Long non-coding RNA Linc00675 suppresses cell proliferation and metastasis in colorectal cancer via acting on miR-942 and Wnt/β-catenin signaling

Shan, Zhiming; An, Na; Qin, Jielin; Yang, Junmei; Sun, Harold; Yang, Wei

doi:10.1016/j.biopha.2018.02.123

Cited by 58 publications

(64 citation statements)

References 23 publications

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“…On the other hand, there are 11 down‐regulated lincRNAs in WGCNA modules which play a potential role of tumor suppressor (including LINC00675 , RP11‐473 M20.11 , RP11‐661A12.4 , LINC01857 , AP001056.1 , TSPOAP1‐AS1 , RP11‐356O9.2 , WI2‐1959D15.1 , RP11‐1008C21.1 , RP4‐666F24.3 and RP11‐101C21.1 ). For example, LINC00675 (ENSG00000263429) in module M4 was reported to suppress cell proliferation and metastasis in colorectal cancer and suppress gastric cancer progression . Down‐regulation of RP11‐473 M20.11 (ENSG00000263011) in module M5 was verified by real‐time quantitative RT‐PCR in lung adenocarcinoma .…”

Section: Resultsmentioning

confidence: 99%

Systematic identification of lincRNA‐based prognostic biomarkers by integrating lincRNA expression and copy number variation in lung adenocarcinoma

Wang

Zhao

et al. 2018

Intl Journal of Cancer

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Copy number alterations (CNAs) of lincRNAs act as one of important mechanisms in disrupting lincRNA expression which may play critical roles during tumorigenesis in lung adenocarcinoma (LUAD). The copy number alterations of lincRNAs can mark the spectrum of cancer progression and may serve as biomarkers for prognosis in LUAD, however it is rarely studied. We analyzed RNASeq data for 488 LUAD patients from TCGA portal and 58 healthy subjects to identify prognostic lincRNAs predictive of patient survival. Computational analysis entailing integration of expression and copy number alteration data revealed five prognostic lincRNAs: RBPMS-AS1, TDRKH-AS1, LINC00578, RP11-470M17.2 and LINC00941. The copy number alterations in the LINC00578 and RP11-470M17.2 genes were positively associated with the longer overall survival of LUAD patients. The CNA in LINC00941 was negatively associated with the longer overall survival. Copy number amplification significantly correlated with increased expression of TDRKH-AS1, which regulates telomere organization and EZH2-mediated epigenetic silencing of CDKN1A, CDKN1B and IL24. Decreased survival of LUAD patients was associated with high LINC00941 expression. The LINC00941 regulates the PI3K-AKT signaling pathway, focal adhesion by influencing potential targets, such as KRAS proto-oncogene GTPase and VEGFC. These lincRNA-based prognostic biomarkers may destroy important cancer-related biological processes contributing to LUAD prognosis. In summary, we demonstrate the prognostic potential of four differentially expressed lincRNAs with copy number alterations (RBPMS-AS1, TDRKH-AS1, LINC00578 and RP11-470M17.2) that are positively associated with longer overall survival of LUAD patients. One differentially expressed lincRNA LINC00941 with copy number alterations was negatively associated with longer overall survival of LUAD patients. This article is protected by copyright. All rights reserved.

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Section: Resultsmentioning

confidence: 99%

Systematic identification of lincRNA‐based prognostic biomarkers by integrating lincRNA expression and copy number variation in lung adenocarcinoma

Wang

Zhao

et al. 2018

Intl Journal of Cancer

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“…7 Downregulation of LINC00675 was identified in both colon cancer tissues and cell lines, and ectopic expression of LINC00675 inhibited proliferation, invasion and migration of colon cell lines by sponging miR-942. 8 Lymphoid enhancer-binding factor 1 (LEF1) antisense RNA 1 (LEF1-AS1) is a highly conserved and newly discovered long non-coding RNA encode in the plus strand of LEF1 at chromosome 4q25. Many studies have demonstrated that LEF1-AS1 is enrolled in the tumorigenesis of a variety of cancer, such as glioblastoma, 9 oral squamous cell carcinoma, 10 non-small-cell lung cancer 11 and prostate cancer.…”

