Localization to chromosomes of structural genes for the major protease inhibitors of barley grains

Hejgaard, Jørn; Se, Bjørn; Nielsen, Gunnar Gissel

doi:10.1007/bf00252327

Cited by 26 publications

(14 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The trypsin inhibitor sequenced by Odani et al (1983) and protein CMe (Salcedo et al 1984) had been found to be identical (Lázaro et al 1985), and the corresponding gene had been located in chromosome 3H (Hejgaard et al 1984;Salcedo et al 1984). Besides trypsin inhibitor CMe, a second homologous trypsin inhibitor, designated CMe, had been identified in barley endosperm (Barber et al 1986) and its gene assigned to chromosome 7H (Salcedo et al 1984).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

Rodrı́guez-Palenzuela¹,

Royo²,

Gómez³

et al. 1989

Mol Gen Genet

View full text Add to dashboard Cite

Summary.A cDNA encoding trypsin inhibitor CMe from barley endosperm has been cloned and characterized. The longest open reading frame of the cloned cDNA codes for a typical signal peptide of 24 residues followed by a sequence which is identical to the known amino acid sequence of the inhibitor, except for an lie/Leu substitution at position 59. Southern blot analysis of wheat-barley addition lines has shown that chromosome 3H of barley carries the gene for CMe. This protein is present at less than 2%-3% of the wild-type amount in the mature endosperm of the mutant Ris0 1508 with respect to Bomi barley, from which it has been derived, and the corresponding steady state levéis of the CMe mRNA are about 1%. One or two copies of the CMe gene (synonym Itcl) per haploid genome have been estimated both in the wild type and in the mutant, and DNA restriction patterns are identical in both stocks, so neither a change in copy number ñor a major rearrangement of the structural gene account for the markedly decreased expression. The mutation at the lys 3a locus in Ris0 1508 has been previously mapped in chromosome 7 (synonym 5H). A single dose of the wild-type alíele at this locus (Lys 3a) restores the expression of gene CMe (alíele CMe-1) in chromosome 3H to normal levéis.

show abstract

Section: Discussionmentioning

confidence: 99%

“…1. This chromosome had been previously proposed as the site of gene CMe, based on the electrophoretic analysis of the protein (Salcedo et al 1984;Hejgaard et al 1984). The insert of clone X CMe was subcloned and sequenced (Fig.…”

Section: Bgliimentioning

confidence: 99%

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

Rodrı́guez-Palenzuela¹,

Royo²,

Gómez³

et al. 1989

Mol Gen Genet

View full text Add to dashboard Cite

show abstract

“…It has previously been shown that the mutant has a deletion of most or all of the B hordein genes [40]. Since the CI-1 genes have been mapped to the long arm of chromosome 5 [28], it is unlikely that their expression is directly affected by the mutation. It is probable that the increased CI-1 compensates for the decreased B hordein because of an excess of soluble amino acids which accumulate in the seeds of mutant 56.…”

Section: Expression O F Ci-1 In Normal and Mutant Barleysmentioning

confidence: 99%

Molecular cloning of two isoinhibitor forms of chymotrypsin inhibitor 1 (CI-1) from barley endosperm and their expression in normal and mutant barleys

1988

View full text Add to dashboard Cite

Full-length cDNA clones for barley chymotrypsin inhibitor 1 (CI-1) have been isolated from an endosperm-specific cDNA library. Hybridization and nucleotide sequence analyses indicate that these cDNAs represent two distinct types of CI-1 mRNA which we have called CI-1A and CI-1B. Both mRNAs encode polypeptides of 83 residues (M r=8790 and 8960) which differ at eleven positions. The full-length cDNA sequences do not predict N-terminal signal peptide extensions indicating that CI-1 is synthesized in the mature form in contrast to the homologous proteinase inhibitors of tomato and potato. Northern hybridization experiments show that the CI-1 genes are under strict developmental and organ-specific control. CI-1 transcripts were first detected in the developing barley endosperm between 12 and 14 days after anthesis but no CI-1-related sequences were detected in the RNA preparations from shoots, leaves or roots. The expression of CI-1 was also studied in the high-lysine barley mutants Hiproly, Risø 56 and Risø 1508. Approximately 15-fold (Hiproly) and 4-fold (Risø 56 and 1508) higher levels of CI-1 mRNA were detected in the mutant endosperms compared to normal barley. These results correlate well with the increased deposition of CI-1 in the high-lysine lines and show that the differential expression is controlled mainly at the level of transcription or stability of the mRNA. Using Southern-blots of barley DNA we estimate that there are three copies of CI-1 per haploid genome in both normal and mutant barley lines.

show abstract

“…These traits are a potentially valuable resource of useful genes for the genetic improvement of wheat (Triticum aestivum, 2n = 42, AABBDD), a relative of barley. It has been reported that the basi gene on the long arm of barley chromosome 2H confers reduced precocious germination by coding an endogenous, bifunctional α-amylase/subtilisin inhibitor to modulate α-amylase activity (Hejgaard et al, 1984). It is especially effective at inhibiting wheat α-amylase activity (Henry et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat

Wang¹,

Zou²,

Lin³

et al. 2012

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining the wheat group 2 chromosomes of the EST database and the genome sequences of the new model plant Brachypodium distachyon. Seventy-seven of 120 primer pairs were polymorphic and 31 of 120 primer pairs were monomorphic between a set of wheat-barley chromosome 2H disomic addition/substitution lines and their parents by agarose gel electrophoresis and polyacrylamide gel electrophoresis. Thirty of 77 polymorphic primer pairs including primer pair P120 derived from the basi gene were chromosome 2H-specific. These markers are expected to be valuable in screening of wheat-barley

show abstract

Localization to chromosomes of structural genes for the major protease inhibitors of barley grains

Cited by 26 publications

References 20 publications

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

The gene for trypsin inhibitor CMe is regulated in trans by the lys 3a locus in the endosperm of barley (Hordeum vulgare L.)

Molecular cloning of two isoinhibitor forms of chymotrypsin inhibitor 1 (CI-1) from barley endosperm and their expression in normal and mutant barleys

Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat

Contact Info

Product

Resources

About