Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients

Shaker, Olfat G.; Khalil, Mahmoud A F; Elsabagh, Yumn A.; Ahmed, Azza M.; Abu-El-Azayem, Abeer K.; Gomaa, Mohamed S.; Mohammed, Asmaa

doi:10.3390/life11121382

Cited by 6 publications

(7 citation statements)

References 59 publications

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“…In this study, when compared with controls, we discovered that the expressions of miRNA 146a and miRNA 155 were significantly higher in the serum of RA patients. This finding was in agreement with numerous research that estimate the expression of both miRNAs in synovial fluid, PBMC, synovium, synovioblast and specific cells in RA patients [19][20][21][22][23][24] . Filková and colleagues 25 discovered decreased levels of miR-146a and miR-155 in the sera of individuals with early RA compared to those with chronic RA.…”

Section: Discussionsupporting

confidence: 92%

“…Our research revealed that the best cutoff of miRNA 146a in prediction of RA is ≥2.694 with area under curve 0.857, sensitivity 77.4% and specificity 80.6% (P<0.001). In support of this finding, Erfan et al 21 demonstrated that miRNA-146a can be accurate in the diagnosis of RA with an AUC of 0.836 at a cut-off value of ≥1.433 folds with a sensitivity and specificity of 82.5% and 100%, respectively. Consequently, it is able to differentiate between healthy controls and patients with early RA.…”

Section: Discussionmentioning

confidence: 76%

“…On the other hand, Erfan et al 21 showed that the expression levels of miR-146a were not correlated with DAS28, ESR, or RF. Likewise, in both synovial fluid and peripheral blood, Li et al 34 could not discover a correlation between the levels of miR-146a expression and disease activity indices.…”

Section: Discussionmentioning

confidence: 98%

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Role of miRNA146a and miRNA155 as Biomarkers in Rheumatoid Arthritis Disease Activity

Esmaeel,

Kamal,

Hassan

et al. 2024

Egyptian Journal of Medical Microbiology

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Background: Rheumatoid arthritis (RA) represents a worldwide health concern as it leads to joint deformities or even permanent disability. As epigenetic directors of biological signalling mechanisms, the erratic expression of various micro-RNAs (miRNAs) is strongly associated with RA. Objectives: The purpose of the current research was to assess the significance of miRNA-146a and miRNA-155 as predictors of the development of RA. We also assessed their promising application as biomarkers for RA disease activity. Methodology: This case-control study included 62 subjects (31 RA patients and 31 healthy controls). Participants sera were used for laboratory tests [anti-cyclic citrullinated peptide (anti-CCP), rheumatoid factor (RF), erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP)] and for the measurement of miRNA-146a, and miRNA-155 gene expression. Results: In RA patients, levels of miRNA-146a and miRNA-155 were significantly higher than in controls (P<0.001). The receiver operating characteristic (ROC) curve analysis showed that miRNA-146a could predict RA with area under curve 0.857, sensitivity 77.4% and specificity 80.6% (P<0.001) at 95% confidence interval (CI) (0.765 -0.95). MiRNA-155 could predict RA with area under curve 0.829, sensitivity 77.4% and specificity 67.7% (P<0.001) at 95% . Active RA patients expressed more miRNA-146a and miRNA-155 than the inactive group. There was a significant positive correlation (P<0.05) among miRNAs-146a and155 expression levels and DAS-28 score in RA patients. MiRNA -146a was found to increase the risk of active RA significantly independently by 2.023 folds. The ROC curve showed that miRNA -146a is useful for prediction of RA disease activity with area under curve 0.905, sensitivity 91.3% and specificity 87.5% (P<0.001) at 95% CI (0.791 -1). Conclusion: MiRNA-146a and miRNA-155 expressions were remarkable in RA patients and could be recognized as potential biomarkers for early RA diagnosis. However, miRNA-146a expression is an independent risk for active RA and could be a valuable biomarker to predict RA disease activity.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 76%

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Role of miRNA146a and miRNA155 as Biomarkers in Rheumatoid Arthritis Disease Activity

Esmaeel,

Kamal,

Hassan

et al. 2024

Egyptian Journal of Medical Microbiology

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“…The disorder of microRNA profile acts as a biomarker event for RA progression [ 6 ]. Moreover, accumulating reports have indicated that miRNAs directly regulate RA development via influencing the proliferation, inflammatory level, and cellular molecular signal transduction of RA-FLSs [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-129-5p Inactivates NF-κB Pathway to Block Rheumatoid Arthritis Development via Targeting BRD4