Section: Introductionmentioning

confidence: 99%

<p>Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis</p>

Sun¹,

Liu²,

Zhang³

et al. 2020

OTT

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Introduction: Aberrant expression of long non-coding RNAs (lncRNAs) has been implicated in the tumorigenesis and progression of colon cancer. Lymphoid enhancer-binding factor 1 antisense RNA 1 (LEF1-AS1), a highly conserved and newly discovered long noncoding RNA, has been reported to be upregulated and correlated with poor prognosis in colon cancer, but the exact role of it remains uncertain. Materials and Methods: In our study, the biological functions of LEF1-AS1 in colon cancer were analyzed by cell viability assay, colony formation assay, scratch wound healing assay, transwell cell invasion assay, soft agar assay, luciferase reporter assay, pull down assay, tumor xenograft model and Western blot. Results: We found that LEF1-AS1 was upregulated in colon cancer patients and correlated with poor overall survival and recurrent-free survival. Besides, enforced expression of LEF1-AS1 in HT29 and T84 cells promoted migration, invasion, anchorage-independent growth, tumor xenograft formation and lung metastasis, while knockdown of LEF1-AS1 in COLO320 cells suppressed cell migration, invasion, anchorage-independent growth and tumor xenograft formation. In addition, LEF1-AS1 was directly interacted and inversely correlated with miR-30-5p in colon cancer, and SOX9 was a downstream target for miR-30-5p. LEF1-AS1 overexpression increased the expression level of SOX9, and restoration of SOX9 attenuated the effects caused by LEF1-AS1 knockdown in cell migration, invasion, anchorage-independent growth and tumor xenograft formation. Conclusion: Our results indicated that LEF1-AS1 promoted migration, invasion and metastasis of colon cancer cells partially through miR-30-5p/SOX9 axis. The oncogenic LEF1-AS1 could be a potential prognostic biomarker for colon cancer.

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“…15 Here, we found that circEPSTI1 could bind to miR-942 and acted as a miRNA sponge. 18 Here, we found that miR-942 was down-regulated in ovarian cancer. Forced expression of miR-942 significantly changes the drug sensitive cells to resistant in hepatocellular carcinoma and gastric cancer.…”

Section: Discussionmentioning

confidence: 65%

“…17 In colorectal cancer, long non-coding RNA linc00675 suppresses cell proliferation and metastasis via regulating miR-942. 18 Here, we found that miR-942 was down-regulated in ovarian cancer.…”

Section: Discussionmentioning

confidence: 65%

circEPSTI1 regulates ovarian cancer progression via decoying miR‐942

Xie

Wang

et al. 2019

J Cellular Molecular Medi

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Increasing studies show that circular RNAs (circRNAs) play vital roles in tumour progression. But, how circRNAs function in ovarian cancer is mostly unclear. Here, we detected the expression of circEPSTI1 in ovarian cancer and explored the function of circEPSTI1 in ovarian cancer via a series of experiments. Then, we performed luciferase assay and RNA immunoprecipitation (RIP) assay to explore the competing endogenous RNA (ceRNA) function of circEPSTI1 in ovarian cancer. qRT‐PCR verified that circEPSTI1 was overexpressed in ovarian cancer. Inhibition of circEPSTI1 suppressed ovarian cancer cell proliferation, invasion but promoted cell apoptosis. Luciferase assays and RIP assay showed that circEPSTI1 and EPSTI1 (epithelial stromal interaction 1) could directly bind to miR‐942. And circEPSTI1 could regulate EPSTI1 expression via sponging miR‐942. In summary, circEPSTI1 regulated EPSTI1 expression and ovarian cancer progression by sponging miR‐942. circEPSTI1 could be used as a biomarker and therapeutic target in ovarian cancer.

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Long non-coding RNA Linc00675 suppresses cell proliferation and metastasis in colorectal cancer via acting on miR-942 and Wnt/β-catenin signaling

Cited by 58 publications

References 23 publications

Systematic identification of lincRNA‐based prognostic biomarkers by integrating lincRNA expression and copy number variation in lung adenocarcinoma

Systematic identification of lincRNA‐based prognostic biomarkers by integrating lincRNA expression and copy number variation in lung adenocarcinoma

<p>Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis</p>

circEPSTI1 regulates ovarian cancer progression via decoying miR‐942

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