Identification of ceRNA (lncRNA-miRNA-mRNA) Regulatory Network in Myocardial Fibrosis After Acute Myocardial Infarction

Abstract

Purpose Myocardial fibrosis (MF) after acute myocardial infarction (AMI) ultimately results in heart failure, which is a serious threat to human life. This study aimed to excavate critical biomarkers associated with MF after AMI. Materials and Methods RNA-sequencing was performed to obtain differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs) in AMI and MF after AMI. Results Abundant DEmRNAs, DEmiRNAs and DElncRNAs were identified in AMI and MF after AMI. The ceRNA network, which contained 9 lncRNA-miRNA pairs and 9 miRNA-mRNA pairs, was acquired. In AMI, all candidate markers generally exhibited the same pattern as that in our RNA-seq results; while in MF after AMI, except for CENPB, JAK2 and hsa-miR-197-3p, the expression of the others in the qRT-PCR results exhibited the same pattern as that in our RNA-seq results. Conclusion We speculated that LINC00664/hsa-miR-197-3p/JAK2 and GAS6-AS1/SNHG22/hsa-miR-135a-5p/CENPB/BCL9L interaction pairs may serve as potential biomarkers in MF after AMI.

The Importance of Decreased Expression Levels of BAT5 and IL21R-AS1 in Circulating Leukocytes of Patients with Acute Myocardial Infarction

“…Of this IL21R-AS1 and LEF1-AS1 were validated by qRT-PCR. In this study, it was shown that IL21R-AS1 and LEF1-AS1 were down-regulated in qRT-PCR with the same pattern as RNA sequencing [35]. This study also supports our study, reminding us to focus on the roles of IL21R-AS1 in AMI.…”

Knockdown of long noncoding RNA MIAT attenuates hypoxia‐induced cardiomyocyte injury by regulating the miR‐488‐3p/Wnt/β‐catenin pathway

“…Currently, some studies have indicated that lncRNAs could play an important regulatory role in the development of AMI through their ceRNA activity (H. Liu et al, 2019; S. Wang, Liu, et al, 2021). For instance, silencing of PVT1 might suppress hypoxia/reoxygenation‐induced AC16 cardiomyocyte apoptosis by acting as a ceRNA for miR‐186 (Ouyang et al, 2020).…”

“…To further assess whether the effect of MIAT on cardiomyocyte injury was mediated by Wnt5a, cells were cotransfected with Wnt5a Currently, some studies have indicated that lncRNAs could play an important regulatory role in the development of AMI through their ceRNA activity (H. Liu et al, 2019;S. Wang, Liu, et al, 2021).…”

“…In addition, numerous studies have suggested that lncRNAs are involved in gene regulation at the post‐transcriptional level and repress miRNA activity via acting as competing endogenous RNAs (ceRNAs) (Paraskevopoulou & Hatzigeorgiou, 2016). Lately, it has become evident the importance of lncRNA‐mediated ceRNA activity in the pathogenesis of AMI (S. Wang, Liu, et al, 2021). For example, Pan et al (2021) unveiled that silencing of MIAT reduced LPS‐induced injury in chondrocytes via regulating miR‐488‐3p.…”

Long Noncoding RNA SNHG4 Attenuates the Injury of Myocardial Infarction via Regulating miR-148b-3p/DUSP1 Axis

“…The translation or degradation of target mRNAs is the main regulatory outcomes of lncRNAs/miRNAs in cardiovascular diseases [ 19 ]. There are many lncRNA/miRNA/mRNA regulatory axes that have been determined in MI, such as LINC00664/miR-197-3p/JAK2 [ 21 ], MALAT1/miR-30a/BECN1 [ 22 ], GAS5/miR-525-5p/CALM2 [ 23 ], and PCFL/miR-378/GRB [ 24 ]. Dual specificity phosphatase 1 (DUSP1) is an antiapoptotic phosphatase that is highly expressed in normal human organ tissues, such as liver, heart, and lung [ 23 ].…”