Lnc‐CPLC promotes the progression of colorectal cancer via regulating ZBTB34 by competitively binding miR‐4319

Abstract

Long noncoding RNAs (lncRNAs) have been considered as regulatory molecules that play crucial roles in diverse biological processes, including the regulation of tumor progression. However, in colorectal cancer (CRC), due to the complex regulatory relationships involved in lncRNAs, the details of the specific mechanism still need to be elucidated. To discover the key regulatory role of lncRNA in CRC, we used bioinformatics analysis methods for preliminary screening. Through the combination of in vitro and in vivo verification, we further comprehensively analyzed the specific regulation of the key gene and the related key lncRNA in CRC. We found that ZBTB34 and lnc-CPLC (CRC progression-associated lncRNA) had a strong correlation, which plays a key role in the regulation of CRC. Furthermore, by exerting the "sponge" function, lnc-CPLC could bind to miR4319 and release its binding to the 3'UTR of ZBTB34 mRNA. Our results reveal the mechanism of the lnc-CPLC/miR-4319/ZBTB34 signal axis in CRC and provide evidence for elucidating the complex molecular mechanisms in tumors.

Long non-coding RNA SLC25A21-AS1 inhibits the development of epithelial ovarian cancer by specifically inducing PTBP3 degradation

“…Typically, lncRNAs act via target molecules [ 39 ], such as lncPOP1-1, that promotes cisplatin resistance in squamous cell carcinoma, which occurs in the neck, by interacting with MCM5 [ 40 ]. In addition, lncCPLC promotes colorectal resistance by regulating cancer progression via interaction with ZBTB [ 41 ]. At the same time, some lncRNAs in OC have also been reported to control OC resistance [ 42 ] or autophagy.…”

Long non-coding RNA SLC25A21-AS1 inhibits the development of epithelial ovarian cancer by specifically inducing PTBP3 degradation

“…Typically, lncRNAs act via target molecules [39] , such as lncPOP1-1, that promotes cisplatin resistance in squamous cell carcinoma, which occurs in the neck, by interacting with MCM5 [40] . In addition, lncCPLC promotes colorectal resistance by regulating cancer progression via interaction with ZBTB [41] . In the present study, we demonstrated that SLC25A21-AS1 was widely presented in the nucleus and cytoplasm, with a more abundant accumulation in the cytoplasm, suggesting that it likely functions at the post-transcriptional level.…”

MLLT11-TRIL complex promotes the progression of endometrial cancer through PI3K/AKT/mTOR signaling pathway

“…Cell migration was assessed using a wound scratch healing assay using the previously described protocol. 23 , 24 First, monolayer cells were prepared. The cell density was 5 ~ 10 × 10 5 cells/ml were placed in 24 well plates (500 UL per well), and 10% fetal bovine serum was added to RMPI 1640 medium, cultured for 16 ~ 24 h to form monolayer cells.…”