Concurrent chemotherapy and radiotherapy is associated with a considerable survival benefit for patients with stage II NPC.

PET/CT detects more distant metastases than conventional staging in patients with NPC. The largest benefit in terms of cost and patient management was observed in the subgroup with N2-3 disease and EBV DNA ≥ 4,000 copies/mL.

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The scientific community has been seeking cost-competitive and green solvents with good dissolving capacity for the valorization of lignocellulosic biomass. At this point, deep eutectic solvents (DESs) are currently emerging as a new class of promising solvents that are generally liquid eutectic mixtures formed by self-association (or hydrogen-bonding interaction) of two or three components. DESs are attractive solvents for the fractionation (or pretreatment) of lignocellulose and the valorization of lignin, owing to the high solubility of lignin in DESs. DESs are also employed as effective media for the modification of cellulose to afford functionalized cellulosic materials, such as cellulose nanocrystals. More interestingly, biomassderived carbohydrates, such as fructose, can be used as one of the constituents of DESs and then dehydrated to 5-hydroxymethylfurfural in high yield. In this review, a comprehensive summary of recent contribution of DESs to the processing of lignocellulosic biomass and its derivatives is provided. Moreover, further discussion about the challenges of the application of DESs in biomass processing is presented.

In software testing, something which can verify the correctness of test case execution results is called an oracle. The oracle problem occurs when either an oracle does not exist, or exists but is too expensive to be used. Metamorphic testing is a testing approach which uses metamorphic relations, properties of the software under test represented in the form of relations among inputs and outputs of multiple executions, to help verify the correctness of a program. This paper presents new empirical evidence to support this approach, which has been used to alleviate the oracle problem in various applications and to enhance several software analysis and testing techniques. It has been observed that identification of a sufficient number of appropriate metamorphic relations for testing, even by inexperienced testers, was possible with a very small amount of training. Furthermore, the cost-effectiveness of the approach could be enhanced through the use of more diverse metamorphic relations. The empirical studies presented in this paper clearly show that a small number of diverse metamorphic relations, even those identified in an ad hoc manner, had a similar fault-detection capability to a test oracle, and could thus effectively help alleviate the oracle problem.

Background: Recent advances in experimental and computational technologies have fueled the development of many sophisticated bioinformatics programs. The correctness of such programs is crucial as incorrectly computed results may lead to wrong biological conclusion or misguide downstream experimentation. Common software testing procedures involve executing the target program with a set of test inputs and then verifying the correctness of the test outputs. However, due to the complexity of many bioinformatics programs, it is often difficult to verify the correctness of the test outputs. Therefore our ability to perform systematic software testing is greatly hindered.

BackgroundLong non-coding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. They are aberrantly expressed in many types of diseases. In this study, we aimed to investigate the lncRNA profiles in preeclampsia. Preeclampsia has been observed in patients with molar pregnancy where a fetus is absent, which demonstrate that the placenta is sufficient to cause this condition. Thus, we analyzed the lncRNA profiles in preeclampsia placentas. Methodology/Principal FindingsIn this study, we described the lncRNA profiles in six preeclampsia placentas (T) and five normal pregnancy placentas (N) using microarray. With abundant and varied probes accounting for 33,045 LncRNAs in our microarray, 28,443 lncRNAs that were expressed at a specific level were detected. From the data, we found 738 lncRNAs that were differentially expressed (≥1.5-fold-change) among preeclampsia placentas compared with controls. Coding-non-coding gene co-expression networks (CNC network) were constructed based on the correlation analysis between the differentially expressed lncRNAs and mRNAs. According to the CNC network and GO analysis of differentially expressed lncRNAs/mRNAs, we selected three lncRNAs to analyze the relationship between lncRNAs and preeclampsia. LOC391533, LOC284100, and CEACAMP8 were evaluated using qPCR in 40 preeclampsia placentas and 40 controls. These results revealed that three lncRNAs were aberrantly expressed in preeclampsia placentas compared with controls. Conclusions/SignificanceOur study is the first study to determine the genome-wide lncRNAs expression patterns in preeclampsia placenta using microarray. These results revealed that clusters of lncRNAs were aberrantly expressed in preeclampsia placenta compared with controls, which indicated that lncRNAs differentially expressed in preeclampsia placenta might play a partial or key role in preeclampsia development. Misregulation of LOC391533, LOC284100, and CEACAMP8 might contribute to the mechanism underlying preeclampsia. Taken together, this study may provide potential targets for the future treatment of preeclampsia and novel insights into preeclampsia biology.

A fundamental problem for software testing is the oracle problem, which means that in many practical situations, it is extremely expensive, if not impossible, to verify the test result given any possible program input. Metamorphic testing is an approach to alleviating the oracle problem. The key part of metamorphic testing is a set of necessary properties of the software under test, namely metamorphic relations. Metamorphic relations not only help generate test cases, but also provide a mechanism to partially verify the test results without the need of oracle. In most previous studies, metamorphic relations were identified manually by testers in an ad hoc way. There is no systematic methodology that helps us identify metamorphic relations. In this paper, we propose a simple method, namely, the composition of metamorphic relations, for systematically constructing new metamorphic relations based on the already identified metamorphic relations. We conduct a case study and show that new metamorphic relations can be easily constructed by compositing some existing metamorphic relations. It is also observed that the new metamorphic relations are very likely to deliver a higher cost-effectiveness of metamorphic testing than the original metamorphic relations.