Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo

Strobel, Klaus; Hoff, Joerg van den; Pietzsch, Jens

doi:10.1194/jlr.d700024-jlr200

Cited by 70 publications

(121 citation statements)

References 18 publications

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“…Furthermore, all lipid protons are involved in scalar coupling (J-coupling) interactions (21)(22)(23), which cause their response to PRESS to be modulated as a function of TE. Efforts have been made to correct for T 2 losses (11,12,18); however, to our knowledge signal correction for J-coupling evolution has not been performed when establishing lipid compositions using in vivo sequences. To demonstrate the influence of scalar coupling effects, we compare lipid compositions obtained from short-TE PRESS spectra, from short-TE PRESS spectra corrected for T 2 relaxation (18), and from spectra acquired with a PRESS sequence designed to minimize the effects of J-coupling (24,25).…”

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confidence: 99%

“…Efforts have been made to correct for T 2 losses (11,12,18); however, to our knowledge signal correction for J-coupling evolution has not been performed when establishing lipid compositions using in vivo sequences. To demonstrate the influence of scalar coupling effects, we compare lipid compositions obtained from short-TE PRESS spectra, from short-TE PRESS spectra corrected for T 2 relaxation (18), and from spectra acquired with a PRESS sequence designed to minimize the effects of J-coupling (24,25). The latter technique is applicable to spins involved only in weak-coupling interactions, and was recently applied to measure T 2 values of methyl lipid protons at 3 T (26).…”

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confidence: 99%

“…The oils studied in this work consisted primarily of saturated (SFA), monounsaturated (MUFA), and diunsaturated fatty acids (DUFA); specifically, the main MUFA and DUFA constituents are oleic and linoleic acid, respectively. It was assumed that the mouse adipose tissue was composed of SFA, MUFA, DUFA, in addition to a 2% fixed amount of triunsaturated fatty acids (TUFA) (18). The lipid compositions can be calculated from the NMR spectra by the following equations (14,18), Equation 1, where PUFA is the total amount of polyunsaturated fatty acids, and %TUFA ¼ 0 for the oils and 2% for the mouse.…”

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confidence: 99%

“…High-resolution NMR has been employed to identify the constituents of oils, animal fats, and extracts of rat adipose tissue (13)(14)(15)(16). Recently, in vivo MRS measurements of rodents (12,17,18) and humans (11) have been performed at high field strengths to calculate adipose tissue composition from the areas of the 2.1 ppm, 2.3 ppm, and 2.8 ppm (diallylic protons) proton lipid peaks. Previous studies (11,12,18) utilized the popular PRESS (point resolved spectroscopy) (19,20) pulse sequence for spatial localization.…”

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confidence: 99%

“…Recently, in vivo MRS measurements of rodents (12,17,18) and humans (11) have been performed at high field strengths to calculate adipose tissue composition from the areas of the 2.1 ppm, 2.3 ppm, and 2.8 ppm (diallylic protons) proton lipid peaks. Previous studies (11,12,18) utilized the popular PRESS (point resolved spectroscopy) (19,20) pulse sequence for spatial localization. During the PRESS echo time, TE, the signal of each lipid resonance decays due to transverse relaxation according to a T 2 constant characteristic of the protons contributing to that peak.…”

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confidence: 99%

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Effect of J‐coupling on lipid composition determination with localized proton magnetic resonance spectroscopy at 9.4 T

Yahya

Tessier²,

Fallone

2011

Magnetic Resonance Imaging

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Purpose: To demonstrate, at 9.4 T, that J-coupling interactions exhibited by lipid protons affects lipid composition determination with a point resolved spectroscopy (PRESS) sequence. Materials and Methods:Experiments were conducted on four oils (almond, corn, sesame, and sunflower), on visceral adipose tissue of a euthanized mouse, and on pure linoleic acid at 9.4 T. The 2.1, 2.3, and 2.8 ppm resonances were measured at multiple echo times (TEs) by a standard PRESS sequence and by a PRESS sequence consisting of narrow-bandwidth refocusing pulses designed to rewind the J-coupling evolution of the target peak protons in the voxel of interest. T 2 corrections were performed on both groups of data for the three peaks and lipid compositions for the oils and for the mouse tissue were determined. Lipid compositions were also calculated from a short-TE standard PRESS spectrum.Results: A chemical analysis of the samples was not performed; however, the oil compositions calculated from resonance peaks acquired with the PRESS sequence designed to minimize J-coupling effects, following T 2 relaxation correction, closely agreed with values in the literature, which was not the case for all of the compositions determined from the regular PRESS spectra. Conclusion:The presented work brings to attention the significance of J-coupling effects when calculating lipid compositions from localized proton spectra.

