Long non‑coding RNA NR_002794 is upregulated in pre‑eclampsia and regulates the proliferation, apoptosis and invasion of trophoblast cells

Abstract

Pre-eclampsia is a common complication during pregnancy, characterized by hypertension and proteinuria. The pathogenesis of pre-eclampsia is not fully understood. Studies on the maternal spiral artery have led scientists to consider that the ineffective infiltration of placental trophoblast cells may be a primary cause of pre-eclampsia. The present study aimed to investigate the differences in the profiles of long non-coding RNAs (lncRNAs) between the placentas of patients with pre-eclampsia and those of healthy pregnant women. The involvement of the differentially expressed lncRNAs in the biological activity of trophoblast cells was also assessed. A total of 26 differentially expressed lncRNAs were identified between the pre-eclampsia and healthy groups. Upregulation of NR_002794 was found in tissues from patients with pre-eclampsia. In SWAN71 trophoblast cells, NR_002794 had suppressive effects on proliferation and migration, and resulted in an increased rate of apoptosis. Furthermore, lncRNA NR_002794 had no effect on the phagocytosis of trophoblast cells. The present study suggested that abnormal levels of NR_002794 may lead to atypical conditions in trophoblast cells, which may be associated with the failure of maternal spiral artery remodeling during pregnancy and, consequently, with the development of pre-eclampsia.

Roles of noncoding RNAs in preeclampsia

“… [ 171 ] NR_002794 placenta upregulated / NR_002794 has suppressive effects on proliferation and migration, and results in an increased rate of apoptosis. [ 172 ] MVIH placenta downregulated Jun-B The silencing of MVIH expression inhibits cell growth, migration, invasion, and angiogenesis in various trophoblast cell lines by modulating Jun-B protein expression. [ 173 ] GASAL1 placenta downregulated SRSF1/mTOR/EBP1/Bcl-2/caspase-3 lncRNA GASAL1 interacts with SRSF1 to regulate the proliferative, invasive, and apoptotic abilities of trophoblast cells via the mTOR signaling pathway.…”

miRNAs in decidual NK cells: regulators worthy of attention during pregnancy

“…Deficiency of invaded trophoblastic cells is the one of main causes of dysfunctional placentas. Inadequate motility of trophoblastic cells leads to abnormal remodeling of spiral arteries and disrupts material exchange between fetus and mother, thus causing a series of pregnancy-related disorders [31,32]. Earlier study has revealed that trophoblast migration and invasion are correlated with the enhanced ability of dNK cells to produce IL-8 and IP-10, which bind to C-X-C motif chemokine receptor 1(CXCR1) and CXCR3 expressed by invasive EVT, respectively [33].…”

lncRNA HIF1A‑AS2: A potential oncogene in human cancers (Review)

“…Although PE has been extensively studied ( 11 ), the underlying pathogenesis of PE remains elusive. However, it is hypothesized that inadequate trophoblastic invasion may cause PE ( 12 , 13 ). Wu et al ( 14 ) reported that HIF1A-AS2 expression was significantly downregulated in the tissues of 52 patients with PE compared with the adjacent normal samples.…”