Long non-coding RNA plasmacytoma variant translocation 1 linked to hypoxia-induced cardiomyocyte injury of H9c2 cells by targeting miR-135a-5p/forkhead box O1 axis

Abstract

Background Myocardial infarction occurs due to insufficient (ischemia) blood supply to heart for long time; plasmacytoma variant translocation 1 (PVT1) is a long non-coding RNAs (lncRNAs) involved in the pathogenesis of various diseases, including heart disease; However, few studies have explored its role. The present study evaluated the effects of lncRNA PVT1 on hypoxic rat H9c2 cells. Methods Hypoxic injury was examined by measuring cell viability and apoptosis by using cell counting kit-8 activity and flow cytometry assays. Gene expressions after hypoxia were estimated by quantitative real time polymerase chain reaction and the signaling pathway were explored by Western blot analysis. RNA immunoprecipitation and luciferase reporter assays were applied to examine the interactions among genes. Data were analyzed using t -test with one-way or two-way analysis of variance. Results The lncRNA PVT1 is up-regulated in hypoxia-stressed H9c2 cells and knockdown of PVT1 mitigates hypoxia-induced injury in H9c2 cells. PVT1 acts as a sponge for miR-135a-5p and knockdown of PVT1 attenuated the increased hypoxia-induced injury by up-regulating miR-135a-5p. Forkhead box O1 ( FOXO1 ) was identified as a target of miR-135a-5p, and the expression was negatively regulated by miR-135a-5p. The exploration of the underlying mechanism demonstrated that knockdown of FOXO1 reversed PVT1/miR-135a-5p mediated hypoxia-induced injury in H9c2 cells. Conclusions PVT1 plays a crucial role in hypoxia-injured H9c2 cells through sponging miR-135a-5p and then positively regulating FOXO1 .

Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction

“… 37 Xu et al demonstrated that miR-135a-5p negatively regulated hypoxia-induced cardiomyocyte injury in H9c2 cells. 38 CENPB, a cell cycle-related gene, was reported to be differentially regulated in the heart tissue in serum-response factor null embryonic stem cells. 39 BCL9L is a nuclear co-activator in the Wnt/β-catenin pathway, which was shown to be associated with various cancers.…”

Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis

“…CircRNA is one of the ncRNA, which is thought to encode almost no proteins [1] . Although ncRNA plays a huge role in the process of life, participating in biological growth, [15] differentiation, [16] metabolism, [17] disease occurrence, [18–20] and other processes, its specific mechanism is still an unsolved mystery. Studies have shown that circRNA can participate in the occurrence of many autoimmune diseases and can regulate the course of diseases by regulating the body's immune response [21] and immune tolerance [22] .…”