Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression

Abstract

Background Hepatocellular carcinoma (HCC) is a common type of malignant human cancer with high morbidity and poor prognosis, causing numerous deaths per year worldwide. Growing evidence has been demonstrated that long non-coding RNAs (lncRNAs) are closely associated with hepatocarcinogenesis and metastasis. However, the roles, functions, and working mechanisms of most lncRNAs in HCC remain poorly defined. Methods Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of CCDC183-AS1 in HCC tissues and cell lines. Cell proliferation, migration and invasion ability were evaluated by CCK-8 and transwell assay, respectively. Animal experiments were used to explore the role of CCDC183-AS1 and miR-589-5p in vivo. Bioinformatic analysis, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were performed to confirm the regulatory relationship between CCDC183-AS1, miR-589-5p and SKP1. Results Significantly upregulated expression of CCDC183-AS1 was observed in both HCC tissues and cell lines. HCC patients with higher expression of CCDC183-AS1 had a poorer overall survival rate. Functionally, overexpression of CCDC183-AS1 markedly promoted HCC cell proliferation, migration and invasion in vitro and tumor growth and metastasis in vivo, whereas the downregulation of CCDC183-AS1 exerted opposite effects. MiR-589-5p inhibitor counteracted the proliferation, migration and invasion inhibitory effects induced by CCDC183-AS1 silencing. Mechanistically, CCDC183-AS1 acted as a ceRNA through sponging miR-589-5p to offset its inhibitory effect on the target gene SKP1, then promoted the tumorigenesis of HCC. Conclusions CCDC183-AS1 functions as an oncogene to promote HCC progression through the CCDC183-AS1/miR-589-5p/SKP1 axis. Our study provided a novel potential therapeutic target for HCC patients.

Downregulation of circ-UBAP2 ameliorates oxidative stress and dysfunctions of human retinal microvascular endothelial cells (hRMECs) via miR-589-5p/EGR1 axis

“…For instance, circRNA microarray analysis showed that hsa_circ_0016070 is correlated with pulmonary arterial hypertension in patients with chronic obstructive pulmonary disease [ 18 ]. circDNMT3B overexpression reduces the retinal acellular capillary number and attenuates visual impairment in diabetic rats [ 19 ]. In the present study, the expression of circ-UBAP2 was overexpressed in the vitreous humor of DR patients and HG-treated hRMECs, which was consistent with a previous study [ 20 ].…”

“…miR-589-5p was predicted and confirmed to be a potential target of circ-UBAP2. To date, miR-589-5p has been mainly investigated in oncogeneses, such as hepatocellular carcinoma [ 19 , 20 ], renal cell carcinoma [ 21 ], and laryngocarcinoma [ 22 ]. However, it has not been sufficiently explored in DR. Zou et al [ 23 ] confirmed that miR-589-5p targets the oxidative damage gene XPC-rs2229090 C allele in the age-related cataract (ARC), which might increase the risk of ARC.…”

Comprehensive Analysis of Pyroptosis-Related Long Noncoding RNA Immune Infiltration and Prediction of Prognosis in Patients with Colon Cancer

“…MYOSLID promotes the progression of osteosarcoma through the miR-1286/RAB13 axis [ 59 ], and MYOSLID plays a key role in stomach neoplasm through the miR-29c-3p-mcl-1 axis [ 60 ], but it has not been reported in CC. CCDC183-AS1 enhances hepatocellular carcinoma progression by regulating SKP1 expression via MIR-589-5P [ 61 ]. The miR-3127-5p/E2F7 axis increases CC cell proliferation and migration, and LINC00174 plays a role in this process [ 62 ].…”

Circular RNA circUBE2J2 acts as the sponge of microRNA-370-5P to suppress hepatocellular carcinoma progression

“…For miRNA, reverse transcriptions were performed using the Mir-X miRNA First-Strand Synthesis Kit (Takara, Japan). Real-time qPCR was performed as described previously [ 19 ]. U6 and GAPDH were used as endogenous controls for the detection of circRNA, miRNA, and mRNA expression, respectively.…”