Long non-coding RNA Bhmt-AS attenuates hepatic gluconeogenesis via modulation of Bhmt expression

Shen, Xuan; Zhang, Yajun; Zhang, Xu; Yao, Yiwei; Zheng, Yujie; Cui, Xianwei; Liu, Chang; Wang, Qian; Li, John

doi:10.1016/j.bbrc.2019.06.008

Cited by 14 publications

(11 citation statements)

References 26 publications

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“…TFAP2A is the antisense gene of TFAP2A‐AS1, which plays an important role in different types of cancers 16,17 . LncRNA Bhmt‐AS promoted hepatic gluconeogenesis via regulating Bhmt 18 . Chen et al found that lncRNA ZNF503‐AS1 induced differentiation of retinal pigment epithelium cells by regulating ZNF503 19 .…”

Section: Introductionmentioning

confidence: 99%

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Long noncoding RNA TFAP2A‐AS1 promotes oral squamous cell carcinoma cell growth and movement via competitively binding miR‐1297 and regulating TFAP2A expression

Yang

Niu

Cui

et al. 2022

Molecular Carcinogenesis

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Oral squamous cell carcinoma (OSCC) is an aggressive and common malignancy in the head and neck, characterized by poor prognosis and high incidence. This study aimed to investigate the role of long noncoding RNA TFAP2A-AS1 in OSCC. The competing endogenous RNA network of TFAP2A-AS1 was constructed by bioinformatics analysis. The expressions of miR-1297, TFAP2A-AS1, and TFAP2Awere measured by quantitative reverse transcription-polymerase chain reaction.The correlations of TFAP2A-AS1, miR-1297, and TFAP2A with clinicopathological characteristics of OSCC were assessed. RNA immunoprecipitation and dual-luciferase reporter assay were used to identify the target of miR-1297. Cell proliferation was measured by colony formation assay and [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. Transwell assay and wound healing assay were performed to assess cell movement. TFAP2A-AS1 and TFAP2A were upregulated in OSCC and their expression levels were positively correlated. The levels of TFAP2A-AS1, miR-1297, and TFAP2A were also associated with lymphatic metastasis and the tumor-node-metastasis (TNM) stage of OSCC patients. TFAP2A-AS1 acted as a miR-1297 sponge. OSCC cell growth and movement were inhibited by miR-1297.Changes in the miR-1297 expression abolished the effects of TFAP2A-AS1 on OSCC cells. Additionally, TFAP2A was a target of miR-1297. TFAP2A promoted OSCC cell growth and migration/invasion, indicating that TFAP2A mediated the effects of TFAP2A-AS1 and miR-1297. TFAP2A-AS1 exerts an oncogenic effect in OSCC via the TFAP2A-AS1/miR-1297/TFAP2A axis, which may provide new targets and strategies for OSCC treatments

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Section: Introductionmentioning

confidence: 99%

“…16,17 LncRNA Bhmt-AS promoted hepatic gluconeogenesis via regulating Bhmt. 18 Chen et al found that lncRNA ZNF503-AS1 induced differentiation of retinal pigment epithelium cells by regulating ZNF503. 19 Thus, we hypothesized that TFAP2A-AS1 could regulate TFAP2A by competitively binding miRNA.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA TFAP2A‐AS1 promotes oral squamous cell carcinoma cell growth and movement via competitively binding miR‐1297 and regulating TFAP2A expression

Yang

Niu

Cui

et al. 2022

Molecular Carcinogenesis

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“…Recently, lncRNAs have been confirmed to inhibit miRNA activity, increase miRNA target genes, and act as competitive endogenous RNAs (ceRNA) of miRNAs [ 24 ]. Further, the effect of lncRNAs in IR has been the focus of several studies [ 25 , 26 ]. Previous studies [ 27 , 28 ] by our group have demonstrated that RSV can improve hepatic IR by regulating lncRNA NONMMUT058999.2 and NONMMUT008655.2 in mouse models.…”

