In situ hybridization of mRNA for β-preprotachykinin and preprosomatostatin in adult rat dorsal root ganglia: comparison with immunocytochemical localization

Henken, Deborah B.; Tessler, Alan; Chesselet, Marie‐Françoise; Hudson, Alan P.; Baldino, Frank; Murray, Marion

doi:10.1007/bf01260994

Cited by 24 publications

(9 citation statements)

References 37 publications

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“…A similar qual- (Leonard et al, 1988;Oblinger et al, 1989;Wong and Oblinger, 1990) have been used effectively in semiquantitative studies, and it might be useful to employ these probes in size frequency histogram analyses such as that shown in Figure 4A for NF-L and peripherin. Such size frequency histogram analyses, using in situ hybridization histochemistry , have been done successfully for various neuropeptides and alphatubulin in the rat DRG (Henken et a]., 1988;Boehmer et al, 1989). However, our data (Table 1B) indicate that the neuropeptides, substance P and CGRP, cut across the cell size boundaries defined by the neurofilament and peripherin antigenicities.…”

Section: Discussioncontrasting

confidence: 56%

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NF‐L and peripherin immunoreactivities define distinct classes of rat sensory ganglion cells

Goldstein

House

Gainer

1991

J of Neuroscience Research

157

120

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Double immunofluorescence studies using antibodies against NF-L and peripherin revealed three distinct subpopulations of neurons in rat dorsal root ganglia (DRG). In the adult rat, 46% of the DRG neurons were small and peripherin-positive (NF-L-negative), and 48% were large and NF-L-positive (peripherin-negative). About 6% were both peripherin- and NF-L-positive. All of the DRG neurons reacted with antibodies to NF-M and nonphosphorylation-dependent or phosphorylation-independent antibodies to NF-H. The neuropeptides were predominantly found in the peripherin-positive small cell population. Eighty-seven percent of the peripherin-positive small cell population contained substance P immunoreactivity, while 43% of this cell population contained CGRP. In contrast, only 18-24% of the NF-L-positive large-cell population contained neuropeptides, and these were primarily in a smaller sized subpopulation. Similar patterns of antigen representation were observed in neonatal (PN2) DRG cell populations. Tissue cultures of sensory ganglion cells from PN2 DRG, in serum-free medium, stably maintained exclusively peripherin-positive neurons, with about 5% of these containing coexistent NF-L immunoreactivity. Very high levels of neuropeptide gene expression were exhibited by these postnatal neurons in culture.

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Section: Discussioncontrasting

confidence: 56%

“…2 in Szaro et al, 1990). Furthermore, in situ hybridization analysis of NF mRNA reveals its presence in the small cell perikarya, although at very low levels (Hoffman et al, 1987;Wong and Oblinger, 1987;Verge et al, 1990). The reverse situation is the case for peripherin.…”

Section: Discussionmentioning

confidence: 94%

NF‐L and peripherin immunoreactivities define distinct classes of rat sensory ganglion cells

Goldstein

House

Gainer

1991

J of Neuroscience Research

157

120

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“…The IncStar antiserum crossreacts with several tachykinins (Henken, Tessler, Chesselet, Hudson, Baldino & Murray, 1988). The Peninsula antiserum is claimed by the vendor to have less than 1 % cross-reactivity with either neurokinin A or B.…”

Section: Discussionmentioning

confidence: 99%

Substance P mediates synaptic transmission between rat myenteric neurones in cell culture.

Willard

1990

The Journal of Physiology

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SUMMARY1. Whole-cell patch-clamp recordings were made from pairs of neurones in cell cultures of rat myenteric neurones. In some pairs, action potentials evoked in the first neurone evoked a slow excitatory postsynaptic potential (EPSP) in the second neurone.2. Action potentials at a frequency of at least 5 Hz were required to evoke slow EPSPs. In one group of cells, the slow EPSP followed a series of nicotinic fast EPSPs; in another group, fast EPSPs did not precede the slow EPSP.3. The slow EPSPs were 2-16 mV in amplitude and were accompanied by decreased resting potassium conductance.4. Most (17/28) neurones in which action potentials evoked only slow EPSPs in a follower cell contained substance P (SP)-like immunoreactivity; they were not immunoreactive for 5-hydroxytryptamine (0/15) or vasoactive intestinal peptide (0/22

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“…Previous reports have shown that SOM-LI and SOM-mRNA are present in only a small number of primary afferents. In rats, approximately 10% of all dorsal root ganglion cells were immunopositive Molander et al, 1987;Henken et al, 1988) or showed a positive reaction after in situ hybridization (Henken et al, 1988). All these studies were performed without colchicine treatment of the ganglia.…”

Section: Distribution Of Som-li In Primary Afferentsmentioning

confidence: 99%

Somatostatin‐like immunoreactivity in primary afferents of the medial articular nerve and colocalization with substance P in the cat

Hanesch

Heppelmann

Schmidt

1995

J of Comparative Neurology

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The proportion of somatostatin-containing dorsal root ganglion cells innervating the knee joint of the cat via the medial articular nerve was determined by using retrograde labeling with fast blue and immunohistochemistry. Immunoreactivity was found in 8.6% of labeled cell bodies. In colchicine-treated ganglia, the proportion increased to 16.8%. Only small and intermediate-sized perikarya showed somatostatin-like immunoreactivity, indicating that this neuropeptide is synthesized predominantly in primary afferent units with unmyelinated sensory axons but may also be present in primary afferents with thinly myelinated sensory fibers. Colchicine treatment had no influence on the cell size distribution. Colocalization of somatostatin with substance P was determined by comparing the proportions of immunopositive dorsal root ganglion cells after incubation with antibodies against substance P or somatostatin or with a mixture of both. Substance P-like immunoreactivity was found in 18.1% (untreated ganglia) and 19.6% (colchicine treated ganglia) of the labeled neurons. After incubation with a mixed antibody solution, 18.2% of joint afferents in untreated and 19.9% of the cells in colchicine-treated ganglia were immunopositive. Comparing this result with the results obtained using somatostatin and substance P antibodies alone, one can calculate that both neuropeptides are colocalized in about 17% of the cat's knee joint afferents. About 3% of the neurons contain only substance P, whereas almost none of the neurons contain only somatostatin. Based on this fact, one can assume that both neuropeptides are coreleased in peripheral tissue as well as in the central nervous system.

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In situ hybridization of mRNA for β-preprotachykinin and preprosomatostatin in adult rat dorsal root ganglia: comparison with immunocytochemical localization

Cited by 24 publications

References 37 publications

NF‐L and peripherin immunoreactivities define distinct classes of rat sensory ganglion cells

NF‐L and peripherin immunoreactivities define distinct classes of rat sensory ganglion cells

Substance P mediates synaptic transmission between rat myenteric neurones in cell culture.

Somatostatin‐like immunoreactivity in primary afferents of the medial articular nerve and colocalization with substance P in the cat

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