&lt;p&gt;Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis&lt;/p&gt;

Sun, Ting; Liu, Zhexian; Zhang, Rui; Ma, Shenglin; Lin, Tao; Li, Yanxi; Yang, Shun Tai; Zhang, Wanchuan; Wang, Yongpeng

doi:10.2147/ott.s232839

Cited by 25 publications

(20 citation statements)

References 46 publications

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“…To further explore the underlying functions of lncRNAs in ACS, co-expression of lncRNAs with their associated coding genes was performed based on the degree of correlation. Among the top 10 elevated and decreased lncRNAs according to the degree, NR_029374 with 12 degrees was upregulated, consistent with the results obtained by Sun et.al [34] , who reported that NR_029374 was upregulated in CC and correlated with poor overall survival. NR_029374 promoted the migration, invasion, and metastasis of CC through the miR-30-5p/SOX9 axis and this nding was supported by previous results [35] .…”

Section: Discussionsupporting

confidence: 91%

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

et al. 2020

Preprint

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Abstract Background DNA mismatch repair proficient colon cancer (pMMR CC) is the most common subtype of sporadic CC. However, the role of long non-coding RNAs (lncRNAs) in pMMR CC carcinogenesis has not been fully elucidated. Methods In the present study, we conducted transcriptomic analysis of lncRNAs-mRNAs in five low-grade intraepithelial neoplasia (LGIN), five high-grade intraepithelial neoplasia (HGIN), four pMMR CC, and five normal control (NC) tissues. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment pathway, and Co-Expression Network analyses were performed to elucidate the functions of lncRNAs and mRNAs as well as their interactions. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate five dysregulated lncRNAs in a large set of colon tissues. Receiver-operating characteristic (ROC) curves were employed to evaluate the performance of the candidate lncRNAs. Results A set of 5783 differentially expressed lncRNAs and 4483 differentially expressed mRNAs were detected among the LGIN, HGIN, pMMR CC, and NC samples. These differentially expressed lncRNAs and mRNAs were assigned to 275 significant GO terms and 179 significant KEGG enriched pathways. qRT-PCR confirmed that the expression of five selected lncRNAs (ENST00000521815, ENST00000603052, ENST00000609220, NR_026543, and ENST00000545920) were consistent with the microarray data. ROC analysis showed that four lncRNAs (ENST00000521815, ENST00000603052, ENST00000609220, and NR_026543) had larger area under the ROC curve (AUC) values than serum carcinoembryonic antigens, thereby distinguishing NC from pMMR CC. Conclusions Several lncRNAs play various roles in the adenoma–carcinoma sequence and may serve as potential biomarkers for the early diagnosis of pMMR CC.

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Section: Discussionsupporting

confidence: 91%

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

et al. 2020

Preprint

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“…Biotinylated Bio‐NC, Bio‐miR‐362‐5p‐wt, and Bio‐miR‐362‐5p‐mut were selected and the assay was performed as previously described 19 . GAS5 expression was detected by RT‐qPCR.…”

Section: Methodsmentioning

confidence: 99%

LncRNA GAS5 sponges miR‐362‐5p to promote sensitivity of thyroid cancer cells to ¹³¹I by upregulating SMG1

Lin

et al. 2020

IUBMB Life

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This study aims to investigate the role of lncRNA growth arrest-specific transcript 5 (GAS5)/miR-362-5p/suppressor of morphogenesis in the genitalia 1 (SMG1) axis in 131 I-resistance in thyroid cancer (TC). GAS5, miR-362-5p, and SMG1 expression in TC tissues was assessed and the 131 I-resistant TC cells were established, which were treated with altered GAS5, miR-362-5p, and SMG1. The proliferation and apoptosis of 131 I-resistant TC cells were detected, and the expression of Akt/mTOR signaling pathway-related proteins was assessed. Binding relations between GAS5 and miR-362-5p, and miR-362-5p and SMG1 were confirmed. The role of GAS5 in 131 I-resistant TC cell growth in vivo was observed. GAS5 was downregulated and miR-362-5p was upregulated in TC tissues and 131 I-resistant cells. The 131 I-resistant TC cells had enhanced proliferation and repressed apoptosis, and the Akt/mTOR signaling pathway was activated. Overexpressed GAS5 strengthened 131 I sensitivity and suppressed TC cell growth, while upregulated miR-362-5p had an opposite effect. MiR-362-5p upregulation reversed the effect of GAS5, and SMG1 overexpression eliminated the impact of miR-362-5p upregulation on 131 I-resistant TC cells. GAS5 competitively binds to miR-362-5p and SMG1 is targeted by miR-362-5p. GAS5 sponges miR-362-5p to promote sensitivity of TC cells to 131 I by upregulating SMG1 and inactivating Akt/mTOR signaling pathway.

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“…LncRNA-lymphoid enhancer-binding factor 1 antisense RNA 1 (lncRNA-LEF1-AS1) is highly expressed in colorectal cancer and positively correlated with the dismal prognosis of patients [ 247 ]. In this study, it was further observed that lncRNA-LEF1-AS1 heightens the capability of colorectal cells to grow under anchorage-independent conditions and metastasize to lungs through repressing the activity of miR-30-5p to target the sex-determining region Y box 9 (SOX9) [ 247 ], which is a pro-survival, -stemness, and -metastasis factor [ 261 , 262 , 263 ]. In line with this, SOX9 was described to support anoikis resistance by promoting the stemness property of cancer cells [ 264 ] ( Figure 3 and Table 3 ).…”

Section: Long Noncoding Rnas Anoikis and Anchorage-independent Gmentioning

confidence: 99%

The Role of Noncoding RNAs in the Regulation of Anoikis and Anchorage-Independent Growth in Cancer

Lee

Son

Moeng

et al. 2021

IJMS

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Cancer is a global health concern, and the prognosis of patients with cancer is associated with metastasis. Multistep processes are involved in cancer metastasis. Accumulating evidence has shown that cancer cells acquire the capacity of anoikis resistance and anchorage-independent cell growth, which are critical prerequisite features of metastatic cancer cells. Multiple cellular factors and events, such as apoptosis, survival factors, cell cycle, EMT, stemness, autophagy, and integrins influence the anoikis resistance and anchorage-independent cell growth in cancer. Noncoding RNAs (ncRNAs), such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs), are dysregulated in cancer. They regulate cellular signaling pathways and events, eventually contributing to cancer aggressiveness. This review presents the role of miRNAs and lncRNAs in modulating anoikis resistance and anchorage-independent cell growth. We also discuss the feasibility of ncRNA-based therapy and the natural features of ncRNAs that need to be contemplated for more beneficial therapeutic strategies against cancer.

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<p>Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis</p>

Cited by 25 publications

References 46 publications

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

LncRNA GAS5 sponges miR‐362‐5p to promote sensitivity of thyroid cancer cells to ¹³¹I by upregulating SMG1

The Role of Noncoding RNAs in the Regulation of Anoikis and Anchorage-Independent Growth in Cancer

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<p>Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis</p>

Cited by 25 publications

References 46 publications

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

Comprehensive Analysis of Differentially Expressed Long Non-Coding RNA-mRNA in The Adenoma–Carcinoma Sequence of DNA Mismatch Repair Proficient Colon Cancer

LncRNA GAS5 sponges miR‐362‐5p to promote sensitivity of thyroid cancer cells to 131I by upregulating SMG1

The Role of Noncoding RNAs in the Regulation of Anoikis and Anchorage-Independent Growth in Cancer

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LncRNA GAS5 sponges miR‐362‐5p to promote sensitivity of thyroid cancer cells to ¹³¹I by upregulating SMG1