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confidence: 99%

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confidence: 99%

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Effect of J‐coupling on lipid composition determination with localized proton magnetic resonance spectroscopy at 9.4 T

Yahya

Tessier²,

Fallone

2011

Magnetic Resonance Imaging

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Body Fat MRS

Thomas

Bell

2015

eMagRes

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Dr E Louise Thomas obtained her PhD in vivo 13 C MRS from the University of London in 1996. She worked for many years at the Robert Steiner MRI Unit at the Hammersmith Hospital on the development and application of in vivo MR techniques to the study of adipose tissue composition, content and distribution. She has also worked extensively on the non-invasive study of ectopic fat, particularly liver fat and has published over 80 peerreviewed articles in this area. She has recently been appointed as a Senior Lecturer at the University of Westminster.Professor Jimmy D Bell completed his PhD in Biochemistry in 1987 (London). He has worked extensively on the development and application of in vivo MR techniques for the study of disease development, with especial focus on adipose tissue and liver metabolism. He joined the MRC Clinical Sciences Centre (Imperial College London) in the mid-1990s, where he was appointed Group Head. He recently moved to the University of Westminster to establish a new Research Centre for Optimal Health (ReCOH). He has published over 190 peer-reviewed papers and authored over a dozen chapters for scientific books.Keywords: body fat, adipose tissue, magnetic resonance imaging, magnetic resonance spectroscopy; bone marrow; visceral; subcutaneous; 2 AbstractThe increasing levels of obesity, and its associated co-morbidities, have prompted a reassessment of the techniques used for assessing body fat, including content, distribution and composition. Magnetic resonance spectroscopy (MRS) is amongst the many invaluable in vivo tools available today to evaluate the role of body fat in health and disease. However, although MRS has become a powerful technique for assessing ectopic fat in vivo, it has had limited use in other areas of research associated with body fat. MRS has found some success as a fast method to determine whole body adiposity in rodent models of disease, as well as a noninvasive method of obtaining an index of the overall composition of body fat in human subjects. Its more significant use has been in the understanding of bone marrow fat content, where important advances have been made, especially in longitudinal studies. In conclusion, in the area of body fat, MRS continues to be an adjunct technique to more precise and versatile MRI methods.

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Percutaneous thermal ablation of medium and large hepatocellular carcinoma

Yin

Xie

Lü

et al. 2009

Cancer

146

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Knowledge about the triglyceride (fat) 1H spectrum enables quantitative determination of several triglyceride characteristics. This work describes a model‐based chemical shift imaging method that separates water and fat signal and provides maps of three triglyceride quantities: fatty acid carbon chain length, unsaturation degree, and polyunsaturation degree. The method was validated by imaging a phantom containing 10 different oils using 1.5 and 3.0 T clinical scanners, with gas–liquid chromatography as reference. Repeated acquisitions demonstrated high reproducibility of the method. Statistical tests of correlation and linear regression were performed to examine the accuracy of the method. Significant correlation was found at both field strengths for all three quantities, and high correlation (r2 > 0.95) was found for measuring unsaturation degree and polyunsaturation degree. Feasibility of the method for in vivo imaging of the thigh was demonstrated at both field strengths. The estimates of unsaturation degree and polyunsaturation degree in subcutaneous adipose tissue were in agreement with literature values, while chain length appeared over estimated. The method has potential use in large‐scale cross‐sectional and longitudinal studies of triglyceride composition, and its relation to diet and various diseases. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.

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Localized proton magnetic resonance spectroscopy of lipids in adipose tissue at high spatial resolution in mice in vivo

Cited by 70 publications

References 18 publications

Effect of J‐coupling on lipid composition determination with localized proton magnetic resonance spectroscopy at 9.4 T

Effect of J‐coupling on lipid composition determination with localized proton magnetic resonance spectroscopy at 9.4 T

Body Fat MRS

Percutaneous thermal ablation of medium and large hepatocellular carcinoma

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