Localization of vasopressin-, vasoactive intestinal polypeptide-, peptide histidine isoleucine-and somatostatin-mRNA in rat suprachiasmatic nucleus

Card, J. Patrick; Fitzpatrick-McElligott, S; Gozes, Illana; Baldino, Frank

doi:10.1007/bf00214373

Cited by 111 publications

(29 citation statements)

References 43 publications

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“…Alternatively, differential protein processing may occur, resulting in cells expressing either VIP or PHI. The fact that all brain cells synthesizing VIP mRNA also contain PHM mRNA (Linder et al, 1987;Card et al, 1988) points out that the differential regulation could be at the protein processing level.…”

Section: B Vasoactive Intestinal Peptide Gene and Protein Structurementioning

confidence: 99%

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

Delgado

Pozo²,

Ganea³

2004

Pharmacol Rev

364

311

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Section: B Vasoactive Intestinal Peptide Gene and Protein Structurementioning

confidence: 99%

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

Delgado

Pozo²,

Ganea³

2004

Pharmacol Rev

364

311

View full text Add to dashboard Cite

“…In support of this view, 24-h rhythms of SCN vasopressin expression and locomotor activity were severely altered by the continuous administration of IFN-␣. The vasopressin of SCN origin is considered to transmit circadian output signals to other parts of the brain (Card et al, 1988;Daikoku et al, 1992). The impaired rhythm of vasopressin production in the SCN of IFN-␣-treated mice would reflect the attenuation of circadian output signals driven by SCN oscillatory functions; thus, such attenuated output signals from the central SCN pacemaker may contribute to the altered rhythm of locomotor activity during IFN-␣ treatment.…”

Section: Alteration Of Biological Rhythms Induced By Interferon-␣ 1397mentioning

confidence: 99%

Alteration of Intrinsic Biological Rhythms during Interferon Treatment and Its Possible Mechanism

Koyanagi

Ohdo

2002

Mol Pharmacol

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One of the most indispensable biological functions for all living organisms is the circadian clock, which acts like a multifunctional timer to regulate the homeostatic system, including sleep and wakefulness, hormonal secretions, and various other bodily functions with a 24-h cycle. We reported previously that interferon (IFN) has the ability to modulate the biological clock system at the genetic level. In the present study, this mechanism was investigated further by evaluating the effects of IFN-␣ on circadian output function. Treatment of cultured hepatic cells (HepG2) with IFN-␣ significantly decreased the protein levels of CLOCK and BMAL1, which are positive regulators of circadian output rhythm, then their mRNA levels. Aurintricarboxylic acid, a ligand inhibitor of IFN-␣, dose dependently inhibited the IFN-␣-induced phosphorylation of the signal transducer and activator of transcription 1 (STAT1) protein in HepG2 cells, accompanied by the restoration of Clock and Bmal1 mRNA levels. The continuous administration of IFN-␣ significantly decreased CLOCK and BMAL1 protein levels in the suprachiasmatic nucleus and liver of mice, thereby preventing oscillations in the expression of clock and clock-controlled output genes. These results reveal a possible pharmacological action by IFN-␣ on the core circadian oscillation mechanism and indicate that the disruptive effect of IFN-␣ on circadian output function is the underlying cause of its adverse effects on 24-h rhythms in physiology and behavior.

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“…This question could be approached directly if we could combine immunocytochemical methods that demonstrate the peptides with in situ hybridization histochernistry that labels specific mRNAs in individual neurons (Arentzen etal., 1985;Lewis etal., 1986;Goedert & Hunt, 1987;Rethelyi et al, 1987;Card et al, 1988;Fitzpatrick-McElligott et al, 1988;Baldino et al, 1988a).…”

Section: Introductionmentioning

confidence: 96%

In situ hybridization of mRNA for β-preprotachykinin and preprosomatostatin in adult rat dorsal root ganglia: comparison with immunocytochemical localization

et al. 1988

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In situ hybridization histochemistry was used to identify neurons in rat dorsal root ganglia that contained mRNAs encoding beta-preprotachykinin and preprosomatostatin. The distribution of these neurons was compared with the distribution of neurons containing tachykinins or somatostatin, identified using immunocytochemical techniques. Neurons labelled for beta-preprotachykinin mRNA constituted 20% of the total neuronal population and belonged to the small cell class. Neurons labelled for preprosomatostatin mRNA with either RNA or DNA hybridization probes constituted approximately 10% of the total cells and comprised a small cell group that differed in average size from the beta-preprotachykinin labelled population. The distribution of cells containing tachykinin- or somatostatin-like immunoreactive material was identical to the distribution of cells containing the respective mRNAs and, in addition, individual somata in adjacent sections contained both the mRNA precursor and the peptide. These results suggest that for these neuropeptides the sensitivity of the two methods is equivalent and the respective mRNAs and peptides are co-localized in the same neurons.

show abstract

Localization of vasopressin-, vasoactive intestinal polypeptide-, peptide histidine isoleucine-and somatostatin-mRNA in rat suprachiasmatic nucleus

Cited by 111 publications

References 43 publications

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

The Significance of Vasoactive Intestinal Peptide in Immunomodulation

Alteration of Intrinsic Biological Rhythms during Interferon Treatment and Its Possible Mechanism

In situ hybridization of mRNA for β-preprotachykinin and preprosomatostatin in adult rat dorsal root ganglia: comparison with immunocytochemical localization

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