Long Chain N-acyl Homoserine Lactone Production by Enterobacter sp. Isolated from Human Tongue Surfaces

Yin, Wai‐Fong; Purmal, Kathiravan; Chin, Shenyang; Chan, Xin-Yue; Chan, Kok-Gan

doi:10.3390/s121114307

Cited by 25 publications

(31 citation statements)

References 28 publications

(39 reference statements)

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“…We have also shown that Enterobacter sp. produces C8-HSL and C12-HSL [26]. Similarly, in this work, we showed that strain T2-2 also produced C8-HSL and C12-HSL (Figure 4).…”

Section: Resultssupporting

confidence: 84%

N-acyl Homoserine Lactone-Producing Pseudomonas putida Strain T2-2 from Human Tongue Surface

Chen

Chin

Tee

et al. 2013

Sensors

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Bacterial cell-to-cell communication (quorum sensing) refers to the regulation of bacterial gene expression in response to changes in microbial population density. Quorum sensing bacteria produce, release and respond to chemical signal molecules called autoinducers. Bacteria use two types of autoinducers, namely autoinducer-1 (AI-1) and autoinducer-2 (AI-2) where the former are N-acylhomoserine lactones and the latter is a product of the luxS gene. Most of the reported literatures show that the majority of oral bacteria use AI-2 for quorum sensing but rarely the AI-1 system. Here we report the isolation of Pseudomonas putida strain T2-2 from the oral cavity. Using high resolution mass spectrometry, it is shown that this isolate produced N-octanoylhomoserine lactone (C8-HSL) and N-dodecanoylhomoserine lactone (C12-HSL) molecules. This is the first report of the finding of quorum sensing of P. putida strain T2-2 isolated from the human tongue surface and their quorum sensing molecules were identified.

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“…We have also shown that Enterobacter sp. produces C8-HSL and C12-HSL [26]. Similarly, in this work, we showed that strain T2-2 also produced C8-HSL and C12-HSL (Figure 4).…”

Section: Resultssupporting

confidence: 84%

N-acyl Homoserine Lactone-Producing Pseudomonas putida Strain T2-2 from Human Tongue Surface

Chen

Chin

Tee

et al. 2013

Sensors

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“…isolated from chilled salmon and raw milk can produce a detectable amount of AHLs [85,86]. The quorum molecule was confirmed to be C12-HSL by high resolution mass spectrometry in a subsequent dorsal human tongue study [52]. In species that do not produce AHLs, such as E. cloacae GS1, a LuxR homolog, SdiA, has been detected.…”

Section: Quorum Sensing In Gram-negative Bacteriamentioning

confidence: 99%

Development of Quorum-Based Anti-Virulence Therapeutics Targeting Gram-Negative Bacterial Pathogens

Tay

Yew

2013

IJMS

103

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Quorum sensing is a cell density-dependent signaling phenomenon used by bacteria for coordination of population-wide phenotypes, such as expression of virulence genes, antibiotic resistance and biofilm formation. Lately, disruption of bacterial communication has emerged as an anti-virulence strategy with enormous therapeutic potential given the increasing incidences of drug resistance in pathogenic bacteria. The quorum quenching therapeutic approach promises a lower risk of resistance development, since interference with virulence generally does not affect the growth and fitness of the bacteria and, hence, does not exert an associated selection pressure for drug-resistant strains. With better understanding of bacterial communication networks and mechanisms, many quorum quenching methods have been developed against various clinically significant bacterial pathogens. In particular, Gram-negative bacteria are an important group of pathogens, because, collectively, they are responsible for the majority of hospital-acquired infections. Here, we discuss the current understanding of existing quorum sensing mechanisms and present important inhibitory strategies that have been developed against this group of pathogenic bacteria.

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“…As reported by Angelika and colleagues, E. sakazakii produces 3-oxo-C6-HSL and 3-oxo-C8-HSL, indicating its pathogenicity is achieved via cell-to-cell signaling molecules [33]. Other work done by Yin and colleagues further showed that a member of the Enterobacter genus exhibits long chain AHL production, namely of N -dodecanoyl- l -homoserine lactone (C12-HSL) [34]. The spent culture supernatant of M004 strain was analyzed using mass spectrometry analysis which confirmed the presence of 3-oxo-C6-HSL and 3-oxo-C8-HSL (Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Freshwater-Borne Bacteria Isolated from a Malaysian Rainforest Waterfall Exhibiting Quorum Sensing Properties

Tan

Yunos

Tan

et al. 2014

Sensors

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One obvious requirement for concerted action by a bacterial population is for an individual to be aware of and respond to the other individuals of the same species in order to form a response in unison. The term “quorum sensing” (QS) was coined to describe bacterial communication that is able to stimulate expression of a series of genes when the concentration of the signaling molecules has reached a threshold level. Here we report the isolation from aquatic environment of a bacterium that was later identified as Enterobacter sp.. Chromobacterium violaceum CV026 and Escherichia coli [pSB401] were used for preliminary screening of N-acyl homoserine lactone (AHL) production. The Enterobacter sp. isolated was shown to produce two types of AHLs as confirmed by analysis using high resolution tandem mass spectrometry. To the best of our knowledge, this is the first documentation of an Enterobacter sp. that produced both 3-oxo-C6-HSL and 3-oxo-C8-HSL as QS signaling molecules.

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Long Chain N-acyl Homoserine Lactone Production by Enterobacter sp. Isolated from Human Tongue Surfaces

Cited by 25 publications

References 28 publications

N-acyl Homoserine Lactone-Producing Pseudomonas putida Strain T2-2 from Human Tongue Surface

N-acyl Homoserine Lactone-Producing Pseudomonas putida Strain T2-2 from Human Tongue Surface

Development of Quorum-Based Anti-Virulence Therapeutics Targeting Gram-Negative Bacterial Pathogens

Freshwater-Borne Bacteria Isolated from a Malaysian Rainforest Waterfall Exhibiting Quorum Sensing Properties

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