Location of ribosomal genes in the chromosomes of Anopheles darlingi and Anopheles nuneztovari (Diptera, Culicidae) from the Brazilian Amazon

Rafael, Míriam Silva; Tadei, Wanderli Pedro; Recco‐Pimentel, Shirlei Maria

doi:10.1590/s0074-02762003000500008

Cited by 18 publications

(19 citation statements)

References 31 publications

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“…mosomes was motivated by the need to map, in silico and in situ , the ESTs generated from the genome sequencing of this species (Rafael and others, unpublished data). This was triggered by the first mapping of ribosomal genes 12 and genes of single copy (Hsp70) in polytene chromosomes of A. darlingi populations of Manaus and Macapá regions, in the Brazilian Amazon. 13 In previous studies, photomaps were described for other species as Anopheles funestus , 29 Anopheles stephensi , [31][32][33] Anopheles gambiae , 34,35 Anopheles subpictus , 36 Anopheles albimanus , 37 Anopheles triannulatus , 38 Anopheles cruzii , and Anopheles belattor .…”

Section: Discussionmentioning

confidence: 99%

“…2,3 The relevance of A. darlingi as vector of malaria, its isoenzymes, hehavior, genetic structure, biology, metaphase karyotype, mapping, and chromosome polymorphism were demonstrated in several studies. [4][5][6][7][8][9][10][11][12][13][14] In South America, 17 Anopheles species are known as important vectors of malaria. Of those, more than two-thirds belong to the subgenus Nyssorhynchus , which contains 29 named species throughout the Neotropics.…”

Section: Anopheles Darlingimentioning

confidence: 99%

“…Salivary glands of fourth stage A. darlingi larvae were dissected and used to prepare chromosome slides. 12,13,27 Preparations of well-spread polytene chromosomes on 11 slides were photographed using a light microscope (Carl Zeiss Axioplan, Ulm, Germany), for further analysis and construction of a photomap.…”

Section: Sampling and Identification Of Mosquitoesmentioning

confidence: 99%

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Salivary Polytene Chromosome Map of Anopheles darlingi, the Main Vector of Neotropical Malaria

Rafael

Rohde²,

Bridi³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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Abstract. New photomap of Anopheles ( Nyssorhynchus ) darlingi Root, 1926, is described for a population from Guajará-Mirim, State of Rondonia, Brazil. The number of sections in the previous A. darlingi reference map was maintained and new subsections were added to the five chromosome arms. Breakage points of paracentric inversions had been previously incorporated into the photomap of this species. An additional inversion is reported, called 3Lc, totaling 14 inversions in the A. darlingi chromosome arms. The proposed photomap is potentially useful for further evolutionary studies in addition to physical and in silico chromosome mapping using A. darlingi genomic and transcriptome sequences. Furthermore, in our attempt to compare sections of the 2R chromosome arm of A. darlingi with Anopheles funestus , Anopheles stephensi , and Anopheles gambiae , we found great differences in the arrangement of the polytene chromosome bands, which are consistent with the known phylogenetic divergence of these species.

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Section: Discussionmentioning

confidence: 99%

Section: Anopheles Darlingimentioning

confidence: 99%

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Salivary Polytene Chromosome Map of Anopheles darlingi, the Main Vector of Neotropical Malaria

Rafael

Rohde²,

Bridi³

et al. 2010

The American Journal of Tropical Medicine and Hygiene

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“…Slides were processed with 2% acetic-orcein and Ag-NOR staining (Howell and Black, 1980), with minor modifications following Rafael et al (2003).…”

Section: Slide Preparation Nuclear Abnormalities and Chromosomal Abementioning

confidence: 99%

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

Wj²,

Jj³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Dillapiol, a phenylpropanoid isolate from essential oils of leaves of Piper aduncum (Piperaceae), has insecticidal, fungicidal and antimicrobial activities. The insecticidal activity of dil- Genotoxic effect of dillapiol on dengue mosquitoes lapiol was tested in vivo on the larvae and pupae of Aedes aegypti, the mosquito vector of dengue. Specifically, the effect of dillapiol on the formation of micronuclei and chromosome aberrations was analyzed. Dillapiol treatments comprised two concentrations of 200 and 400 μg/mL, dissolved in well water, and a pure well water control used to rear four generations of mosquitoes. Micronuclei occurred in mitotic diploid and tetraploid chromosomes of larvae; nuclear abnormalities also occurred in interphase, metaphase, telophase, and single nucleus cells of pupae. Mortality, oviposition, chromosome breakage, and anaphase bridges were significantly greater in the extract treatments than in controls. The genotoxic effects of dillapiol described here suggest that this natural product may be a useful alternative for the control of A. aegypti.

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“…The rRNA genes are located in the nucleolar organizer regions (NORs) and coincide with the location of constitutive heterochromatin in the sex chromosomes of 15 mosquitoes species from Italy, USA and Uganda (M archi and P ili 1994). Anopheles darlingi and Anopheles nuneztovari from the Brazilian Amazon have heteromorphic sex chromosomes with intraindividual variations in the size of the rRNA genes (a rDNA probe from Drosophila melanogaster ) located in the pericentromeric region; this location coincides with that of constitutive heterochromatin (R afael et al 2003).…”

mentioning

confidence: 99%

Cytogenetic study of Anopheles albitarsis (Diptera: Culicidae) by C-banding and in situ hybridization

et al. 2006

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The C-banding pattern and the size and location of the nucleolar organizer regions (NORs) are described for the first time in Brazilian populations of Anopheles (Nyssorhynchus ) albitarsis sensu lato. C-banding revealed variation in the size of the centromeric heterochromatic blocks in autosomal chromosomes and in the acrocentric (X) and puntiform (Y) sex chromosomes. Fluorescence in situ hybridization showed that the NORs were located in the pericentromeric region of the sex (XX/XY) chromosomes and that this coincided with the number and location of centromeric constitutive heterochromatin blocks previously revealed by C-banding. The NORs varied in size among the homologues of the three populations. These findings of the populations studied support the hypothesis that the stability of NORs in the A. albitarsis complex is characterized by the presence of clustered and conserved sites in a unique pair of chromosomes.

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Location of ribosomal genes in the chromosomes of Anopheles darlingi and Anopheles nuneztovari (Diptera, Culicidae) from the Brazilian Amazon

Cited by 18 publications

References 31 publications

Salivary Polytene Chromosome Map of Anopheles darlingi, the Main Vector of Neotropical Malaria

Salivary Polytene Chromosome Map of Anopheles darlingi, the Main Vector of Neotropical Malaria

Potential control of Aedes aegypti (Diptera: Culicidae) with Piper aduncum L. (Piperaceae) extracts demonstrated by chromosomal biomarkers and toxic effects on interphase nuclei

Cytogenetic study of Anopheles albitarsis (Diptera: Culicidae) by C-banding and in situ hybridization

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