Long non-coding RNA AGER-1 inhibits colorectal cancer progression through sponging miR-182

Abstract

Objective: Abundant evidence has illustrated that long non-coding RNA (lncRNA) plays a vital role in the regulation of tumor development and progression. Ectopic expression of a novel lncRNA, termed lnc-AGER-1, has been discovered in cancers, and this lncRNA was reported to exert an anti-tumor effect. However, its biological mechanism remains unelucidated in colorectal cancer. Methods: A total of 159 paired colorectal cancer specimens and adjacent tissues was applied to detect the expression of lnc-AGER-1 by the quantitative Real-time PCR (qRT-PCR), and a series of functional assays was executed to uncover the role of this lncRNA on colorectal cancer. Results: We found that the expression of lnc-AGER-1 in the tumor tissues was significantly down-regulated, while compared with adjacent normal tissues (0.0115 ± 0.0718 vs. 0.0347 ± 0.157; P < 0.0001). Also, lnc-AGER-1 was observably associated with clinical T status (r = −0.184, P = 0.024). Patients with advanced T status exerted a significantly lower level of lnc-AGER-1 than those with early T status (20.0% vs. 40.7%, P = 0.021). Over-expression of lnc-AGER-1 inhibited cell proliferation and migration efficiency, and induced cell cycle arrest at the G0/G1 phase, and promoted cell apoptosis. Further research proved that lnc-AGER-1 altered the expression of its neighbor gene, AGER, through acting as a competing endogenous RNA for miR-182 in colorectal cancer. Conclusion: lnc-AGER-1 has a suppressive role in colorectal cancer development via modulating AGER, which may serve as a target for colorectal cancer diagnosis and treatment.

Lnc‐CPLC promotes the progression of colorectal cancer via regulating ZBTB34 by competitively binding miR‐4319

“…Studies on the molecular mechanism of CRC have always attracted the attention of scientists. Previous studies have found that the occurrence and development of CRC may be closely related to the molecular regulation of noncoding RNA (Gu et al, 2020; Lin et al, 2020; W. Peng et al, 2020; Zhang et al, 2020). Noncoding RNAs are not involved in the expression of proteins in cells, but they can affect the regulation of proteins at multiple levels and are widely involved in molecular signaling pathways in cells.…”

“…Studies on the molecular mechanism of CRC have always attracted the attention of scientists. Previous studies have found that the occurrence and development of CRC may be closely related to the molecular regulation of noncoding RNA (Gu et al, 2020;Lin et al, 2020;W. Peng et al, 2020;Zhang et al, 2020).…”

lncRNA HCG11 Promotes Colorectal Cancer Cell Malignant Behaviors via Sponging miR-26b-5p

“…Accumulating researches have unmasked the wide participation of lncRNAs in the regulation of CRC cell biological behaviors and tumor progression [ 18 , 28 ]. Specifically, AGER-1 exerts inhibitory influences on CRC progression via sponging miR-182 [ 29 ] while SNHG10 contributes to exacerbated malignant behaviors of CRC cells via targeting miR-3690 [ 30 ]. In this study, we aimed to fathom out the functional role of HCG11 in the regulation of CRC cell malignant behaviors.…”

Long Noncoding RNA DSCAM-AS1 Facilitates Colorectal Cancer Cell Proliferation and Migration via miR-137/Notch1 Axis

“…The ceRNA hypothesis was proposed to describe the function of many lncRNAs as ceRNA to protect the genuine targets of miRNAs from silencing or translational suppression 26 . LncRNA-miRNA-mRNA axis has been reported to play important roles in CRC 27 - 29 . Although the expression and function of DSCAM-AS1 have been reported in previous studies in several tumors, the potential mechanisms involved have not been clarified.…”