Long non-coding RNA SNHG22 facilitates hepatocellular carcinoma tumorigenesis and angiogenesis via DNA methylation of microRNA miR-16-5p

Zhang, Yinxin; Lu, Changliang; Cui, Haiwei

doi:10.1080/21655979.2021.1975969

Cited by 21 publications

(21 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DMEM containing 10% FBS was added to the lower compartment (600 µL per well). Followed by incubation at 37°C with 5% CO 2 for 24 hours, the cells that failed to migrate were gently wiped off, and the membranes were immobilized with methanol for 15 minutes and stained with 0.5% crystal violet [ 30 ]. The invaded cell number was counted under an inverted microscope (200×).…”

Section: Methodsmentioning

confidence: 99%

β-Patchoulene represses hypoxia-induced proliferation and epithelial-mesenchymal transition of liver cancer cells

Wang

Feng

et al. 2022

Bioengineered

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is a malignant tumor originating from liver epithelial cells with a high clinical mortality rate. β-Patchoulene (β-PAE) is a compound extracted from patchouli, which has analgesic, anti-inflammatory and antioxidant effects. This research aims to probe the impacts of β-PAE on hypoxia-induced HCC cell proliferation and epithelial-mesenchymal transition (EMT). Firstly, hypoxic injury models were constructed in HCC Huh-7 and MHCC97 cells, and the hypoxic injury cell models were then treated with different concentrations of β-PAE. The cell viability, proliferation, migration, invasion and apoptosis were checked by the cell counting kit-8 (CCK-8) assay, colony formation assay, Transwell assay, flow cytometry and terminal deoxyribonucleotide transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of Survivin protein, EMT markers and the NF-κB/HIF-1α pathway was gauged by Western blot (WB) or cellular immunofluorescence or reverse transcription-polymerase chain reaction (RT-PCR). The in-vivo experiment was conducted to confirm the anti-tumor role of β-PAE. As a result, β-PAE abated hypoxia-induced HCC cell growth, proliferation, migration, invasion and EMT and facilitated apoptosis in vitro and in vivo dose-dependently. Further mechanism studies displayed that β-PAE inactivated the NF-κB/HIF-1α pathway, and HIF-1α activation significantly reversed the β-PAE-mediated tumor inhibition. β-PAE repressed the proliferation and EMT of hypoxia-induced HCC cells by choking the NF-κB/HIF-1α pathway, suggesting that β-PAE was a potential drug for HCC treatment.

show abstract

Section: Methodsmentioning

confidence: 99%

β-Patchoulene represses hypoxia-induced proliferation and epithelial-mesenchymal transition of liver cancer cells

Wang

Feng

et al. 2022

Bioengineered

View full text Add to dashboard Cite

show abstract

“…RNA was extracted from nuclear/cytoplasmic fractions, and RT-qPCR was then employed to determine the expressions of LINC01133, GAPDH , and U6. GAPDH and U6 served as the cytoplasmic endogenous control, and nuclear endogenous control, respectively [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

Yin Yang 1-induced activation of LINC01133 facilitates the progression of pancreatic cancer by sponging miR-199b-5p to upregulate myelin regulatory factor expression

et al. 2022

View full text Add to dashboard Cite

Increasing evidence has reported that long non-coding RNA (lncRNA) plays a vital role in the development of pancreatic cancer (PC). However, the function and mechanism of LINC01133 in PC tumorigenesis are still unknown. Herein, we found that LINC01133 was highly expressed in PC tissues and cell lines, and LINC01133 knockdown could block the growth and metastasis of PC cells. Besides, upregulated LINC01133 in PC cells was induced by Yin Yang 1 (YY1) . Furthermore, LINC01133 directly targeted miR-199b-5p and promoted cancer malignancy by suppressing miR-199b-5p . It was also discovered that myelin regulatory factor (MYRF) was targeted by miR-199b-5p and positively correlated with LINC01133 expression in PC, and LINC01133 modulated PC progression through miR-199b-5p/MYRF pathway. In conclusion, we demonstrated that YY1 -mediated the upregulation of LINC0113 increased MYRF expression by sponging miR-199b-5p , resulting in the accelerated development of PC. These findings might offer a novel insight into the development of efficient therapeutics for PC patients.

show abstract

“…Human kidney embryonic cells (HEK293) were bought from American Type Culture Collection (ATCC, Manassas, VA). HEK293 cells were seeded in 24-well plates in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS; Gibco) and maintained in 5% CO 2 incubator at 37°C for later luciferase reporter assays [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Downregulation of microRNA-200c-3p alleviates the aggravation of venous thromboembolism by targeting serpin family C member 1

et al. 2021

View full text Add to dashboard Cite

Venous thromboembolism (VTE) is the third most prevalent cardiovascular complication. Increasing studies have demonstrated that some microRNAs (miRNAs) are aberrantly expressed in VTE and play crucial roles in mediating the development of VTE. Therefore, our study intends to explore the detailed function and molecular mechanism of miR-200c-3p in VTE progression. In our research, VTE rat models were first established via inferior vena cava (IVC) ligation and the time-dependent effects of IVC ligation on thrombus formation were discovered. The results of reverse transcription quantitative polymerase-chain reaction (RT-qPCR) and western blotting showed that serpin family C member 1 (SERPINC1) was downregulated in VTE rat models and showed an inverse correlation with thrombus load. MiRNA target prediction tools and luciferase reporter assay confirmed SERPINC1 as a target for miR-200c-3p. VTE rats were injected with miR-200c-3p inhibitor for 24 h to investigate whether miR-200c-3p knockdown influences thrombus formation in vivo . Histological examination through hematoxylin-eosin staining revealed that miR-200c-3p downregulation markedly inhibited the formation of thrombus in IVC of rats. Additionally, miR-200c-3p was upregulated while SERPINC1 was downregulated in serum and inferior vena cava of VTE rats as well as in plasma of patients with VTE. Linear regression analysis demonstrated that miR-200c-3p expression was negatively correlated to SERPINC1 expression in VTE rats and patients with VTE. Our study determines the previously unelucidated function of miR-200c-3p in VTE, which might provide a potential novel insight for the treatment of VTE.

show abstract

Long non-coding RNA SNHG22 facilitates hepatocellular carcinoma tumorigenesis and angiogenesis via DNA methylation of microRNA miR-16-5p

Cited by 21 publications

References 55 publications

β-Patchoulene represses hypoxia-induced proliferation and epithelial-mesenchymal transition of liver cancer cells

β-Patchoulene represses hypoxia-induced proliferation and epithelial-mesenchymal transition of liver cancer cells

Yin Yang 1-induced activation of LINC01133 facilitates the progression of pancreatic cancer by sponging miR-199b-5p to upregulate myelin regulatory factor expression

Downregulation of microRNA-200c-3p alleviates the aggravation of venous thromboembolism by targeting serpin family C member 1

Contact Info

Product

Resources

About