Location of stem cells of human hair follicles by clonal analysis

Rochat, Ariane; Kobayashi, Koichi; Barrandon, Yann

doi:10.1016/0092-8674(94)90383-2

Cited by 493 publications

(401 citation statements)

References 33 publications

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“…In order to clarify the function of a given gene specifically in the stem cell, it is necessary to distinguish stem cells from their progenitors or surrounding cells. Despite the difficulties in identifying stem cells in mammalian tissue, stem cells from several tissues in mice such as skin, blood and intestine have been identified by BrdU labeling [3][4][5]. Although their regulation has been studied using conditional knockout mice, the process is relatively slow and expensive.…”

Section: Introductionmentioning

confidence: 99%

The Drosophila ovary: an active stem cell community

2007

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Only a small number of cells in adult tissues (the stem cells) possess the ability to self-renew at every cell division, while producing differentiating daughter cells to maintain tissue homeostasis for an organism's lifetime. The Drosophila ovary harbors three different types of stem cell populations (germline stem cell (GSC), somatic stem cell (SSC) and escort stem cell (ESC)) located in a simple anatomical structure known as germarium, rendering it one of the best model systems for studying stem cell biology due to reliable stem cell identification and available sophisticated genetic tools for manipulating gene functions. Particularly, the niche for the GSC is among the first and best studied ones, and studies on the GSC and its niche have made many unique contributions to a better understanding of relationships between stem cells and their niche. So far, both the GSC and the SSC have been shown to be regulated by extrinsic factors originating from their niche and intrinsic factors functioning within. Multiple signaling pathways are required for controlling GSC and SSC self-renewal and differentiation, which provide unique opportunities to investigate how multiple signals from the niche are interpreted in the stem cell. Since the Drosophila ovary contains three types of stem cells, it also provides outstanding opportunities to study how multiple stem cells in a given tissue work collaboratively to contribute to tissue function and maintenance. This review highlights recent major advances in studying Drosophila ovarian stem cells and also discusses future directions and challenges.

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Section: Introductionmentioning

confidence: 99%

The Drosophila ovary: an active stem cell community

2007

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“…HF lineagetracing experiments using tetracycline-regulated green fluorescent protein (GFP)-tagged histone H2B (Tet-o-H2B-GFP) (Tumbar et al 2004) or the expression of reporter genes under the control of HF-specific promoters such as K15 (Morris et al 2004), Lgr5 (Jaks et al 2008), or K19 showed that the cells residing in the bulge and upper germ are responsible for HF regeneration during adult homeostasis. Bulge SCs isolated by microdissection (Rochat et al 1994;Oshima et al 2001) or by flow cytometry based on the expression of CD34 (Trempus et al 2003;Blanpain et al 2004) or K15-GFP (Morris et al 2004) and cultured in vitro produced large proliferative colonies that could be propagated for a long time.…”

Section: Bulge Stem Cellsmentioning

confidence: 99%

Development and Homeostasis of the Skin Epidermis

Sotiropoulou

Blanpain

2012

Cold Spring Harbor Perspectives in Biology

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SUMMARYThe skin epidermis is a stratified epithelium that forms a barrier that protects animals from dehydration, mechanical stress, and infections. The epidermis encompasses different appendages, such as the hair follicle (HF), the sebaceous gland (SG), the sweat gland, and the touch dome, that are essential for thermoregulation, sensing the environment, and influencing social behavior. The epidermis undergoes a constant turnover and distinct stem cells (SCs) are responsible for the homeostasis of the different epidermal compartments. Deregulation of the signaling pathways controlling the balance between renewal and differentiation often leads to cancer formation.

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“…Mammary epithelial cells also exhibit rotational motion (Kino-oka et al, 2004), which is required for sphere formation in three-dimensional culture (Tanner et al, 2012). In mammalian epidermis, keratinocyte stem cells are located in the basal layer of the interfollicular epidermis and epidermal appendages (Rochat et al, 1994; Jones in ITGA6, but not control shRNA-transduced two-cell colonies (Fig. 5 B).…”

Section: Discussionmentioning

confidence: 99%

“…Frozen keratinocytes were thawed and cultivated at clonal density on a feeder layer of irradiated or mitomycin C-treated 3T3-J2 cells, at 37°C and 10% CO 2 in a 3:1 mixture of the DMEM and Ham's F12 medium supplemented with 10% FCS, 5 µg/ml insulin, 0.4 µg/ml hydrocortisone, 10 10 M cholera toxin, and 2 × 10 9 M triiodothyronine, as described previously (Rheinwald and Green, 1975;Rochat et al, 1994). Human epidermal keratinocytes were also cultivated with CnT-PR medium (CELLn-TEC), EpiLife medium containing supplement S7 (Life Technologies), and MCDB153 medium containing bovine pituitary extract (Hashimoto et al, 1994).…”

Section: Cell Culturementioning

confidence: 99%