Section: Introductionmentioning

confidence: 99%

Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA

Liu

Zhang

Song

et al. 2022

International Journal of Endocrinology

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Long noncoding RNA (lncRNA) is a crucial factor in the progression of insulin resistance (IR). Resveratrol (RSV) exhibits promising therapeutic potential for IR. However, there are few studies on whether RSV improves IR through lncRNA. This study aimed to determine whether RSV could influence the expression of lncRNA and to elucidate the underlying mechanism. Mice were divided into three groups: control group, high-fat diet (HFD) group, and HFD + RSV group. We conducted a high-throughput sequencing analysis to detect lncRNA and mRNA expression signatures and the ceRNA-network in the skeletal muscles of mice that were fed an HFD to induce IR. Hierarchical clustering, gene enrichment, and gene ceRNA-network analyses were subsequently conducted. Differentially expressed lncRNAs were selected and validated via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The biological functions of the selected lncRNAs were investigated by silencing the target genes via lentivirus transfection of C2C12 mouse myotube cells. RSV treatment reversed the expression of 338 mRNAs and 629 lncRNAs in the skeletal muscles of mice with HFD-induced IR. The results of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes database analyses indicated that the differentially expressed mRNAs modulated type II diabetes mellitus. After validating randomly selected lncRNAs via RT-qPCR, we identified a novel lncRNA, NONMMUT044897.2, which was upregulated in the HFD group and reversed with RSV treatment. Additionally, NONMMUT044897.2 was proven to function as a ceRNA of microRNA- (miR-) 7051-5p. Suppressor of Cytokine Signaling 1 (SOCS1) was confirmed as a target of miR-7051-5p. We further performed lentivirus transfection to knock down NONMMUT044897.2 in vitro and found that NONMMUT044897.2 silenced SOCS1 and potentiated the insulin signaling pathway. Hence, RSV mimicked the silencing effect of lentivirus transfection on NONMMUT044897.2. Our study revealed that RSV reduced IR in mouse skeletal muscles via the regulation of NONMMUT044897.2.

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“…Growing research has shown that lncRNAs widely participate in plenty of developmental and physiological processes [12,13], and are strongly correlated with the genesis and development of diseases, including coronary artery diseases [14], cancers [15], and metabolic diseases [16,17]. Recently, the functions of lncRNAs in IR have gained a lot of attention [18,19].…”

Section: Introductionmentioning

confidence: 99%

Resveratrol improves skeletal muscle insulin resistance through downregulating lncRNA NONMMUT044897.2

Liu

Zhang

Song

et al. 2021

Preprint

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Background: Long non-coding RNA (lncRNA) has proved to be crucial factors in the progression of insulin resistance (IR). Resveratrol (RSV) exhibits promising therapeutic potential for the IR. Nonetheless, whether RSV could influence the expression of lncRNAs and the interaction mechanisms in IR remain unclear.Methods: We conducted high-throughput sequencing to detect the lncRNAs and mRNAs expression signatures and the co-expression network of lncRNAs and mRNAs in skeletal muscle after a high-fat diet (HFD)-induced IR mice model with or without RSV treatment, including hierarchical clustering, gene enrichment and gene co-expression networks analysis. Highly differentially expressed lncRNAs were selected and validated by RT-qPCR. Finally, the biological functions of the selected lncRNAs were investigated by silencing expressing the target genes through lentivirus transfection in C2C12 mouse myotubes cells.Results: We revealed that 338 mRNAs and 629 lncRNAs whose expression in skeletal muscle after a high-fat diet (HFD)-induced IR mice model was reversed by RSV treatment. Gene Ontology and Kyoto encyclopedia of genes and genomes databases indicated that the differential expression mRNAs modulate the insulin signaling pathway. After validating randomly selected lncRNAs via RT-qPCR, we found that lncRNA (NONMMUT044897.2) and Suppressor of Cytokine Signaling 1 (SOCS1) were up-regulated in the HFD group, and reversed by RSV treatment. Additionally, NONMMUT044897.2 was validated to function as a ceRNA of microRNA (miR)-7051-5p and SOCS1 was confirmed as a target for miR‑7051-5p. We further performed lentivirus transfection to knockdown NONMMUT044897.2 in vitro and found that NONMMUT044897.2 silence inactivated SOCS1 and promoted the insulin signaling pathway. Importantly, RSV could mimic the effects of silencing NONMMUT044897.2.Conclusion: Our study revealed that resveratrol improves skeletal muscle IR might be via regulation of NONMUT044897.2.

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Long non-coding RNA Bhmt-AS attenuates hepatic gluconeogenesis via modulation of Bhmt expression

Cited by 14 publications

References 26 publications

Long noncoding RNA TFAP2A‐AS1 promotes oral squamous cell carcinoma cell growth and movement via competitively binding miR‐1297 and regulating TFAP2A expression

Long noncoding RNA TFAP2A‐AS1 promotes oral squamous cell carcinoma cell growth and movement via competitively binding miR‐1297 and regulating TFAP2A expression

Resveratrol Alleviates Skeletal Muscle Insulin Resistance by Downregulating Long Noncoding RNA

Resveratrol improves skeletal muscle insulin resistance through downregulating lncRNA NONMMUT044897.2

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