Long non-coding RNA SNHG3 promotes the progression of clear cell renal cell carcinoma via regulating BIRC5 expression

Abstract

Background: Research has shown that the progression of clear cell renal cell carcinoma (ccRCC) is modulated by long non-coding RNAs (lncRNAs). However, the roles of specific lncRNAs in the malignancy of ccRCC are still unknown.Methods: TCGA and GSE66272 datasets were used to predict differentially expressed genes (DEGs) in ccRCC. ENCORI database was employed to display BIRC5 miRNA network and potential lncRNA interactions for miRNAs. KM plotter and correlation analyses were performed to identify the overall survival (OS)-and BIRC5-related miRNAs. Quantitative real-time PCR (qRT-PCR) was used to verify the BIRC5 mRNA in the seventy paired clinical samples of ccRCC tissues. The ccRCC A498 and 786-O were individually transfected with lncRNA SNHG3 and LINC00997 and then western blotting was used to detect the BIRC5 protein expression. The Dual-luciferase reporter assay was used to examine the regulatory interaction between lncRNA SNHG3 and microRNA (miRNA/miR)-10b-5p.Results: BICR5 is associated with the progression of ccRCC. The two novel lncRNAs (LINC00997, SNHG3) were up-regulated in ccRCC tissues and positively with the BICR5 protein expression. However, Suppressing SNHG3 expression reduced BIRC5 protein expression compared with the LINC00997, most importantly, Suppressing SNHG3 expression suppressed tumor progression in vitro. In addition, SNHG3 promotes the expression of BIRC5 protein by sponging microRNA-10b-5p.Conclusions: Our findings suggest that SNHG3 plays a vital role in promoting ccRCC via the microRNA-10b-5p/BIRC5 axis and may serve as a novel therapeutic target for the treatment of patients with ccRCC.

Identification of Differentially Methylated Genes Associated with Clear Cell Renal Cell Carcinoma and Their Prognostic Values

“…When highly expressed, BIRC5 allows cancer cells to resist apoptotic checkpoints and anticancer agents [ 18 ]. Xu et al also demonstrated BICR5 is related with the development of ccRCC, which is consistent with our results [ 19 ]. BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B) has the ability to modulate the spindle assembly checkpoint (SAC) family [ 20 ].…”

Long non-coding RNAs enable precise diagnosis and prediction of early relapse after nephrectomy in patients with renal cell carcinoma

“…Although most results correspond with other works, there were some discrepancies in the direction of change in expression, namely in SNHG3 and LINC00887. While SNHG3, an autophagy-related lncRNA (Xuan et al 2021), was downregulated in our study, other works show its upregulation in RCC (Zhang et al 2019;Yang et al 2020;Xu et al 2021). Similarly, LINC00887 was downregulated in our study and up-regulated in study of Wu et al (Wu et al 2022a).…”

The LncRNA signature associated with cuproptosis as a novel biomarker of prognosis in immunotherapy and drug screening for clear cell renal cell carcinoma

“…SNHG3 promotes ccRCC cell proliferation through a TOP2A-dependent pathway ( Zhang et al, 2019 ). Baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) is another regulatory target of lncRNA SNHG3 in ccRCC ( Xu et al, 2021c ). Cuproptosis-related lncRNAs can participate in the regulation of tumorigenesis and progression by binding to DNA, miRNA, and proteins, thereby affecting tumor progression, patient prognosis, and treatment outcomes.…”