Localization of uridine monophosphate synthase (UMPS) gene to river buffalo chromosomes by FISH

Abstract

Uridine monophosphate synthase plays an important role in pyrimidine synthesis, converting orotic acid to uridine 5' monophosphate. In cattle, UMPS deficiency is inherited as a monogenic autosomal recessive trait. While heterozygous carriers are phenotypically normal, homozygotes are lethal in utero (Shanks et al. 1992). UMPS has been mapped to human chromosome 3q13 (Qumsiyeh et al. 1989), sheep chromosome 1q (Burkin et al. 1993) and cattle chromosome 1q31 (Ryan et al. 1994). In the present study we used a cattle genomic probe to localize UMPS to river buffalo chromosomes by fluorescence in situ hybridization.

Use of molecular markers for the identification of River Buffalo chromosomes: chromosome one

“…Cytological studies of buffalo chromosome one (BBU1) indicated that BBU1 q is homologous to BTA 1 (I annuzzi et al 1990). This was confirmed by the assignment of UMPS to buffalo BBU1q (I annuzzi et al 1994) which has been previously assigned to cattle U10 BTA 1 (R yan et al 1994). However, regarding the small acrocentric chromosome presenting the short arm there is still some controversy.…”

Gene mapping in the river buffalo (Bubalus bubalis L.): five syntenic groups

“…The major histocompatibility complex (MHC) was assigned to BTA 23 (F ries et al 1991; H ediger et al 1991; I annuzzi et al 1993a) and BBU 2p (I annuzzi et al 1993a). UMPS gene was first localized on BTA 1q31 (R yan et al 1994) and then on BBU 1q13 (I annuzzi et al 1994).…”

“…It is then speculated that the investigated syntenic groups can be assigned to buffalo chromosomes BBU 10, BBU 1q, BBU 14, BBU 9, and BBU 15, respectively. The gene for UMPS (U10) has already been assigned to BBU 1q, using in situ hybridization (I annuzzi et al 1994).…”

“…The first syntenic groups in the buffalo were recorded by de H ondt et al (1991). Isozyme markers have been assigned to chromosomes using somatic cell hybrids (E l N ahas et al 1993), by in situ hybridization using non‐radioactive labelling (I annuzzi et al 1993a, b, 1994) and radioactive labelling (H assanan et al 1993, 1994). This work is a contribution to the development of the river buffalo gene map, analysing the segregation of 10 bovine loci by testing the PCR products of the corresponding primers in a panel of 45 buffalo‐hamster somatic cell hybrids.…”