Long-lived glycosyl-enzyme intermediate mimic produced by formate re-activation of a mutant endoglucanase lacking its catalytic nucleophile

Viladot, J.‐L.; Canals, Françesc; Batllori, Xavier; Planas, Antoni

doi:10.1042/0264-6021:3550079

Cited by 25 publications

(12 citation statements)

References 43 publications

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“…During experiments to investigate the mechanism of action of b-glycanase, a trisaccharide labeled with a 2,4-dinitrophenyl ring was used as the substrate to study the action of a mutant from Bacillus licheniformis. MALDI failed to produce a molecular ion because of fragmentation arising from photochemical reactions catalyzed by the chromogenic aglycone (Viladot et al, 2001). Studies on the mechanism of action of acarbose (29), a tetrasaccharide aamylase inhibitor with the structure of acarvosine (Acv) …”

Section: Glycosylation and Other Reaction Mechanismsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Harvey¹

2008

Mass Spectrometry Reviews

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This review is the second update of the original review on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates that was published in 1999. It covers fundamental aspects of the technique as applied to carbohydrates, fragmentation of carbohydrates, studies of specific carbohydrate types such as those from plant cell walls and those attached to proteins and lipids, studies of glycosyl-transferases and glycosidases, and studies where MALDI has been used to monitor products of chemical synthesis. Use of the technique shows a steady annual increase at the expense of older techniques such as FAB. There is an increasing emphasis on its use for examination of biological systems rather than on studies of fundamental aspects and method development and this is reflected by much of the work on applications appearing in tabular form.

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Section: Glycosylation and Other Reaction Mechanismsmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Harvey¹

2008

Mass Spectrometry Reviews

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“…Electrolyte samples (0.4 mL) were taken from the cathode compartment every 20 min during 2 h of electroreduction for a total of seven samples per run. The formate concentration of the samples was determined by using a Megazyme enzyme based spectroscopic kit (K‐Form) . The absorbance of each sample was determined at λ =340 nm by using a Shimadzu UV‐2450.…”

Section: Methodsmentioning

confidence: 99%

Tuning the Composition of Electrodeposited Bimetallic Tin–Lead Catalysts for Enhanced Activity and Durability in Carbon Dioxide Electroreduction to Formate

Moore

Gyenge

2017

ChemSusChem

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Bimetallic Sn-Pb catalysts with five different Sn/Pb atomic ratios were electrodeposited on Teflonated carbon paper and non-Teflonated carbon cloth using both fluoroborate- and oxide-containing deposition media to produce catalysts for the electrochemical reduction of CO (ERC) to formate (HCOO ). The interaction between catalyst composition, morphology, substrate, and deposition media was investigated by using cyclic voltammetry and constant potential electrolysis at -2.0 V versus Ag/AgCl for 2 h in 0.5 m KHCO . The catalysts were analyzed before and after electrolysis by using SEM and XRD to determine the mechanisms of Faradaic efficiency loss and degradation. Catalysts that are mainly Sn with 15-35 at % Pb generated Faradaic efficiencies up to 95 % with a stable performance. However, pure Sn catalysts showed high initial stage formate production rates but experienced an extensive (up to 30 %) decrease of the Faradaic efficiency. The XRD results demonstrated the presence of polycrystalline SnO after electrolysis using Sn-Pb catalysts with 35 at % Pb and its absence in the case of pure Sn. It is proposed that the presence of Pb (15-35 at %) in mainly Sn catalysts stabilized SnO , which is responsible for the enhanced Faradaic efficiency and catalytic durability in the ERC.

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“…) glucan forming spherulites; polymers for biomaterials [44,45] Tropaeolum majus xyloglucan endotransfer-…”

Section: Xllg-a-f [A]mentioning

confidence: 99%

Glycosynthases in Biocatalysis

et al. 2011

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Glycosynthases, engineered glycoside hydrolases that are able to synthesize glycans in quantitative yields without hydrolyzing them, are among the most interesting tools for the chemoenzymatic synthesis of carbohydrates that have been made available so far. From their invention in 1998, these enzymes have been developed enormously and demonstrated to be convenient alternatives to the more expensive glycosyltransferase. Glycosynthases have been proved to be efficient catalysts for the synthesis of oligosaccharides, polysaccharides, and glycoconjugates of various lengths containing different monosaccharides bound through a-and b-linkages, which have interesting applications. In this review we describe the impact that glycosynthases have had on the biocatalysis and biosynthesis of carbohydrates through the description of the novel activities produced and of the development of the glycosynthasebased process. In addition, we summarize the approaches that, according to the current literature, can be adopted to produce novel glycosynthase activities and to improve the existing process.

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Long-lived glycosyl-enzyme intermediate mimic produced by formate re-activation of a mutant endoglucanase lacking its catalytic nucleophile

Cited by 25 publications

References 43 publications

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update covering the period 2001–2002

Tuning the Composition of Electrodeposited Bimetallic Tin–Lead Catalysts for Enhanced Activity and Durability in Carbon Dioxide Electroreduction to Formate

Glycosynthases in Biocatalysis

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