Chen

2022

Journal of Healthcare Engineering

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Object. Rheumatoid arthritis (RA) is an osteoarticular disease which seriously affects the motor abilities of the patients. MicroRNA disorder has been confirmed as a biomarker event in RA development, and several studies have identified that miR-129-5p is related with the progression of multiple bone diseases. The study attempted to investigate the regulation mechanism of miR-129-5p in RA development. Methods. The abundance of miR-129-5p was detected in the samples including normal tissues and RA tissues and cell lines including human fibroblast-like synoviocytes (hFLSs) and human rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs). The CCK-8 assay, flow cytometry, Transwell, and ELISA were used to observe the effects of miR-129-5p on the phenotype of RA-FLSs. Moreover, the potential targets of miR-129-5p were identified with TargetScan and dual-luciferase reporter gene assay. Besides, the abundances of the proteins were analyzed with western blot. Results. Decreased miR-129-5p was observed in RA tissues and cells. Increased miR-129-5p obviously blocked the proliferation, inflammatory stress, and migration and remarkably promoted cellular apoptosis. Moreover, BRD4 was confirmed as targets of miR-129-5p, and BRD4 upregulation could partly rescue the inhibition of miR-129-5p on aggressive behaviors of RA-FLSs. Besides, the finding of this study also proved that upregulated miR-129-5p could impede the NF-κB pathway via targeting BRD4. Conclusion. This study suggests that miR-129-5p suppresses the activation of NF-κB pathway to block the progression of RA via targeting BRD4.

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“…LINK-A regulates RA FLS invasion and inflammation through HIF-1α and/or miR-1262 pathways, which might be a promising therapeutic target for RA ( 152 ). lncRNA growth arrest-specific transcript 5 (GAS5) is significantly downregulated in synovial tissues, serum, and PBMCs of RA patients compared with HCs and negatively correlated with IL6, IL-17, CRP, ESR, DAS28, and anti-CCP, suggesting that it could be used as a potential biomarker for RA diagnosis ( 153 – 156 ). Interestingly, lncRNAs GAS5 (3.31-fold), RNA component of mitochondrial RNA-processing endoribonuclease (RMRP) (2.43-fold), and TNF-α and heterogeneous nuclear ribonucleoprotein L (THRIL) (2.14-fold) were significantly upregulated in the circulating T cells of RA patients compared with controls, and the ROC curve analysis of the three indicated their value in discriminating RA patients from controls ( 157 ).…”

Section: Candidate Rnas As Biomarkers For Ramentioning

confidence: 99%

Biomarkers (mRNAs and non-coding RNAs) for the diagnosis and prognosis of rheumatoid arthritis

Jiang

Zhong

et al. 2023

Front. Immunol.

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In recent years, diagnostic and therapeutic approaches for rheumatoid arthritis (RA) have continued to improve. However, in the advanced stages of the disease, patients are unable to achieve long-term clinical remission and often suffer from systemic multi-organ damage and severe complications. Patients with RA usually have no overt clinical manifestations in the early stages, and by the time a definitive diagnosis is made, the disease is already at an advanced stage. RA is diagnosed clinically and with laboratory tests, including the blood markers C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) and the autoantibodies rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPA). However, the presence of RF and ACPA autoantibodies is associated with aggravated disease, joint damage, and increased mortality, and these autoantibodies have low specificity and sensitivity. The etiology of RA is unknown, with the pathogenesis involving multiple factors and clinical heterogeneity. The early diagnosis, subtype classification, and prognosis of RA remain challenging, and studies to develop minimally invasive or non-invasive biomarkers in the form of biofluid biopsies are becoming more common. Non-coding RNA (ncRNA) molecules are composed of long non-coding RNAs, small nucleolar RNAs, microRNAs, and circular RNAs, which play an essential role in disease onset and progression and can be used in the early diagnosis and prognosis of RA. In this review of the diagnostic and prognostic approaches to RA disease, we provide an overview of the current knowledge on the subject, focusing on recent advances in mRNA–ncRNA as diagnostic and prognostic biomarkers from the biofluid to the tissue level.

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Long Non-Coding RNA PVT1 and Its Target miRNA-146a as Potential Prognostic Biomarkers in Rheumatoid Arthritis Patients

Cited by 6 publications

References 59 publications

Role of miRNA146a and miRNA155 as Biomarkers in Rheumatoid Arthritis Disease Activity

Role of miRNA146a and miRNA155 as Biomarkers in Rheumatoid Arthritis Disease Activity

MiR-129-5p Inactivates NF-κB Pathway to Block Rheumatoid Arthritis Development via Targeting BRD4

Biomarkers (mRNAs and non-coding RNAs) for the diagnosis and prognosis of rheumatoid arthritis

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