Long Non-Coding RNA Cancer Susceptibility Candidate 9 Regulates the Malignant Biological Behavior of Nasopharyngeal Carcinoma Cells by Targeting miR-497-5p/Wnt3a/β-catenin Signaling Pathway

Abstract

Nasopharyngeal carcinoma (NPC) is a major kind of head and neck epithelial carcinoma. Increasing evidences reveal that long noncoding RNAs are considered as vital regulators in tumorigenesis and progression. Although previous studies have found that cancer susceptibility candidate 9 (CASC9) highly expresses in NPC, the underlying mechanisms need to be further studied. In this study, we found that CASC9 was overexpressed and associated with worse prognosis in NPC. CASC9 knockdown significantly inhibited the cell proliferation, migration and invasion in vitro and enhanced the sensitivity of tumor cells to cisplatin and paclitaxel. Mechanism research confirmed CASC9 regulated the malignant biological behavior of nasopharyngeal carcinoma cells by targeting miR-497-5p/Wnt3a/β-catenin signaling pathway. The present study might provide a novel mechanism for tumorigenesis and progression of NPC and contribute to the development of an effective molecular target therapy.

MicroRNAs and Gene Regulatory Networks Related to Cleft Lip and Palate

“…hsa-miR-665-3p decreases cell viability by apoptosis or suppresses cell proliferation through downregulation of target genes in various cells, namely BCL2 in human lung adenocarcinoma cells [ 48 ], NHEG1 in human neuroblastoma [ 49 ], TRIM24 in human castration-resistant prostate cancer [ 50 ], and FZD4 in human oral squamous cell carcinoma [ 51 ]. In addition, hsa-miR-497-5p inhibits cell proliferation through downregulation of target genes in several human cancer cells, e.g., MAPK1 in cervical cancer cells [ 52 ], PDL1 or SLC7A5 in human colorectal cancer cells [ 53 ], and WNT3A in human nasopharyngeal carcinoma cells [ 54 ]. Thus, miR-124-3p, miR-655-3p, and miR-497-5p may play a key role in cell proliferation as tumor suppressors in cancers and CL/P inducers in development.…”

SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells

“…It has been often dosgnosed at the advanced stage, and this made the high mortality among the patients with NPC [2,3]. Nowadays, the major treatments of NPC contain radiotherapy, chemotherapy, surgery and other comprehensive clinical treatments, while the outcomes remain not ideal [4]. In addition, tumor metastasis is known to cause the poor prognosis of patients with NPC [5,6].…”