression. However, the expression, biological functions and upstream regulatory mechanism of 11 HOXAs in low grade glioma are not yet clear. Methods: In this study, we utilized various public databases and bioinformatics analyzed, including TCGA, CGGA, Rembrandt, HPA, LinkedOmics, cBioPortal, TISDIB, single-sample GSEA (ssGSEA), TIMER, LnCeVar, LASSO regression, Cox regression, Kaplan-Meier plot, and receiver operating, characteristic (ROC) analyses, GDSC and CTRP databases to analyzed the mRNA and protein expression profiles, gene mutation, clinical features, diagnosis, prognosis, signaling pathway, TMB, immune subtype, immune cell infiltration, immune modulator, ceRNA network and drug sensitivity of 11 HOXAs. Growth curve and transwell assays were utilized to study the biological characteristics of HOXA6 in LGG progression. Results: In the present study, we found that 11 HOXAs (HOXA1, HOXA2, HOXA3, HOXA4, HOXA5, HOXA6, HOXA7, HOXA9, HOXA10, HOXA11 and HOXA3) were consistently up-regulated in LGG tissues and GBM tissues. Up-regulated of the HOXAs expression were significantly correlated with higher tumor stage, IDH mutation status, 1p/19q co-deletion, histological type and primary therapy outcome. Survival analyses showed that higher expression of HOXA1, HOXA2, HOXA3, HOXA4, HOXA5, HOXA7, HOXA9, HOXA10, HOXA11 and HOXA13 were correlated with shorter overall survival (OS), disease-specific survival (DSS) and progression-free survival (PFS) in LGG patients. Univariate and multivariate analyses revealed that HOXA1, HOXA6 expression and tumor grade, age, primary therapy outcome and age were independent factors affecting the prognosis of LGG patients. ROC curve analysis of HOXAs showed that HOXAs had a high accuracy (AUC > 0.80) in predicting LGG. Furthermore, gene functional enrichment analysis indicated that HOXAs mainly involved in the inflammatory response and immune regulation signaling pathway. CNV and DNA methylation significantly affect the expression of HOXAs. Finally, we uncover that HOXAs expression are highly correlated with immune cells infiltrate, immune modulator and drug sensitivity. We also uncover that the HOXAs related ceRNA network in LGG. More importantly, we found that HOXA6 was highly expressed in LGG cells lines and significantly affected their proliferation and migration abilities. Conclusions: In conclusion, our data demonstrated that HOXA was correlated with progression and immune infiltration, and could serve as a prognostic biomarker for LGG.

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Section: Discussionmentioning

confidence: 99%

Next-generation sequencing identifies HOXA6 as a novel oncogenic gene in low grade glioma

Jiang

Wang

Guo

et al. 2022

Aging

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“…In lung cancer-related studies, it has been reported that miR-224-5p is highly expressed in NSCLC, and exocrine miR-224-5p can induce the proliferation and metastasis of NSCLC cells 30 . However, in other studies, it was found that the expression of miR-224-5p was down-regulated in lung cancer, and the over-expressed TRG-AS1 adsorbed miR-224-5p to up-regulate the expression of oncogene Smad4 in lung cancer cells to promote the proliferation and invasion of lung cancer cells 31 . Other studies have shown that circ-SHPRH regulates the expression of QKI through sponge adsorption of miR-224-5p to inhibit cadmium-induced carcinogenesis of bronchial epithelial cells 32 .…”

Section: Discussionmentioning

confidence: 88%

miR-224-5p inhibits proliferation and invasion of non-small cell lung cancer by targeting IL6ST inactivation of JAK2/STAT3 signal pathway

Tian

Xiao

et al. 2023

Preprint

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Non-small cell lung cancer (NSCLC) accounts for more than 85% of lung cancer, with high morbidity and mortality. Studies have shown that microRNA can specifically inhibit the progression of NSCLC. MiR-224-5p can regulate tumor progression in many cancers, but its function and mechanism in NSCLC aren’t clear. In this study, we found that the expression of miR-224-5p was reduced in NSCLC tissue and cells than normal lung tissue and cells. At the same time, miR-224-5p negatively regulates the proliferation and migration of NSCLC cells. Inhibition of miR-224-5p expression in A549 cells could promote cell proliferation, invasion, migration and VM formation in vitro and tumor growth and lung metastasis in vivo, while over expression of miR-224-5p in H226 cells reversed the effect. Besides, we predicted target gene and found that IL6ST is a potential target gene of miR-224-5p. The expression of miR-224-5p is negatively correlated with IL6ST and activation of JAK2/STAT3 pathway. Over expression of IL6ST reversed the effects of miR-224-5p on migration, invasion and activation of JAK2/STAT3 pathway in H226 cells. In conclusion, this study revealed that miR-224-5p can inhibit the proliferation and invasion of NSCLC by targeting inhibition of IL6ST gene transcriptional and inactivation of JAK2/STAT3 signal pathway.

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“…For instance, TRG-AS1 affects cell proliferation and invasion via miR-224-5p/SMAD4 in Lung cancer cells [57]. As a miR-4500 sponge, TRG-AS1 promotes the development of hepatocellular carcinoma [58].…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of a novel LncRNA TRG-AS1 that inhibits colorectal cancer proliferation and glycolysis

Zhang

et al. 2022

Preprint

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Abnormal glycolytic metabolism is one of the hallmarks during progression of malignant human colorectal cancer. Abundant evidence has illustrated that long non-coding RNA participated in glycolytic metabolism of various tumors. However, the lncRNA involved in the inhibition of glycolysis in CRC is largely unknown. In this study, we identified the lncRNA TRG-AS1 that inhibits CRC glycolysis in CRC by bioinformatic analysis, and its biological function was investigated in vitro and in vivo. LncRNA TRG-AS1 was identified and analyzed in The Cancer Genome Atlas database and Gene Expression Omnibus databases, and further confirmed in CRC tissues and adjacent normal tissues with qRT-PCR. TRG-AS1 overexpressing stable cell line and silence the target gene cell line were generated. Glucose assay kits and Pyruvate assay kits were utilized to analyze glycolysis. The cell proliferation ability was evaluated by CCK8, Colony formation assays and EdU. Xenograft tumor experiments were performed to assess proliferative capacity. Cell migration and invasion abilities were examined using the Transwell assay. TRG-AS1 was remarkably decreased in CRC tissues and cell lines. Higher TRG-AS1 expression is remarkably associated with more prolonged survival time. Over-expression of TRG-AS1 suppressed glycolysis, cell proliferation, invasive and migration abilities of CRC cells. On the contrary, TRG-AS1 silence attenuated CRC cells glycolysis, promoted cell proliferation, migration and invasion. Collectively, the results demonstrate that TRG-AS1 functioned as a tumor suppressor to contribute to CRC progression, is a promising prognostic factor. Our study provided a novel insight into the pathogenesis of CRC and a promising therapeutic target for CRC treatment.

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Long Non-Coding RNA TRG-AS1 Promoted Proliferation and Invasion of Lung Cancer Cells Through the miR-224-5p/SMAD4 Axis

Cited by 11 publications

References 23 publications

Next-generation sequencing identifies HOXA6 as a novel oncogenic gene in low grade glioma

Next-generation sequencing identifies HOXA6 as a novel oncogenic gene in low grade glioma

miR-224-5p inhibits proliferation and invasion of non-small cell lung cancer by targeting IL6ST inactivation of JAK2/STAT3 signal pathway

Identification and validation of a novel LncRNA TRG-AS1 that inhibits colorectal cancer proliferation and glycolysis

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