Location of the mouse complement factor H gene (&lt;i&gt;cfh&lt;/i&gt;) by FISH analysis and replication R-banding

SummaryWe conducted comparative FISH analyses to investigate the chromosomal rearrangements that have occurred during the evolution of the rodent genus Apodemus, which inhabits broadleaf forests in the temperate zone of the Palaearctic region. Chromosome-specific painting probes of the laboratory mouse were hybridized to chromosomes of seven Apodemus species, A. agrarius, A. argenteus, A. gurkha, A. peninsulae, A. semotus, A. speciosus and A. sylvaticus, and homologous chromosomal regions were determined in the species for the study of karyotypic evolution. Differences in the hybridization patterns were found in nine pairs of autosomes among the seven species. The chromosomal location of the 5S rRNA genes on the telomeric region of chromosome 20 was highly conserved in all the species. In contrast, there was much wider variation in the location of the 18S-28S rRNA genes, although the 18S-28S rRNA genes were predominantly located on chromosomes 7, 8 and 12. Phylogenetic relationships of the seven Apodemus species were inferred from the chromosome rearrangements and the chromosomal distribution patterns of the 18S-28S rRNA genes. The karyotypic relationships correlated well with the molecular phylogeny, and A. semotus had the most highly conserved karyotype among the seven species.

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“…Preparation of R-banded chromosomes and FISH were performed as described by Matsuda et al (1992) and Matsuda & Chapman (1995).…”

Section: Animals Chromosome Preparation and Fishmentioning

confidence: 99%

Karyotypic Evolution of Apodemus (Muridae, Rodentia) Inferred from Comparative FISH Analyses

et al. 2004

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“…Preparation of R-banded chromosomes and FISH were performed as described by Matsuda et al (1992) and Matsuda & Chapman (1995). Mitogen-stimulated splenocytes were cultured in RPMI 1640 medium supplemented with 20% fetal bovine serum (FBS).…”

Section: Chromosome Preparation and Fishmentioning

confidence: 99%

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Matsubara

Nishida‐Umehara

Kuroiwa

et al. 2003

Chromosome Research

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Comparative chromosome painting was applied to the Indian spiny mouse (Mus platythrix) with mouse (M. musculus) chromosome-specific probes for understanding the process of chromosome rearrangements between the two species.The chromosome locations of the 5S and 18S-28S ribosomal RNA genes and the order of the 119 and Tcp-1 genes in the In(17)2 region of the t-complex were also compared.All the painting probes were successfully hybridized to the Indian spiny mouse chromosomes, and a total of 27 segments homologous to mouse chromosomes were identified. The comparative FISH analysis revealed that tandem fusion were major events in the chromosome evolution of the Indian spiny mouse. In addition, other types of chromosome rearrangements, i.e. reciprocal translocations and insertions, were also included.3

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“…Preparation of R-banded chromosomes and FISH were performed as previously described (Matsuda et al, 1992;Matsuda and Chapman, 1995). Mitogen-stimulated mouse and rat splenocyte culture was synchronized by thymidine block, and the incorporation of 5-bromodeoxyuridine during the late replication stage was made for di erential replication staining after the release of excessive thymidine.…”

Section: Chromosome Preparation and In Situ Hybridizationmentioning

confidence: 99%

Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains

Yan¹,

et al. 1999

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The UNC-51 serine/threonine kinase of C. elegans plays an essential role in axonal elongation, and unc-51 mutants exhibit uncoordinated movements. We have previously identi®ed mouse and human cDNAs encoding UNC-51-like kinase (ULK1). Here we report the identi®cation and characterization of the second murine member of this kinase family, ULK2. Mouse ULK2 cDNA encodes a putative polypeptide of 1033 aa which has an overall 52% and 33% amino acid identity to ULK1 and UNC-51, respectively. ULKs and UNC-51 share a typical domain structure of an amino-terminal kinase domain, a central proline/serine rich (PS) domain, and a carboxy-terminal (C) domain. Northern blot analysis showed that ULK2 mRNA is widely expressed in adult tissues. In situ hybridization analysis indicated that ULK2 mRNA is ubiquitously localized in premature as well as mature neurons in developing nervous system. ULK2 gene was mapped to mouse chromosome 11B1.3 and rat chromosome 10q23 by FISH. HA-tagged ULK2 expressed in COS7 cells had an apparent molecular size of *150 kDa and was autophosphorylated in vitro. Truncation mutants suggested that the autophosphorylation occurs in the PS domain. Although expression of ULK2 failed to rescue unc-51 mutant of C. elegans, a series of ULK2/UNC-51 chimeric kinases revealed that function of the kinase and PS domains are conserved among species, while the C domain acts in a speciesspeci®c manner. These results suggest that ULK2 is involved in a previously uncharacterized signaling pathway in mammalian cells.

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Location of the mouse complement factor H gene (<i>cfh</i>) by FISH analysis and replication R-banding

Cited by 203 publications

References 12 publications

Karyotypic Evolution of Apodemus (Muridae, Rodentia) Inferred from Comparative FISH Analyses

Karyotypic Evolution of Apodemus (Muridae, Rodentia) Inferred from Comparative FISH Analyses

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Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains

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