Logalization of metallothionein in the brain of macaca fascicularis.

Suzuki, Fumiko; Nakajima, Katsuyuki; Kawaharada, Umeko; Hara, Fumiko; Uehara, Kaku; Otaki, Noriko; Kimura, Masami; Tamura, Yukio

doi:10.1267/ahc.25.609

Cited by 19 publications

(15 citation statements)

References 21 publications

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“…The findings agreed with those reported in the brain of humans [3,27,28], monkeys [26], rats [14,32] and mice [12,14]. Intense MT-I & -II immunoreactivity was observed in astrocytes in the periventricular area and those around the blood vessels, indicating that MT-I & -II may play a role as a barrier against the toxic substances including metals.…”

Section: Discussionsupporting

confidence: 90%

“…Immunohistochemical localization of MT-I & -II in the brain has been reported in humans [3,27,28], monkeys [26], mice [12,14] and rats [14,32]; MT-I & -II were shown in choroid plexus epithelial cells, ependymal cells, pia mater, and astrocytes in the hypothalamus, hippocampus, cerebral cortex and white matter throughout the brain. With at 5 µm were stained with hematoxylin and eosin (HE) and periodic acid methenamine silver (PAM).…”

Section: Animalsmentioning

confidence: 93%

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Localization of Metallothioneins-I & -II and -III in the Brain of Aged Dog.

Kojima

Shimada

Morita

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. Localization of metallothionein (MT) -I & -II and MT-III and its significance in the brain aging in dogs were examined using immunohistological and molecular pathological techniques. MT-I & -II immunohistochemistry showed positive staining in the hypertrophic astrocytes throughout the aged dog brains; these MT-I & -II immunoreactive astrocytes were predominant in the cerebral cortex and around the blood vessels in the brain. These findings dominated in the brain regions with severe age-related morphological changes. In situ hybridization using MT-I mRNA riboprobes also demonstrated signals for MT-I mRNA in these hypertrophic astrocytes. Immunohistochemistry using a guinea pig antiserum against a synthetic polypeptide of canine MT-III demonstrated positive MT-III immunoreactivity predominantly in neurons in the Zn-rich regions such as hippocampus and parahippocampus. The findings were supported by in situ hybridization using MT-III mRNA riboprobes. Both MT-III immunoreactivity and signals for MT-III mRNA were demonstrated in neurons in the brain regardless of the intensity of the age-related changes. These results suggest, first, MT-I & -II may be induced in relation to the progress of the age-related morphological changes in the brain, playing an important role in the protection of the brain tissue from the toxic insults responsible for the brain aging, and second, MT-III may play a role in maintenance of Zn-related essential functions of the brain.-KEY WORDS: aging, brain, canine, in situ hybridization, metallothionein.

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Section: Discussionsupporting

confidence: 90%

Section: Animalsmentioning

confidence: 93%

Localization of Metallothioneins-I & -II and -III in the Brain of Aged Dog.

Kojima

Shimada

Morita

et al. 1999

The Journal of Veterinary Medical Science

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“…Immunohistochemically, the localization of MT has been demonstrated not only in the liver and kidney (2, 7), but also in various organs, such as the lung (5), intestine (4), testis (5), prostate (18), brain (11,13,17), breast (16) as well as in tumors (8,12,14,16).…”

mentioning

confidence: 99%

Metallothionein in the Human Breast.

Suzuki

Nakajima

Kawaharada

et al. 1993

Acta Histochem. Cytochem

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“…Immunohistochemically, the location of MT has been demonstrated not only in the liver and kidney (2,13,19) but also in the various organs such as respiratory epithelial cells of the lung (9), the surface columnar epithelial cells of the intestinal villi (7), Paneth cells of the small intestine, the Sertoli and interstitial cells of the testis (8), glandular epithelial cells of the prostate in both normal and Cd-exposed mammals (20), and astrocytes and ependymal cells of human and monkeys (15,16). In the present study we investigated the aging changes of the metallothionein in the human brain.…”

mentioning

confidence: 99%

Metallothionein in the human brain.

Suzuki

Nakajima

Kawaharada

et al. 1992

Acta Histochem. Cytochem

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The localization of metallothionein (MT), a heavy metal binding protein of low molecular weight, in the human brain was investigated by the immunohistochemical technique, and also the amounts of MT were assayed by radioimmunoassay.Immunohistochemically, MT was found in pia mater, ependymal cells, protoplasmic astrocytes and neuropil of the gray matter, and fibrous astrocytes of the white matter of the brain. Cytoplasm, processes and some nuclei of protoplasmic and fibrous astrocytes showed strong MT immunostaining.Vascular feet and adventitia of the blood vessels were also positive for MT immunostaining. With aging, MT deposited thickly in the pia mater and in the dilated perivascular spaces showing reticular appearance, especially in those, so-called etat crible of basal ganglia and centrum semiovale of hypertensive and aged cases. According to the radioimmunoassay, the amount of MT in the brain increased with age.

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Logalization of metallothionein in the brain of macaca fascicularis.

Cited by 19 publications

References 21 publications

Localization of Metallothioneins-I & -II and -III in the Brain of Aged Dog.

Localization of Metallothioneins-I & -II and -III in the Brain of Aged Dog.

Metallothionein in the Human Breast.

Metallothionein in the human brain.

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