Long-acting Oral Formulation of Doxycycline: In vitro-in vivo Correlation Studies

Abstract

The aim of this study was to establish and validate an in vitro-in vivo correlation that could predict the bioavailability of 4 oral long-acting formulations of doxycycline hyclate, acrylic acid and polymethacrylate matrices prepared using various proportions of doxycycline:acrylic acid:polymethacrylate for formulation 1 (1:0.25:0.0035), formulation 2 (1:0.5:0.0075), formulation 3 (1:1:0.015) and formulation 4 (1:2:0.0225) and a sample of doxycycline without excipients. In vitro dissolution profiles were obtained in phosphate buffer and hydrochloric acid media, which were fitted to several mathematical models. Plasma concentrations were obtained from 48 healthy dogs to achieve in vivo profiles. Therapeutic concentrations were observed for 60 h for formulation 1 and 4, 48 h for formulation 2 and 3 and 24 h for the sample of doxycycline. None of the pharmacokinetic parameter differed significantly between formulation 1 and 2 or between formulation 3 and 4; however, doxycycline differed significantly in comparison. The in vivo-in vitro correlation coefficient obtained from point-to-point analysis >0.999 for formulation 3 and 4 and >0.9581 for the others. To validate the model, the absorbed fractions for all formulations were estimated to predict plasma concentration profiles. In conclusion, adequate in vitro-in vivo correlation was established for longacting formulations of doxycycline indicating that in vitro dissolution tests could be used as a surrogate for bioavailability studies.